[目的]了解山东省菏泽地区奶牛肠道寄生虫感染现状,为奶牛寄生虫防控提供参考。[方法]在菏泽地区选择8 家养殖场,按犊牛（<6 月龄）、育成牛（7~16 月龄）、成年牛（>16 月龄）分类,采用直肠法收集奶牛新鲜粪便1 438 份,利用离心沉淀法、饱和蔗糖漂浮法及卢戈氏碘液染色法,进行显微镜检测。[结果]寄生虫总感染率33.45%;球虫、阿米巴原虫、隐孢子虫、圆线虫、贾第虫、绦虫、鞭虫感染率分别为27.33%、 7.65%、 3.27%、3.48%、0.56%、0.49%、1.32%;优势感染虫种为球虫;混合感染率为10.85%,混合虫体种类2~4 个。各场点和年龄阶段奶牛均感染寄生虫。[结论]菏泽地区奶牛肠道寄生虫感染较普遍,养殖场需采取防控措施。

[Objective]This study was conducted to investigate the status of intestinal parasite infection in dairy cows in Heze area of Shandong Province,and to provide reference for the prevention and control of dairy cow parasites.[Method]1 438 samples of fresh feces were collected by rectal method from 8 breeding farms in Heze area according to different age stages of calves （<6 months old）,mature cows （7~16 months old）and adult cows （>16 months old）,and were detected by centrifugal precipitation,saturated sucrose flotation and Lugo's iodine staining.[Result]The total parasite infection rate was 33.45%.They were coccidia,amoeba,cryptosporidium,strongylus,giardia,tapeworm and whipworm,and the infection rates were 27.33%,7.65%,3.27%,3.48%,0.56%,0.49%,1.32%,respectively. Coccidia was the dominant infection species. The mixed infection rate was 10.85%,and the mixed species ranged from 2 to 4. Cows at all sites and age stages were infected with the parasite.[Conclusion]Intestinal parasite infection was common in cows in Heze area,and necessary prevention and control measures should be taken.