[目的] 评价牛初乳益生菌粉调节免疫和肠道菌群保健功能。[方法] （1）有助于增强免疫力功能评价。分别以0.415 g/kg·BW、0.830 g/kg·BW、1.66 g/kg·BW三个剂量的牛初乳益生菌粉（相当于人体推荐剂量的5倍、10倍、20倍）连续给小鼠灌胃30 天后,测定细胞免疫功能、体液免疫功能、单核-巨噬细胞功能和NK细胞活性。（2）有助于调节肠道菌群功能评价。分别以0.415 g/kg·BW、0.830 g/kg·BW、1.245 g/kg·BW三个剂量的牛初乳益生菌粉（相当于人体推荐剂量的5倍、10倍、15倍）连续给小鼠灌胃14 天后,观察试验前后自身及组间肠道菌群（肠球菌、肠杆菌、产气荚膜梭菌、乳杆菌、双歧杆菌）变化情况。[结果] （1）0.830 g/kg·BW、1.660 g/kg·BW剂量的牛初乳益生菌粉可明显增强小鼠的迟发型变态反应（P<0.05或P<0.01）,1.660 g/kg·BW剂量的牛初乳益生菌粉可提高小鼠的半数溶血值（HC₅₀）（P<0.01）,0.830 g/kg·BW、1.660 g/kg·BW剂量的牛初乳益生菌粉可增强腹腔巨噬细胞的吞噬功能（P<0.05或P<0.01）。（2）与对照组以及自身灌胃前相比,各剂量组产气荚膜梭菌、肠杆菌、肠球菌含量无显著差异（P>0.05）。0.830 g/kg·BW、1.245 g/kg·BW剂量组双歧杆菌和乳杆菌含量明显增加,差异有显著性（P<0.05或P<0.01）。[结论] 牛初乳益生菌粉有增强免疫力、调节肠道菌群的保健功能。

[Objective]Evaluation of the immune-regulating and gut microbiota health-care functions of bovine colostrum probiotic powder.[Method]（1）Helps to enhance immune function evaluation. After continuous gavage of bovine colostrum probiotic powder at three doses of 0.415 g/kg·BW, 0.830 g/kg·BW, and 1.660 g/kg·BW （equivalent to 5, 10, and 20 times the recommended human dose） to mice for 30 days,cellular immune function,humoral immune function,monocyte macrophage function and NK cell activity were determined.（2）Helps to regulate gut microbiota function evaluation. After continuous gavage of bovine colostrum probiotic powder at three doses of 0.415 g/kg·BW, 0.830 g/kg·BW, and 1.245 g/kg·BW （equivalent to 5, 10, and 15 times the recommended human dose） to mice for 14 days,the changes were observed in gut microbiota（Enterococcus,Escherichia coli,Clostridium perfringens,Lactobacillus,Bifidobacterium） before and after the experiment,both within and between groups.[Result]（1）The bovine colostrum probiotic powder at doses of 0.830 g/kg·BW and 1.660 g/kg·BW could significantly enhance delayed type hypersensitivity in mice（P<0.05 or P<0.01）,the bovine colostrum probiotic powder at doses of 1.660 g/kg·BW could increase the half hemolysis value（HC₅₀） of mice（P<0.01）,and the bovine colostrum probiotic powder at doses of 0.830 g/kg·BW and 1.66g/kg·BW could enhance the phagocytic function of peritoneal macrophages（P<0.05 or P<0.01）.（2）Compared with the control group and before self gavage,there were no significant difference in the content of Clostridium perfringens,Escherichia coli,and Enterococcus in each dose group（P>0.05）.The content of Bifidobacterium and Lactobacillus increased significantly in the 0.830 g/kg·BW and 1.245 g/kg·BW dose groups,with significant differences（P<0.05 or P<0.01）.[Conclusion]The bovine colostrum probiotic powder had the health functions of enhancing immunity and regulating gut microbiota.