[目的] 针对鲜豆渣水分过高难以直接发酵的问题,研究干燥辅料类型及混合比例对豆渣发酵参数的影响,筛选适宜的发酵工艺。[方法] 选取木薯酒糟渣、玉米芯粉和水稻秸秆粉作为水分调节辅料,设置3 个混合比例(92∶8、84∶16和76∶24),并以纯豆渣作为高水分对照组。所有组别均接种1×107 CFU/g的植物乳杆菌,发酵30 天后测定相关指标。[结果] 玉米芯粉和水稻秸秆粉能有效降低物料水分,提高发酵品质;其中玉米芯粉组在84∶16比例下乳酸含量最高,感官品质优良。而在76∶24的高辅料比例下,玉米芯粉组出现局部霉变,提示了物料压实度与水分的临界平衡点。木薯酒糟渣组因高灰分带来的强缓冲性及缺乏水溶性碳水化合物,导致pH值显著高于其他组。[结论] 玉米芯粉按84∶16比例混合是优化豆渣发酵与营养保留的最佳工艺。
[1] Vong W C,Liu S.Biovalorisation of okara(soybean residue) for food and nutrition[J].Trends in Food Science & Technology,2016,52:139-147.
[2] Nagano T,Arai Y,Yano H,et al.Improved physicochemical and functional properties of okara,a soybean residue,by nanocellulose technologies for food development-A review[J].Food Hydrocolloids,2020,109:105964.
[3] Rahman M M,Mat K,Ishigaki G,et al.A review of okara (soybean curd residue) utilization as animal feed:Nutritive value and animal performance aspects[J].Animal Science Journal,2021,92(1):e13594.
[4] 刘烺,王红兵,陈世文,等.发酵豆渣混合饲料养猪方法探究[J].农家参谋,2024(20):53-55.
[5] Liu J,Wang K,Zhao L,et al.Investigation of supplementation with a combination of fermented bean dregs and wheat bran for improving the growth performance of the sow[J].Journal of Animal Science and Technology,2024,66(2):295-309.
[6] 普宣宣,王荣,张秀敏,等.豆渣混贮对青年期奶牛生长性能、营养物质表观消化率、瘤胃发酵及甲烷排放的影响[J].动物营养学报,2024,36(3):1724-1733.
[7] 张明,王燕,欧阳梦云.模糊数学感官评价法优化豆渣发酵工艺[J].中国酿造,2015,34(9):75-78.
[8] Broderick G A,Kang J H.Automated simultaneous determination of ammonia and total amino acids in ruminal fluid and in vitro media[J].Journal of Dairy Science,1980,63(1):64-75.
[9] Erwin E S,Marco G J,Emery E M.Volatile fatty acid analyses of blood and rumen fluid by gas chromatography[J].Journal of Dairy Science,1961,44(9):1768-1771.
[10] Madrid J,Martínez-Teruel A,Hernández F,et al.A comparative study on the determination of lactic acid in silage juice by colorimetric,high-performance liquid chromatography and enzymatic methods[J].Journal of the Science of Food and Agriculture,1999,79(12):1722-1726.
[11] 丁立人. 凯氏定氮仪测定饲料中粗蛋白含量误差因素分析[J].农业与技术,2018,38(21):17-19.
[12] Van Soest P J,Robertson J B,Lewis B A.Methods for dietary fiber,neutral detergent fiber,and nonstarch polysaccharides in relation to animal nutrition[J].Journal of Dairy Science,1991,74(10):3583-3597.
[13] Arthur Thomas T.An automated procedure for the determination of soluble carbohydrates in herbage[J].Journal of the science of food and agriculture,1977,28(7):639-642.
[14] 申希平,祁海萍,刘小宁,等.两因素非参数方差分析在SPSS中的实现[J].中国卫生统计,2013,30(6):913-914.
[15] Qiu Y,Ye S,Huang X,et al.Fermentation with a multi-strain to enhance the flavor of HongJun Tofu,a Chinese fermented okara food[J].Food Science & Technology,2023,189:115495.
[16] 杨劲松. 残次红枣发酵饲料工艺参数的优化及其对母羊育羔性能的影响[D].阿拉尔:塔里木大学,2022.
[17] 刘丽娜,王文亮,徐同成,等.乳酸菌发酵对泡菜质量的影响[J].山东农业科学,2010(1):86-89.
[18] 王向阳,杨佳航,金龙.防腐剂和酸味剂对发酵萝卜保藏作用的影响[J].中国调味品,2017,42(2):53-56.
[19] 黑梦琴,曹正宇,王志远,等.添加复合乳酸菌和糖蜜对不同水分含量苜蓿青贮品质的影响[J].草地学报,2024,32(5):1601-1609.
[20] Blajman J E,Páez R B,Vinderola C G,et al.A meta-analysis on the effectiveness of homofermentative and heterofermentative lactic acid bacteria for corn silage[J].Journal of Applied Microbiology,2018,125(6):1655-1669.
[21] Du S,You S,Jiang X,et al.Evaluating the fermentation characteristics,bacterial community,and predicted functional profiles of native grass ensiled with different additives[J].Frontiers in Microbiology,2022,13.
[22] 韩雨茜,党雪文,郭鸰,等.谷物基质对东北黏豆包质构及化学性质的影响[J].食品工业,2019,40(3):168-172.
[23] 翟静娟,曹威荣.复合微生物制剂对高粱秸秆发酵品质及其瘤胃降解特性的影响[J].饲料研究,2023,46(21):109-113.
[24] Kung L,Shaver R D,Grant R J,et al.Silage review:Interpretation of chemical,microbial,and organoleptic components of silages[J].Journal of Dairy Science,2018,101(5):4020-4033.
[25] 覃方锉,赵桂琴,焦婷,等.不同添加剂对青贮燕麦品质的影响[J].草原与草坪,2014,34(1):38-43.
[26] 赵金鹏,赵杰,李君风,等.不同添加剂对水稻秸秆青贮发酵品质和结构性碳水化合物组分的影响[J].南京农业大学学报,2019,42(1):152-159.
[27] 杨波,李龙兴,甘小波,等.不同微生物及化学物添加剂对多花黑麦草青贮发酵品质的影响[J].贵州畜牧兽医,2024,48(5):26-29.
[28] 侯金丽. 青贮玉米秸秆营养成分和发酵品质的检测技术研究[J].中国饲料,2024(12):61-64.
[29] 郭田心,阮诗诗,郭香,等.不同复合菌酶添加对中药渣青贮饲料的营养价值及发酵品质的影响[J].草业学报,2024,33(10):194-202.
[30] Zhong H,Zhou J,Abdelrahman M,et al.The effect of Lignin composition on ruminal fiber fractions degradation from different Roughage sources in Water Buffalo (Bubalus bubalis)[J].Agriculture,2021,11(10):1015.
[31] 欧阳佳良,戚如鑫,陈逸飞,等.不同青贮添加剂组合对稻秸青贮品质的影响[J].中国畜牧杂志,2019,55(9):81-85.
[32] 刘大森,唐柯楠,郑飞,等.NDF对反刍动物的作用及影响其瘤胃降解率因素分析[J].饲料工业,2018,39(10):1-5.