[目的]探讨中草药提取物对奶牛隐性乳腺炎的防控效果。[方法]选取24 头隐性乳腺炎奶牛,随机分为4 组。对照组给予正常饲料,试验组分别饲喂20 g/d、40 g/d和80 g/d的中草药提取物。记录各组奶牛产奶量、奶质量,以及牛髓过氧化物酶（MPO）、乳酸脱氢酶（LDH）、谷胱甘肽过氧化物酶（GPX）、超氧化物歧化酶（SOD）、过氧化氢酶（CAT）、丙二醛（MDA）、白介素-6（IL-6）、白介素-1β（IL-1β）和肿瘤坏死因子-α（TNF-α）的含量变化。[结果]饲喂40 g/d和80 g/d中草药提取物的奶牛,其产奶量、乳糖率、乳蛋白率显著提高,体细胞数显著减少,同时MPO和LDH的含量下降。此外,GSH、SOD和CAT活性增强,MDA含量降低,炎症因子IL-6、IL-1β和TNF-α的表达显著抑制。[结论]中草药提取物能够有效提高隐性乳腺炎奶牛的产奶量和奶质量,调节乳中活性酶含量,增强抗氧化能力,抑制炎症因子的表达,有良好的防控效果。
[Objective] This study aimed to investigate the preventive and therapeutic effects of Chinese herbal medicine extract （CHME） on subclinical mastitis in dairy cows.[Method] Twenty-four dairy cows with subclinical mastitis were randomly divided into four groups.The control group received a standard diet,while the three treatment groups were supplemented with CHME at 20 g/d,40 g/d,and 80 g/d,respectively.Milk yield,milk composition （including lactose percentage,protein percentage,and somatic cell count）, and the levels of key biomarkers were recorded. These biomarkers included myeloperoxidase（MPO）,lactate dehydrogenase（LDH）,glutathione peroxidase （GPX）,superoxide dismutase （SOD）,catalase（CAT）, malondialdehyde （MDA）,interleukin-6 （IL-6）,interleukin-1β （IL-1β）,and tumor necrosis factor-α （TNF-α）.[Result] Cows supplemented with 40 g/d and 80 g/d of CHME exhibited a significant increase in milk yield,lactose percentage,and protein percentage,along with a significant reduction in somatic cell count. Concurrently,the levels of MPO and LDH decreased.Furthermore,the activities of the antioxidant enzymes GPX,SOD,and CAT were enhanced,while MDA content decreased. The expression of the pro-inflammatory cytokines IL-6,IL-1β,and TNF-α was significantly suppressed.[Conclusion] Chinese herbal medicine extract effectively improved milk yield and quality in cows with subclinical mastitis.It regulated the levels of active enzymes in milk,enhanced antioxidant capacity,and inhibited the expression of inflammatory cytokines,demonstrating good preventive and therapeutic effects.
[1] 王天硕,仝静迪,赵岩,等.奶牛金黄色葡萄球菌性乳房炎及预防措施[J].今日畜牧兽医,2024,40(12):39-41.
[2] 徐鸿润,刘沛尧,邢小光,等.益生菌及其替代抗生素治疗奶牛乳腺炎研究进展[J].中国奶牛,2021(10):1-4.
[3] 韩俊伟,杜海燕.基于数据挖掘的中药治疗奶牛乳房炎用药规律分析[J].中国动物保健,2024,26(10):50-51,73.
[4] 彭清洁,蔡大亮,姜鹏,等.蒲公英提取物及其复合酵母培养物对奶牛隐形乳房炎的药效评价[J].中国饲料,2024(5):166-171.
[5] 魏秀丽,李晴,徐恩民,等.蒲公英提取物联合多种抗生素对奶牛乳房炎金黄色葡萄球菌的体外协同抑菌实验[J].中国兽药杂志,2020,54(4):70-76.
[6] 聂德超,张卓,赵琛,等.黄芩苷的生物学功能及在奶牛上的应用研究进展[J].动物营养学报,2022,34(1):83-92.
[7] 李吉鹏,路强.金银花提取物对奶牛生产性能、乳品质及血清免疫指标的影响[J].中国饲料,2023(16):13-16.
[8] 周艳,张涛,郑勤琴,等.连翘有效成分提取及提取物对奶牛乳房炎致病菌的抑制效果[J].生物技术进展,2014,4(4):292-295,305.
[9] 吕平,韦丽君,黄强,等.中草药提取物与酶复合物对牛乳房炎病原菌的抑菌效果[J].西北农业学报,2012,21(3):32-37.
[10] 潘佳亮,刘平平,魏成威,等.奶牛隐性乳房炎敏感性中草药组方随机区组筛选试验[J]. 黑龙江畜牧兽医,2012,4(7):129-131.
[11] 郝景锋,李静姬,张宇航,等.20 味中药对奶牛隐性乳房炎3 种病原菌体外抑菌效果比较[J].中国兽医杂志,2017,53(2):55-58.
[12] 钟华晨,王丽芳,张三粉,等.复合植物水提物对奶牛乳房炎致病菌的体外抑制效果[J].饲料工业,2020,41(18):17-22.
[13] Jia P,Tu Y,Liu Z,et al.Diets supplementation with Bacillus subtilis and Macleaya cordata extract improve production performance and the metabolism of energy and nitrogen,while reduce enteric methane emissions in dairy cows[J].Animal Feed Science and Technology,2022,294:115481.
[14] 田猛,李纯锦,易康乐,等.复方发酵中草药对奶牛隐性乳腺炎的预防和治疗[J].黑龙江动物繁殖,2020,28(1):4-8.
[15] 王飒爽,武嘉平,刘倩,等.发酵中药添加剂对奶牛乳腺炎预防效果及其产奶性能的影响研究[J].饲料研究,2019,42(7): 10-12.
[16] 杨德英. 奶牛乳房炎对乳酶活性、急性期蛋白含量的影响及其作为隐性乳房炎诊断指标的研究[D].成都:四川农业大学,2009.
[17] Depreester E,Meyer E,Demeyere K,et al.Flow cytometric assessment of myeloperoxidase in bovine blood neutrophils and monocytes[J].Journal of Dairy Science,2017,100(9):7638-7647.
[18] 孙雅望. 脂多糖引起奶牛乳腺和机体氧化应激损伤及蒲公英提取物对其阻断效果的研究[D].重庆:西南大学,2021.
[19] 栗福星. 不同水平中草药添加剂对泌乳期奶牛泌乳性能、血清生化、免疫指标及抗氧化功能的影响[J].饲料研究,2022,45(9):18-21.
[20] Zhang J,Zhang Y,Huang H,et al.Forsythoside a inhibited S. aureus stimulated inflammatory response in primary bovine mammary epithelial cells[J].Microb Pathog,2018,116:158-163.
[21] Yang W,Li H,Cong X,et al.Baicalin attenuates lipopolysaccharide induced inflammation and apoptosis of cow mammary epithelial cells by regulating NF-kappaB and HSP72[J].International Immunopharmacology,2016,40:139-145.
