新孢子虫是造成怀孕母牛流产等繁殖障碍和新生犊牛运动障碍的重要寄生虫之一,目前尚未有有效的药物和疫苗来治疗和预防牛新孢子虫病。本研究使用新孢子虫抗体检测试剂盒对采自福建省漳州、南平、三明和龙岩4 个地区规模化奶牛场的1 472 份奶牛血清样品进行抗体检测,并通过SPSS 22.0软件进行生物统计学分析。结果表明,血清样品新孢子虫抗体总阳性率为7.47%,4 个地区样品阳性率分别为5.57%、5.12%、7.41%和12.97%;不同年龄阶段奶牛阳性率范围为2.93%~9.56%,不同胎次阳性率范围为4.83%~9.50%。经统计学分析,不同年龄阶段奶牛阳性率差异显著（P<0.05）。这表明福建省奶牛普遍存在新孢子虫感染,奶牛场应采取综合防控策略和制定有效的管理措施控制奶牛新孢子虫病。
Neospora caninum is one of the important parasites that cause reproductive disorders such as miscarriage in pregnant cows and movement disorders in newborn calves. Currently, there are no effective drugs or vaccines to treat and prevent bovine neosporidiosis. This study used a commercialized ELISA kit to detect Neospora caninum antibodies in 1472 serum samples from large-scale dairy farms in Zhangzhou, Nanping, Sanming, and Longyan areas of Fujian Province. The results were analyzed using SPSS 22.0 software for biostatistics. The research results showed that the total antibodies positive rate of dairy cattle Neospora caninum was 7.47%. The Neospora caninum antibodies positive rates of samples from four regions were 5.57%, 5.12%, 7.41%, and 12.97%, respectively; The positive rates of dairy cattle Neospora caninum antibody in cows of different age groups range from 2.93% to 9.56%, and the positive rates of dairy cattle Neospora caninum antibody in cows of different parity ranges from 4.83% to 9.50%. By statistical analysis, there was a significant difference in the positivity rate of Neospora caninum antibody in cows at different age stages (P<0.05). The results of the present study showed that there is a widespread infection of Neospora caninum in dairy cattle in Fujian Province. Cattle farms should adopt comprehensive prevention and control strategies and develop effective management measures to control Neosporosis in cattle.
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