China Dairy ›› 2023, Vol. 0 ›› Issue (2): 20-25.doi: 10.12377/1671-4393.23.02.04

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Production of Monoclonal Antibody Against Melatonin

WU Haixin1, LIAO Chenxing2, CHANG Yi2, MA Hui2, LIU Qiaoxiang2, LI Youzhi3, GUO Gang2,*, LIU Guoshi1,*   

  1. 1College of Animal Science and Technology,Beijing China Agricultural University,Beijing 100193;
    2Beijing Capital Agribusiness& Foods Group,Beijing 100028;
    3Shandong Feed and Veterinary Drug Quality Inspection Center,Jinan Shandong 250109
  • Online:2023-02-25 Published:2023-03-10

Abstract: Melatonin (MT),which is widely existed in mammals,is a common indole substance that can play a role in scavenging free radicals,regulating immunity,and regulating nerves. Here,five BALB/c mice were immunized with melatonin -OVA as the immunogen,and blood was collected for detection after five immunizations. The titer of antiserum against melatonin-OVA was determined by indirect ELISA. After ELISA screening,5 monoclonal cell lines(3G3B2,3G3C7,3G3D1,3G3F4 and 3G3G3) stably expressing melatonin antibody were obtained. The concentration of purified antibody was 0.28 mg/mL,and the titer of purified antibody reached 1∶64 000. The melatonin monoclonal antibody produced in this research laid a material foundation for further development of a rapid detection method of melatonin.

Key words: melatonin, monoclonal antibody, immunity, cell fusion, ELISA

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