[目的] 本研究旨在探究丹参多糖对奶牛隐性乳房炎的治疗效果,为临床防治奶牛隐性乳房炎提供新型天然药物方案。[方法] 选取临床确诊的隐性乳房炎奶牛60 头,随机分为对照组（CG）、低剂量丹参多糖组（LG）、中剂量丹参多糖组（MG）和高剂量丹参多糖组（HG）,每组15 头,分别采用生理盐水、5、10、15 mg/kg·bw的丹参多糖进行乳房灌注治疗,连续治疗7 d。通过检测治疗前后奶牛乳汁中体细胞数（SCC）、乳成分指标,血清中炎症因子水平,分析丹参多糖的治疗效果。[结果] 丹参多糖对奶牛隐性乳房炎的治疗效果呈剂量依赖性,HG丹参多糖组治疗后第7 d乳汁SCC较对照组极显著降低（P＜0.01）,由治疗前的（286.42±35.17）×10⁴ 个/mL降至（82.15±12.34）×10⁴ 个/mL;MG、HG丹参多糖组乳汁中乳蛋白、乳糖含量显著高于CG（P＜0.05）,乳脂率无显著变化;血清中肿瘤坏死因子-α（TNF-α）、白细胞介素-6（IL-6）水平显著低于对照组（P＜0.05）,白细胞介素-10（IL-10）水平显著高于对照组（P＜0.05）。同时,HG奶牛的日均产奶量在治疗后第7 d较对照组提升12.6%,差异显著（P＜0.05）。[结论] 15 mg/kg·bw的丹参多糖乳房灌注可有效降低奶牛隐性乳房炎乳汁SCC,调节机体炎症反应,改善乳品质并提升产奶量,对奶牛隐性乳房炎具有良好的治疗效果。

[Objective] This study aimed to investigate the therapeutic effect of Salvia miltiorrhiza polysaccharide on dairy cow subclinical mastitis,so as to provide a novel natural medication regimen for its clinical prevention and treatment.[Method] A total of 60 dairy cows clinically diagnosed with subclinical mastitis were randomly allocated into the control group（CG）,low-dose Salvia miltiorrhiza polysaccharide group（LG）,medium-dose group（MG）and high-dose group（HG）,with 15 individuals per group.The cows received 7-day consecutive intramammary infusion treatments of normal-saline,5,10 and 15 mg/kg·bw Salvia miltiorrhiza polysaccharide,respectively.The therapeutic efficacy of Salvia miltiorrhiza polysaccharide was assessed by detecting milk somatic cell count（SCC）,milk composition parameters and serum inflammatory factor levels before and after treatment.[Results] The therapeutic effect of Salvia miltiorrhiza polysaccharide on dairy cow subclinical mastitis exhibited dose-dependence.On Day 7 post-treatment,the milk SCC of HG decreased extremely significantly in contrast to the control group（P＜0.01）,falling from（286.42±35.17）×10⁴ cells/mL at baseline to（82.15±12.34）×10⁴ cells/mL.Milk protein and lactose contents in MG and HG were significantly higher than those in CG（P＜0.05）,while no obvious difference was observed for milk fat percentage.Compared with the control group,serum tumour necrosis factor-α (TNF-α) and interleukin-6（IL-6）concentrations were markedly reduced,and interleukin-10（IL-10）content was significantly elevated in the polysaccharide-treated groups（P＜0.05）.Moreover,the average daily milk yield of HG dairy cows rose by 12.6 % on the 7th treatment day relative to CG,presenting statistically significant difference（P＜0.05）.[Conclusion] Intramammary infusion with 15 mg/kg·bw Salvia miltiorrhiza polysaccharide can effectively lower milk SCC,regulate systemic inflammatory responses,ameliorate milk quality and improve milk yield.Accordingly,it delivers favourable curative effects against subclinical mastitis in dairy cows.