[目的] 研究茶多酚和植物甾醇复合物的生物学功能及其对泌乳中后期奶牛采食量、产奶性能和血清生化指标的影响。[方法] 试验选取60 头年龄、胎次、泌乳时间和产奶量相近的健康奶牛,随机分为2 组,每组30 头。对照组饲喂基础日粮,试验组在基础日粮中添加20 g/d植物提取物,预试期7 d,正试期70 d。试验采集奶牛乳样和血样,测定乳成分和血清生化指标。[结果] 植物提取物组奶牛干物质采食量降低0.51 kg/d,产奶量提高0.85 kg/d（P>0.05）。植物提取物组血清甘油三酯与对照组相比降低45.6%（P<0.05）。与对照组相比,植物提取物组血清催乳素和生长激素显著提高（P<0.05）。[结论] 日粮中添加20 g/d茶多酚和植物甾醇复合物能够提高奶牛日粮产奶效率,改善奶牛脂肪代谢过程中的问题,提高生产性能。
[Objective]To study the biological functions of tea polyphenols and phytosterol complexes and their effects on feed intake,milk production performance and serum biochemical indicators of mid-to-late lactating cows. [Method]Sixty healthy cows with similar age,parity,lactation time and milk yield were randomly divided into 2 groups,with 30 cows in each group. The control group was fed a basic diet,and the experimental group was fed a basal diet supplemented with 20 g/d plant extracts. The pre-trial period lasted for 7 days and the experimental period lasted for 70 days. Milk and blood samples were collected and milk composition and serum biochemical indicators were determined. [Result]Dry matter intake decreased by 0.51 kg/d and milk yield increased by 0.85 kg/d in experimental group（P>0.05）. Serum triglycerides in experimental group decreased by 45.6% compared with control group（P<0.05）. Compared with control group,serum prolactin and growth hormone in experimental group were significantly increased（P<0.05）. [Conclusion]Supplementing 20 g/d tea polyphenols and phytosterol complexes can improve milk production efficiency,fat metabolism and productivity of cows.
[1] 江滢,沈思婷,夏如枫,等. 茶多酚的化学组成及其含量测定与结构鉴定[J]. 机电信息,2018(20):1-9.
[2] Vuong Q V,Stathopoulos C E,Nguyen M H,et al.Isolation of green tea catechins and their utilization in the food industry[J]. Food Reviews International,2011,27(3):227-247.
[3] Huang J B,Zhang Y,Zhou Y B,et al.Effects of epigallocatechin gallate on lipid metabolism and its underlying molecular mechanism in broiler chickens[J]. Journal of Animal Physiology and Animal Nutrition,2015,99(4):719-727.
[4] 贾红豆. 茶多酚通过NF-κB通路缓解LPS诱导的奶牛乳腺上皮细胞炎性反应[J]. 中国兽医学报,2021,41(7):1372-1375.
[5] Phillips K M,Ruggio D M,Toivo J I,et al.Free and esterified sterol composition of edible oils and fats[J]. Journal of Food Composition and Analysis,2002,15(2):123-142.
[6] Ortiz-Escarzartiz-Escarza J M,Medina,M E, Trigos A. On the peroxyl radical scavenging ability of β-sitosterol in lipid media:A theoretical study[J]. Journal of Physical Organic Chemistry,2021,34(1):1-8.
[7] Goff M L,Ferrec E L,Mayer C,et al.Microalgal carotenoids and phytosterols regulate biochemical mechanisms involved in human health and disease prevention[J]. Biochimie, 2019,167:106-118.
[8] Shi S R,Shen Y R, Chang L L,et al.Safety evaluation of phytosterols in laying hens:Effects on laying performance,clinical blood parameters,and organ development[J]. Poultry Science, 2014,93(3):545-549.
[9] Feng S M,Dai Z Q,Liu A B,et al.Intake of stigmasterol and β-sitosterol alters lipid metabolism and alleviates NAFLD in mice fed a high-fat western-style diet[J]. Biochimica Et Biophysica Acta-Molecular and Cell Biology of Lipids,2018,1863(10):1274-1284.
[10] 齐云霞,吴涛,胡晓苗,等. 茶多酚对围产后期奶牛生产性能、血清生化及抗氧化指标的影响[J]. 中国畜牧兽医,2023,50(10):4070-4076.
[11] 刘冬,丁兆忠,吴忠良,等. 不同水平的茶多酚对育肥猪生长性能、肉品质及抗氧化功能的影响[J]. 中国饲料,2021,(15):48-53.
