[目的]为了解山东省聊城市奶山羊乳腺炎金黄色葡萄球菌（S.aureus）流行现状及其耐药基因分布情况。[方法]采集山东省聊城市部分奶山羊场178 份乳样,通过细菌培养、血浆凝固酶试验和PCR生物学技术进行分离鉴定S.aureus,分析分离菌株耐药性。[结果]鉴定出23 株S.aureus,检出率为12.92%;分离菌株对青霉素、强力霉素、链霉素、克拉霉素和四环素耐药性较强;耐药基因PCR检测发现,10 种耐药基因均有检出。其中,β-内酰胺类耐药基因mecA,大环内酯类耐药基因ermA、ermB、ermC,四环素类耐药基因tet,氨基糖苷类耐药基因aac（6'）-Ⅰa、aph（2''）-Ⅰb、ant（4',4''）、aph（3'）-II,磺胺类耐药基因sul1平均携带率分别为30.43%、31.88%、43.48%、21.73%、8.70%,所有菌株至少携带2 种耐药基因,最多7 种耐药基因,以携带4~5 种耐药基因菌株数量最多。[结论]山东省聊城市奶山羊场存在S.aureus流行传播风险,且菌株表现不同程度的耐药性,耐药基因携带率较高,应引起重视,加强监测和防控。
[Objective] In order to understand the distribution of Staphylococcus aureus（S.aureus） and its drug resistance genes from dairy goat mastitis. [Method] 178 milk samples were collected from some dairy goat farms in Liaocheng city,Shandong Province,and S.aureus was isolated and identified by bacterial culture,plasma coagulase test and PCR biological technology,and the drug resistance of the isolated strains was further analyzed. [Result] 23 strains of S.aureus were identified,and the detection rate was 12.92%.The results of drug sensitivity test showed that the isolated strains were resistant to penicillin,doxycycline,streptomycin,clarithromycin and tetracycline.PCR detection of drug resistance genes showed that 10 kinds of drug resistance genes were detected,including β-lactam drug resistance gene mecA,macrolide drug resistance genes ermA,ermB,ermC,tetracycline drug resistance gene tet,aminoglycoside drug resistance genes AAC（6'）-1A,APH（2''）-1B,ant（4',4''）.β-lactam resistance gene mecA, macrolide resistance genes ermA, ermB, ermC, tetracycline resistance gene tet, aminoglycoside resistance genes aac（6'）-Ⅰa, aph（2''）-Ⅰb, ant（4',4''）, aph（3'）-III, and sulfonamide resistance gene sul1 had average carriage rates of 30.43%, 31.88%, 43.48%, 21.73%, and 8.70%, respectively.All strains carried at least 2 kinds of drug resistance genes and up to 7 kinds of drug resistance genes,with the largest number of strains carrying 4~5 kinds of drug resistance genes. [Conclusion] The results suggested that there was a risk of S.aureus epidemic transmission in Liaocheng city dairy goat farms,Shandong Province,and the strains showed different degrees of drug resistance,and the drug resistance gene carrying rate was high,which should be paid attention to and strengthened monitoring and prevention.
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