[目的]诊治某奶牛养殖场奶牛环形泰勒焦虫病。[方法]对香河县某奶牛养殖场患病奶牛采取流行病学调查、临床观察、血液涂片和PCR检测方法进行环形泰勒焦虫病诊断。[结果]该次疫情发病率31.25%,死亡率20.00%。患病奶牛皮肤多处分布蜱虫,临床主要表现包括发热、贫血、消瘦和体表淋巴结肿大等。镜检可见红细胞边缘呈棱角状,胞内充满寄生虫体,虫体多位于红细胞边缘,呈椭圆形、环形、梨形。利用特异性引物F/R对提取DNA进行PCR扩增,得到长度为750 bp扩增条带。该次疫情是由环形泰勒焦虫感染所致环形泰勒焦虫病。经采取中西药结合疗法,有效控制疫情蔓延。[结论]奶牛环形泰勒焦虫病要早发现,早治疗,消灭蜱虫,保持环境卫生,有效切断其传播途径,遏制疫情发展。

[Objective] The aim was to diagnose and treat dairy cow with Theileria annulata disease in a dairy farm. [Method] The epidemiological investigation,clinical observation, blood smear and PCR detection methods were used to diagnose the Theileria annulata disease in the diseased milk of a dairy farm in Xianghe County. [Results] The incidence rate of the epidemic was 31.25 %,and the mortality rate was 20.00 %. Ticks are distributed in many parts of the skin of diseased cows. The main clinical manifestations include fever,anemia,emaciation and surface lymphadenopathy. Microscopic examination showed that the edge of red blood cells was angular, and the cells were filled with parasites. The parasites were mostly located at the edge of red blood cells,which were oval,annular and pear-shaped. The specific primer F / R was used to amplify the extracted DNA by PCR, and a 750 bp amplified band was obtained. The epidemic was caused by the infection of Theileria annulata. The combination of Chinese and Western medicine was adopted to effectively control the spread of the epidemic. [Conclusion] Early detection,early treatment,elimination of ticks,maintaining environmental hygiene,effectively cutting off its transmission route and curbing the development of the epidemic should be carried out.