China Dairy ›› 2025, Vol. 0 ›› Issue (1): 80-85.doi: 10.12377/1671-4393.25.01.13

• QUALITY CONTROL • Previous Articles     Next Articles

The Influence of Common Medium Components in Laboratory on the Detection of Lactic Acid Bacteria

SHI Beibei1, XU Jiayin1, WANG Lianfeng1, MENG Qinghong1, ZHU Minge1, WANG Dan2, LI Canguo1,*   

  1. 1 Inspection and Management Office,Mengniu Dairy Tai'an Co.,Ltd.,Tai'an Shandong 271000;
    2 Quality Inspection Department,Inner Mongolia Mengniu Dairy（Group）Co.,Ltd.,Hohhot Inner Mongolia 010000
  • Online:2025-01-25 Published:2025-02-14

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Abstract: [Objective] Lactic acid bacteria are of great significance in promoting human health. As good carriers, fermented milk and live lactic acid bacteria beverage are important sources of lactobacillus supplementation for human body. The national standards clearly specifies the quantity of lactic acid bacteria in fermented milk and live probiotic beverages, so it is important to ensure the accuracy of detection results. [Method] Common laboratory medium are selected as the interference components, and the effects of various interfering components on lactic acid bacteria culture media and national standard test results are explored through artificial addition.[Result] The total number of bifidobacterium is the most affected by rose Bengal medium and BS medium, and the least affected by VRBA medium. The total number of Streptococcus thermophilus is the most affected by TTB medium and the least affected by VRBA medium. The total number of Lactobacillus is most affected by SC medium, VRBA medium and XLD medium have the least effect on Lactobacillus. MRS AGAR medium is most affected by SC medium composition, modified MRS AGAR medium is most affected by SC medium, MC AGAR medium is mainly affected by high supplemental BS medium. [Conclusion] The results provided a basis for judging the culture status of lactic acid bacteria through the medium, colony sense and colony number changes, and provided a reference for the abnormal analysis of lactic acid bacteria detection results, and have certain guiding significance for ensuring the accuracy of lactic acid bacteria detection results.

Key words: lactic acid bacteria, total number of lactic acid bacteria, cross contamination

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