[目的]对小白鼠进行最小免疫剂量及抗体消长规律初步研究。[方法]采用课题组制备的牛支原体灭活氢氧化铝佐剂疫苗,分别以0.1 mL/只·天、0.2 mL/只·天、0.3 mL/只·天、0.4 mL/只·天的不同剂量,注射小白鼠腿部肌肉。[结果]最小免疫剂量为0.2 mL/只;免疫3 周后抗体滴度达到最高峰,随后抗体滴度开始逐步下降,但仍保持相对稳定,抗体滴度维持在0.319的较高水平,5 周后抗体滴度表现明显下降趋势。[结论]本试验为后期牛支原体灭活疫苗在牛群中进行试验研究奠定了基础。

[Objective] The minimum immune dose and the fluctuation of antibodies of Mycoplasma bovis inactivated vaccine prepared by our research group were preliminarily studied.[Method] The prepared vaccine was injected into the leg muscles of mice at different doses of 0.1 mL/piece,0.2 mL/piece,0.3 mL/piece and 0.4 mL/piece.[Result] The minimum immune dose was 0.2 mL/piece. The antibody titer reached its peak 3 weeks after immunizing mice with the minimum dose,and then began to decline,but remained relatively stable.5 weeks later,the antibody titer showed a downward trend.[Conclusion] This study laid a foundation for the later application of mice immunized with Mycoplasma bovis inactivated vaccine in cattle.