[目的]探索一种能够准确快速检测巴氏杀菌乳中碱性磷酸酶（ALP）的荧光检测方法。[方法]运用对比试验的方式,对A、B两厂家的ALP快速检测试剂盒进行试验验证。[结果]使用B厂家检测试剂盒检测巴氏杀菌乳中碱性磷酸酶,可准确快速完成检测。[结论]B厂家检测试剂盒精密度、稳定性及操作便捷性,校准符合率等方面均满足检测需求,可作为检测巴氏杀菌乳中碱性磷酸酶的检测方法。
[Objective] Explore a fluorescence detection method that can accurately and quickly detect alkaline phosphatase（ALP）in pasteurized milk. [Method] The comparative experiment was conducted to verify the ALP rapid detection kits from manufacturer A and B. [Result] Using the detection kit from manufacturer B to test for alkaline phosphatase in pasteurized milk could complete the detection accurately and quickly. [Conclusion] The detection kit from manufacturer B met the requirements in terms of precision,stability,and ease of operation,and could be used as a method for detecting alkaline phosphatase in pasteurized milk.
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