疾病防治

金黄色葡萄球菌引起牛乳腺炎的抗微生物应对措施

  • 严海涛 ,
  • 纪双慧 ,
  • 胡智胜
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  • 1 山东省日照市莒县夏庄畜牧兽医站,山东日照 276500;
    2 山东省日照市莒县畜牧兽医管理服务中心,山东日照 276500;
    3 山东省畜牧总站,山东济南 250102
严海涛(1984-),男,山东日照人,本科,高级兽医师,研究方向为兽医;纪双慧(1982-),女,山东青岛人,本科,兽医师,研究方向为动物医学。

网络出版日期: 2025-09-10

基金资助

2023年山东省重点研发计划(农业良种工程品种培育)项目-奶牛关键性状智能选育技术研究与应用(2023LZGC004)

The Antimicrobial Strategies against Staphylococcus aureus-induced Bovine Mastitis

  • YAN Haitao ,
  • JI Shuanghui ,
  • HU Zhisheng
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  • 1 Xiazhuang Animal Husbandry and Veterinary Hospital,Ju County,Rizhao Shandong 276500;
    2 Ju County Animal Husbandry and Veterinary Management Service Center,Rizhao Shandong 276500;
    3 Shandong Animal Husbandry Station,Jinan Shandong 250102

Online published: 2025-09-10

摘要

乳腺炎是全球范围内影响奶牛的常见疾病,主要由金黄色葡萄球菌引起,通常依赖乳腺内注射和肌肉注射抗微生物药物治疗,但随着抗微生物耐药性的上升,治疗面临更大挑战且可选方案逐渐减少。生物膜的形成、致病菌在乳腺上皮细胞中的附着,使治疗更复杂。本文综述抗微生物耐药性增加、生物膜形成和细菌在细胞内存活等因素,分析常规抗生素治疗乳腺炎的挑战。同时,介绍植物化学化合物、抗微生物肽、噬菌体治疗及基于石墨烯纳米材料的替代治疗方法,这些方法有望弥补现有抗微生物药物治疗的不足,缓解抗微生物耐药性对乳腺炎治疗的威胁。本文旨在探讨乳腺炎治疗中的新兴替代方案,以期为未来的临床治疗提供有力支持。

本文引用格式

严海涛 , 纪双慧 , 胡智胜 . 金黄色葡萄球菌引起牛乳腺炎的抗微生物应对措施[J]. 中国乳业, 2025 , 0(8) : 67 -74 . DOI: 10.12377/1671-4393.25.08.11

Abstract

Mastitis is a common disease affecting dairy cows globally,primarily caused by Staphylococcus aureus,and is typically treated with antimicrobial drugs administered via intramammary and intramuscular injections.However,with the increasing rise of antimicrobial resistance,the treatment faces greater challenges and the available therapeutic options are gradually diminishing.The formation of biofilms formation and the adhesion of pathogens to mammary epithelial cells complicate treatment further.This paper discusseed factors such as the rise in antimicrobial resistance,biofilm formation,and intracellular bacterial survival,analyzing the challenges of conventional antibiotic treatment for mastitis.Additionally,it introduces alternative therapeutic approaches,including phytochemicals,antimicrobial peptides,bacteriophage therapy,and graphene-based nanomaterials,which may address the shortcomings of current antimicrobial treatments and mitigate the threat of antimicrobial resistance to mastitis treatment.This paper aimed to explore emerging alternative approaches in the treatment of mastitis,with the goal of providing robust support for future clinical therapies.

