[目的] 口蹄疫危害性不容忽视,监测免疫抗体效价是了解牛群抗病力的最有效途径,可帮助奶牛场评估免疫效果,为防控口蹄疫提供权威和科学依据。[方法] 随机抽取合肥地区5 个规模奶牛场,各采集40 份血清样品,采用阻断ELISA试验对不同日龄的牛（犊牛、育成牛、青年牛、成年牛）血清中口蹄疫抗体水平监测和分析。[结果] 大部分场点达到农业农村部规定的70%合格标准。[结论] 合肥地区规模奶牛场使用较多的是口蹄疫O型-A型二价灭活疫苗,免疫效果良好,可及时产生有效抗体。定期监测奶牛口蹄疫抗体水平,是评估免疫接种效果的有效方法,同时为制定完善的免疫规划,加强防控措施,实施科学的预警和预报机制,最大限度减少口蹄疫疫情发生提供数据参考。

[Objective] The harm of FMD disease cannot be ignored. Monitoring the immune antibody titer is the most effective way to understand the epidemic dynamics, which can help the pastures to evaluate the immune effect, and provide authoritative and scientific basis for the prevention and control of FMD disease. [Method] Five large-scale dairy farms were randomly selected in Hefei and 40 serum samples were collected each. FMD antibody levels in serum of cattle（calves, bred cattle, young cattle, and adult cattle）were monitored and analyzed by blocking ELISA test. [Result] Most of the sites reached the 70% qualification standard of the Ministry of Agriculture and Rural Affairs. [Conclusion] FMD disease OA bivalent vaccine is widely used in large-scale dairy farms in Hefei, which has good immune effect and can produce effective antibodies in time. Regular monitoring of the antibody level of FMD disease in dairy cows is an effective method to evaluate the effect of immunization, and provides data reference for formulating a perfect immunization plan, strengthening prevention and control measures, implementing scientific early warning and forecast mechanism, and minimizing the occurrence of FMD disease.