[12] 谢心美,郝海鑫,何剑斌. 植物甾醇对泌乳前期奶牛生产性能及血浆生化指标的影响[J]. 现代畜牧兽医,2013(9):16-22.
[13] 雷霆. 植物甾醇对育肥猪生长性能、肉品质和血清生化指标的影响[D]. 长沙: 湖南农业大学,2022.
[14] 梁辛,李孟伟,杨承剑,等. 植物甾醇对泌乳水牛产奶量及乳品质的影响[J]. 饲料研究,2017(16):42-45,52.
[15] Gessner,D K,Brock C,Hof L M, et al. Effects of supplementation of green tea extract on the milk performance of peripartal dairy cows and the expression of stress response genes in the liver[J]. Journal of Animal Science and Biotechnology,2020,11(4):1163-1174.
[16] 屈金涛. 茶多酚对奶山羊生产性能、消化特性和血液指标的影响[D]. 长沙:湖南农业大学,2022.
[17] 吕润全,周国波,陈昊,等. 植物甾醇对泌乳前期奶牛产奶量及乳成分的影响[J]. 畜牧与兽医,2010,42(12):13-16.
[18] 谢颖,金志红,朱靖,等. 植物甾醇对奶牛生产性能、血液胆固醇和抗氧化能力的影响[J]. 中国奶牛,2020(2):12-18.
[19] 王冬凯,费兆生. 茶叶提取物对奶牛生产性能及血液抗氧化指标的影响[J]. 中国奶牛,2014(7):33-36.
[20] 梁遵山. 荷斯坦奶牛乳汁SCC与肝功酶、抗氧化及酮体的关系[D]. 南宁:广西大学,2018.
[21] 肖红艳,屈金涛,凌浩,等. 茶多酚对奶山羊生产性能、血液指标和抗氧化功能的影响[J]. 动物营养学报,2021,33(8):4533-4540.
[22] 扈添琴. 日粮中添加不同水平酶制剂与植物甾醇复合物对奶牛生产性能和血液指标的影响[D]. 南京:南京农业大学,2014.
[23] 闫碧川,李振乾,李胜利,等. 不同酵母培养物对泌乳中后期奶牛生产性能、养分表观消化率以及血清指标的影响[J]. 动物营养学报,2018,30(7):2732-2740.
[24] 夏英姿,于振洋. 不同水平酯化植物甾醇对仔猪生长性能、免疫功能及血清生化指标的影响[J]. 饲料研究,2023,46(3):26-29.
[25] 张翼,刘继根,曹娜,等. 植物甾醇和胍基乙酸复合物对育肥肉牛生长性能、屠宰性能、胴体品质和血清生化指标的影响[J]. 饲料博览,2022(6):31-37.
[26] 柴晓杰,余涛,王淑萍. 奶牛血浆激素水平与产奶性能的关系[J]. 吉林农业大学学报, 1999(S1):74-76.
[27] Freeman M E,Kanyicska B,Anna L,et al.Prolactin: Structure,function,and regulation of secretion[J]. Physiological Reviews,2000,80(4):1523-1631.
[28] Chung E R,Rhim T J,Han S K.Associations between PCR-RFLP markers of growth hormone and prolactin genes and production traits in dairy cattle[J]. Korean Journal of Animal Science,1996,38(4):321-336.
[29] 扈添琴,韩兆玉,王群,等. 酶制剂和植物甾醇复合物对泌乳奶牛生产性能和血清指标的影响[J]. 动物营养学报,2014,26(1):236-244.
[30] Jiang H,Ge X.MEAT SCIENCE AND MUSCLE BIOLOGY SYMPOSIUM-Mechanism of growth hormone stimulation of skeletal muscle growth in cattle[J]. Journal of Animal Science,2014,92(1):21-29.
[31] 孙伟男. 獭兔生长激素基因克隆及原核细胞表达的初步研究[D]. 呼和浩特:内蒙古农业大学,2013.
[32] 卓锐文,陈罡,杨凯丽,等.饲粮中添加酯化植物甾醇对断奶仔猪生长性能,养分表观消化率,血清生化指标,回肠消化酶活性及肠道免疫指标的影响[J].动物营养学报, 2022, 34(7):4237-4248.
[33] 金志红. 植物甾醇在奶牛上的应用及其机理初探[D]. 南京:南京农业大学,2012.
