假单胞菌属是生乳中最常见的嗜冷菌属之一,为探讨影响假单胞菌属动力学参数的主要因素,本研究以定量整合微生物动力学参数并量化菌株变异性的方法开展荟萃分析。通过统计750 余条假单胞菌属在不同条件下的生长参数,并基于平方根模型预测其最低生长温度（T_min）,结果表明,假单胞菌属整体T_min为-4.06 ℃,其中菌株1248和E52在培养基中的T_min分别为-5.38 ℃和-6.36 ℃。在低温环境下,UHT牛乳中高初始接菌量（Y₀）显著缩短了迟滞期（λ）,使微生物即使在较低温度下也能更快进入对数生长期。在温度（T）、pH值、水分活度（Aw）和NaCl浓度的共同作用下,最大比生长速率（μ_max）分布范围为0.04~1.63 h^-1。此外,NaCl浓度（1.0%~7.5%）对假单胞菌属的生长具有显著抑制作用。本研究可为乳及乳制品生产过程中的安全性、生产优化和质量控制提供重要参考依据。

Pseudomonas spp. is one of the most common genera of cryophilic bacteria in raw milk,and in order to explore the main factors affecting the kinetic parameters of Pseudomonas spp.,this study carried out a meta-analysis by quantitatively integrating the kinetic parameters of microorganisms and quantifying the variability of the strains.By statistically analyzing the growth parameters of more than 750 Pseudomonas spp. under different conditions and predicting their minimum growth temperatures（Tmin） based on the square root model,the results showed that the overall Tmin value of Pseudomonas spp. was -4.06 ℃,with the Tmin of strains 1248 and E52 in the culture medium being -5.38 ℃ and -6.36 ℃,respectively.In a low-temperature environment,the high initial inoculum size （Y₀,log CFU/mL） in UHT milk significantly shortened the growth retardation period,allowing the microorganisms to enter the logarithmic growth period（λ） more quickly even at lower temperatures.The distribution of maximum specific growth rate（μmax） under the combined effect of temperature,pH value, activity water（Aw） and NaCl concentration ranged from 0.04 h^-1 to 1.63 h^-1.In addition,NaCl concentration（1.0% to 7.5%） had a significant inhibitory effect on the growth of Pseudomonas spp..This study provided important reference basis for the safety,production optimisation and quality control during the production of milk and dairy products.