参考文献

[1] Zhao C,Bao L,Qiu M,et al.Commensal cow Roseburia reduces gut-dysbiosis-induced mastitis through inhibiting bacterial translocation by producing butyrate in mice[J].Cell Reports,2022,41(8):111681.
[2] El-Sayed A,Kamel M.Bovine mastitis prevention and control in the post-antibiotic era[J].Tropical Animal Health and Production,2021,53(2):236.
[3] Cheng W N, Han S G.Bovine mastitis:Risk factors,therapeutic strategies,and alternative treatments - A review[J].Asian Australasian Journal of Animal Sciences,2020,33(11):1699-1713.
[4] Borena B M,Gurmessa F T,Gebremedhin E Z,et al.Staphylococcus aureus in cow milk and milk products in Ambo and Bako towns,Oromia,Ethiopia:Prevalence,associated risk factors,hygienic quality,and antibiogram[J].International Microbiology,2023,26(3):513-527.
[5] Cui M,Li J,Ali T,et al.Emergence of livestock-associated MRSA ST398 from bulk tank milk,China[J].Journal of Antimicrobial Chemotherapy,2020,75(12):3471-3474.
[6] Sharun K, Dhama K, Tiwari R, Gugjoo MB, Iqbal Yatoo M, Patel SK, Pathak M, Karthik K, Khurana SK, Singh R, Puvvala B, Amarpal, Singh R, Singh KP, Chaicumpa W. Advances in therapeutic and managemental approaches of bovine mastitis: a comprehensive review[J]. Veterinary Quarterly,2021,41(1):107-136.
[7] Lin X Q,Liu Z Z,Zhou C K,et al.Trained immunity in recurrent Staphylococcus aureus infection promotes bacterial persistence[J].PLoS Pathog,2024,20(1):e1011918.
[8] Cameron A,Mcallister T A.Antimicrobial usage and resistance in beef production[J].Journal of Animal Science and Biotechnology,2016,7:68.
[9] Sharun K,Dhama K,Tiwari R,et al.Advances in therapeutic and managemental approaches of bovine mastitis:A comprehensive review[J].Veterinary Quarterly,2021,41(1):107-136.
[10] Aggarwal R,Mahajan P,Pandiya S,et al.Antibiotic resistance:A global crisis,problems and solutions[J].Critical Reviews in Microbiology,2024,50(5):896-921.
[11] Sharifi A,Sobhani K,Mahmoudi P.A systematic review and meta-analysis revealed a high-level antibiotic resistance of bovine mastitis Staphylococcus aureus in Iran[J].Research in Veterinary Science,2023,161:23-30.
[12] Wang K,Cha J,Liu K,et al.The prevalence of bovine mastitis-associated Staphylococcus aureus in China and its antimicrobial resistance rate:A meta-analysis[J].Frontiers in Veterinary Science,2022,9:1006676.
[13] Kwiecinski J M,Horswill A R.Staphylococcus aureus bloodstream infections:Pathogenesis and regulatory mechanisms[J].Current Opinion in Microbiology,2020,53:51-60.
[14] Molineri A I,Camussone C,Zbrun M V,et al.Antimicrobial resistance of Staphylococcus aureus isolated from bovine mastitis:Systematic review and meta-analysis[J].Preventive Veterinary Medicine,2021,188:105261.
[15] Chehabi C N,Nonnemann B,Astrup L B,et al.In vitro antimicrobial resistance of Causative Agents to clinical mastitis in Danish dairy cows[J].Foodborne Pathogens and Disease,2019,16(8):562-572.
[16] Saeed S I,Mat Yazid K A,Hashimy H A,et al.Prevalence,antimicrobial resistance,and characterization of Staphylococcus aureus isolated from subclinical bovine mastitis in East Coast Malaysia[J].Animals(Basel),2022,12(13):1680.
[17] Tran M T,Vu D M,Vu M D,et al.Antimicrobial resistance and molecular characterization of Klebsiella species causing bovine mastitis in Nghe An Province,Vietnam[J].Journal of Advanced Veterinary & Animal Research,2023,10(1):132-143.
[18] Jasińska J M,Kamińska I,Chmiel M J,et al.Biological potential of polysaccharides extracted from Nostoc colonies for film production - Physical and biological properties[J].Biotechnology Journal,2023,18(5):e2200455.
[19] Tan L F,Elaine E,Pui L P,et al.Development of chitosan edible film incorporated with Chrysanthemum morifolium essential oil[J].Acta Scientiarum Polonorum Technologia Alimentaria,2021,20(1):55-66.
[20] Zaatout N,Ayachi A,Kecha M.Staphylococcus aureus persistence properties associated with bovine mastitis and alternative therapeutic modalities[J].Journal of Applied Microbiology,2020,129(5):1102-1119.
[21] Feng W,Chittò M,Moriarty T F,et al.Targeted drug delivery systems for eliminating intracellular bacteria[J].Macromolecular Bioscience,2023,23(1):e2200311.
[22] Kamaruzzaman N F,Kendall S,Good L.Targeting the hard to reach:Challenges and novel strategies in the treatment of intracellular bacterial infections[J].British Journal of Pharmacology,2017,174(14):2225-2236.
[23] Leon-Sicairos N,Reyes-Cortes R,Guadrón-Llanos A M,et al.Strategies of intracellular pathogens for obtaining iron from the environment[J].Biomed Research International,2015,2015:476534.
[24] Disson O,Moura A,Lecuit M.Making sense of the biodiversity and virulence of Listeria monocytogenes[J].Trends in Microbiology,2021,29(9):811-822.
