China Dairy ›› 2021, Vol. 0 ›› Issue (9): 8-20.doi: 10.12377/1671-4393.21.09.03

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Effects of Dietary Urea Supplementon Rumen Epithelial Cell proliferation, apoptosis and Epithelial Absorption and Transportation

LU Zhongyan1, ZHANG Wenxuan2, Ahela Tuoliubai2   

  1. 1Key Lab of Animal Physiology and Biochemistry, College of Veterinary Medicine,NanjingAgricultural University,Nanjing Jiangsu 210095;
    2College of Veterinary Medicine,Nanjing Agricultural University,Nanjing Jiangsu 210095
  • Online:2021-09-25 Published:2021-09-29

Abstract: This study investigated the effects of dietary urea supplement on goat rumen fermentation,epithelial cell proliferation&apoptosis,absorption and transportation of rumen epithelium. 18 Boza goats were randomly divided into 3 groups (n = 6) and fed with 3 different diets: LC group(only hay),MC group (30% concentrate)and Urea group(1% DM urea replacing soybean meal partly + 30% concentrate). The short chain fatty acids (SCFA) concentration and pH in goats rumen in Urea group and MC group were significantly higher than LC group,but there was no significant difference between these two groups. Meanwhile, Urea group had even higher rumen NH3 and plasma urea-N (BUN)concentrations than MC group. Therefore,the effects of dietary urea supplement on epithelium growth,cell cycle,mRNA expression of cell proliferation,cell apoptosis related genes and SCFA transporters which dependent on ruminal SCFA concentration and pH were similar to those in MC group. Comparing with MC group, Urea group had significant inhibitory effects on urea transport across rumen epithelium and intro-cellular pH(pHi)regulator protein mRNA expression which dependent on ruminal ammonia concentration, but those were still significantly higher than LC group.

Key words: rumen, dietary urea supplement, cell proliferation and apoptosis of rumen epithelium, absorption and transportation, urea recycling.

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