[目的] 以关中奶山羊为研究对象,通过模拟奶山羊繁殖季节光照条件,研究光照控制对奶山羊血液中褪黑素(MLT)及性激素分泌的影响。[方法] 试验选取26 只关中奶山羊做为试验组,控制舍内光照时间从16 h光照、8 h黑暗逐渐过渡到8 h光照、16 h黑暗。采用ELISA试剂盒测定母羊血液中MLT和促黄体生成素(LH)、垂体促卵泡素(FSH)与雌二醇(E2)水平。[结果] 通过一段时间短光照可以显著提高试验组奶山羊血液中MLT、FSH、LH、E2的浓度(P<0.05),本研究结果显示通过人为光照控制可以调节奶山羊性腺轴活动,提升血液中性激素浓度。[结论] 非繁殖季节光照诱导发情简便易行,成本低,有利于在规模化奶山羊场推广运用。
[Objective] In this study,Guanzhong dairy goats were used as the research object. The effects of light control on the secretion of melatonin and sex hormones in the blood of dairy goats were studied by simulating the light conditions in the breeding season of dairy goats. [Method] This experiment selected 26 Guanzhong dairy goats as the experimental group,and controlled the lighting time in the shed to gradually transition from 16 hours of light and 8 hours of darkness to 8 hours of light and 16 hours of darkness. The levels of MLT,uteinizing hormone (LH),follicle stimulating hormone (FSH),and estradiol(E2) in the blood of ewes were measured using an ELISA kit. [Result] The concentration of MLT,FSH,LH and E2 in the blood of dairy goats in the experimental group could be significantly increased by a period of short light (P< 0.05). The results of this study suggested that light control could regulate the activity of gonadal axis of dairy goats and increase the concentration of sex hormones in blood. [Conclusion] It can be seen that light induced estrus during non breeding seasons is simple,feasible,and cost-effective,which is conducive to its promotion and application in large-scale dairy goat farms.
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