[目的]目前国内尚无酵母β-葡聚糖在乳制品中定量检测方法的国家标准和行业标准。本文利用离子色谱法建立快速、准确的乳制品中酵母β-葡聚糖检测方法。[方法]样品加入沉淀剂离心后,倒去上清液,反复数次至无干扰,加入1 mol/L盐酸溶液,经121 ℃ 60 min酸水解,调节pH值稀释后,过钠柱,用离子色谱仪测定。[结果]通过对水洗次数、色谱柱选择等因素选择优化,葡萄糖在1~20 μg/mL浓度范围内线性关系良好,相关系数R²>0.999,方法检出限为5 mg/100 g,定量限为15 mg/100 g;葡萄糖的回收率为84.8%~94.9%,相对标准偏差为2.34%（n=6）。[结论]该方法稳定,定量准确,适用于乳制品中酵母β-葡聚糖含量的检测。

[Objective] At present,there is no national standard and industry standard for the quantitative detection of yeast β-glucan in dairy products. A rapid and accurate method for the determination of yeast β-glucan in dairy products was developed by ion chromatography.[Method] After the sample was centrifuged with precipitant,the supernatant was poured off,repeated several times until there was no interference,1 mol/L hydrochloric acid solution was added,acidized at 121 ℃ for 60 min,the pH value was adjusted and diluted,and then sodium column was used for determination by ion chromatograph.[Result] The linear relationship of glucose was good in the concentration range of 1~20 μg/mL with correlation coefficient R²>0.999. The detection limit and quantitation limit of the method were 5 mg/100 g and 15 mg/100 g respectively. The recovery of glucose was 81.5%~94.9%,the relative standard deviation was 2.34%（n=6）.[Conclusion] The method is stable,accurate and suitable for the determination of yeast β-glucan in dairy products.