以我国关中地区中国荷斯坦牛与奶山羊为研究对象,基于非靶向脂质组学技术,分析乳脂肪球膜脂质,筛选膜上差异脂质。结果表明,在牛羊乳MFGM中分别检测到3 004 种和2 857 种脂质。牛乳中磷脂、糖脂、固醇和鞘脂分别占除甘油酯外其他脂质总含量75.94%、8.29%、3.82%和3.76%,羊乳中分别为66.98%、12.42%、4.27%、3.57%。此外,牛羊MFGM上存在1 496 种显著性差异脂质,其中TG（17︰0/10︰0/22︰6）、TG（16︰0e/8︰0/18︰1）、TG（8︰0/10︰4/14︰2）、TG（14︰0/14︰0/22︰6）和DG（15︰0/16︰0）等甘油酯分子与CL（87︰12）、PE（41︰2e）、SM（t39︰7）、CL（66︰1）、MePC（29︰2e）、PE（16︰0/16︰1）和PI（18︰1/20︰3）等MFGM脂质分子可用于牛羊乳分类鉴定以及区别检测靶点,为牛羊乳脂质检测提供理论基础。
Chinese Holstein cows and dairy goats in Guanzhong region of China were used as research subjects to analyze milk fat globule membrane lipids based on non-targeted lipidomics technology to screen for membrane differential lipids.The results showed that 3 004 and 2 857 lipids were detected in cow and goat milk MFGM,respectively.Phospholipids,glycolipids,sterols and sphingolipids accounted for 75.94%,8.29%,3.82% and 3.76% of the total content of lipids other than glycerides in cow's milk as compared to 66.98%,12.42%,4.27%,and 3.57% in goat's milk,respectively.In addition,1 496 significantly differentiated lipids were present on bovine and sheep MFGM,in which glyceride molecules such as TG（17︰0/10︰0/22︰6）,TG（16︰0e/8︰0/18︰1）,TG（8︰0/10︰4/14︰2）,TG（14︰0/14︰0/22︰6）,and DG（15︰0/16︰0） were associated with the same lipid profile as CL（87︰12）,PE（41︰2e）,SM（t39︰7）,CL（66︰1）,MePC（29︰2e）,MFGM lipid molecules such as PE（16︰0/16︰1） and PI（18︰1/20︰3） can be used for categorization and identification of cow's and sheep's milk as well as differentiation of the targets of the assay,which can provide the theoretical basis of the detection of cow's and sheep's milk lipids.
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