[25] Baranyai Z,Krátký M,Vosátka R,et al.In vitro biological evaluation of new antimycobacterial salicylanilide-tuftsin conjugates[J].European Journal of Medicinal Chemistry,2017,133:152-173.
[26] Sánchez M,González-Burgos E,Iglesias I,et al.Pharmacological update properties of Aloe Vera and its major active constituents[J].Molecules,2020,25(6):1324.
[27] Shin B,Park W.Zoonotic diseases and phytochemical medicines for microbial infections in veterinary science:Current state and future perspective[J].Frontiers in Veterinary Science,2018,5:166.
[28] Dogra S,Koul B,Singh J,et al.Phytochemical analysis,antimicrobial screening and in vitro pharmacological activity of Artemisia vestita leaf extract[J].Molecules,2024,29(8):1829.
[29] Srichok J,Yingbun N,Kowawisetsut T,et al.Synergistic antibacterial and anti-inflammatory activities of Ocimum tenuiflorum ethanolic extract against major bacterial mastitis pathogens[J].Antibiotics(Basel),2022,11(4):510.
[30] Hase P,Digraskar S,Ravikanth K,et al.Management of subclinical mastitis with mastilep gel and herbal spray(AV/AMS/15)[J].International Journal of Pharmacy and Pharmacology,2013,2(4):64-67.
[31] Cordeiro L,Figueiredo P,Souza H,et al.Terpinen-4-ol as an antibacterial and antibiofilm agent against Staphylococcus aureus[J].International Journal of Molecular Sciences,2020,21(12):4531.
[32] Deo S,Turton K L,Kainth T,et al.Strategies for improving antimicrobial peptide production[J].Biotechnology Advances,2022,59:107968.
[33] Mhlongo J T,Waddad A Y,Albericio F,et al.Antimicrobial peptide synergies for fighting infectious diseases[J].Advanced Science,2023,10(26):e2300472.
[34] Tomasinsig L,De Conti G,Skerlavaj B,et al.Broad-spectrum activity against bacterial mastitis pathogens and activation of mammary epithelial cells support a protective role of neutrophil cathelicidins in bovine mastitis[J].Infection and Immunity,2010,78(4):1781-1788.
[35] Shah P,Shrivastava S,Singh R J,et al.Synthetic antimicrobial peptide Polybia MP-1(Mastoparan) inhibits growth of antibiotic resistant Pseudomonas aeruginosa isolates from mastitic cow milk[J].International Journal of Peptide Research and Therapeutics,2021,27(4):2471-86.
[36] Cao L T,Wu J Q,Xie F,et al.Efficacy of nisin in treatment of clinical mastitis in lactating dairy cows[J].Journal of Dairy Science,2007,90(8):3980-5.
[37] Dion M B,Oechslin F,Moineau S.Phage diversity,genomics and phylogeny[J].Nature Reviews Microbiology,2020,18(3):125-138.
[38] Salmond G P,Fineran P C.A century of the phage:Past,present and future[J].Nature Reviews Microbiology,2015,13(12):777-786.
[39] Piel D,Bruto M,Labreuche Y,et al.Phage-host coevolution in natural populations[J].Nat Microbiol,2022,7(7):1075-1086.
[40] Teng F,Xiong X,Zhang S,et al.Efficacy assessment of phage therapy in treating Staphylococcus aureus-induced mastitis in mice[J].Viruses,2022,14(3):620.
[41] Guo M,Gao Y,Xue Y,et al.Bacteriophage cocktails protect dairy cows against mastitis caused by drug resistant Escherichia coli infection[J].Frontiers in Cellular and Infection Microbiology,2021,11:690377.
[42] Ngassam-Tchamba C,Duprez J N,Fergestad M,et al.In vitro and in vivo assessment of phage therapy against Staphylococcus aureus causing bovine mastitis[J].Journal of Global Antimicrobial Resistance,2020,22:762-770.
[43] Geng H,Zou W,Zhang M,et al.Evaluation of phage therapy in the treatment of Staphylococcus aureus-induced mastitis in mice[J]. Folia Microbiologica,2020,65(2):339-351.
[44] Allahbakhsh A,Gadegaard N,Ruiz C M,et al.Graphene-based engineered living materials[J].Small Methods,2024,8(1):e2300930.
[45] Jeong W Y,Choi H E,Kim K S.Graphene-based nanomaterials as drug delivery carriers[J].Advances in Experimental Medicine and Biology,2022,1351:109-124.
[46] Vakili B,Karami-Darehnaranji M,Mirzaei E,et al.Graphene oxide as novel vaccine adjuvant[J].International Immunopharmacology,2023,125(Pt A):111062.
[47] Saeed S I,Vivian L,Zalati C W S C W,et al.Antimicrobial activities of graphene oxide against biofilm and intracellular Staphylococcus aureus isolated from bovine mastitis[J].BMC Veterinary Research,2023,19(1):10.
[48] Yang S,Baeg E,Kim K,et al.Neurodiagnostic and neurotherapeutic potential of graphene nanomaterials[J].Biosensors and Bioelectronics,2024,247:115906.
[49] Xu Y,Wang Y,He J,et al.Antibacterial properties of lactoferrin:A bibliometric analysis from 2000 to early 2022[J].Front Microbiol,2022,13:947102.
[50] Kurchenko V,Halavach T,Yantsevich A,et al.Chitosan and its derivatives regulate lactic acid synthesis during milk fermentation[J].Frontiers in Nutrition,2024,11:1441355.
[51] Ashraf A,Imran M.Causes,types,etiological agents,prevalence,diagnosis,treatment,prevention,effects on human health and future aspects of bovine mastitis[J].Animal Health Research Reviews,2020,21(1):36-49.
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