China Dairy ›› 2025, Vol. 0 ›› Issue (8): 54-60.doi: 10.12377/1671-4393.25.08.09

• DISEASE PREVENTION • Previous Articles     Next Articles

Genotyping and Drug Resistance Analysis of Toxin Genes in Clostridium perfringens from Calf Diarrhea--A Case Study of a Large-scale Dairy Farm in Shandong Province

LIU Zhimei   

  1. Da Zhuang Animal Husbandry and Veterinary Station,Yinan County,Linyi Shandong 276305
  • Online:2025-08-25 Published:2025-09-10

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Abstract: [Objective] In order to find out the etiology and pathogen resistance of diarrhea calves in a large-scale dairy farm in Shandong Province,the pathogenic identification and drug resistance analysis were carried out. [Method] A total of 18 samples of mesenteric,lung,spleen and other tissues of dead calves were collected aseptically and put into sampling bags. Through bacterial isolation and culture and biochemical test,bacterial 16 S rRNA was amplified by PCR and toxin typing was identified. The K-B agar diffusion paper method was used to test the drug resistance of the isolated strains,and 14 drug resistance genes were detected by PCR electrophoresis. [Result] The results showed that 5 strains of C.perfringens type C were detected,and the detection rate was 27.8%. The isolated bacteria showed resistance to a variety of antibiotics,and β-lactams,aminoglycosides,sulfonamides and chloramphenicol resistance genes were detected. [Conclusion] The results showed that C.perfringens type C was the pathogenic bacteria causing diarrhea in calves on dairy farms,and it was resistant to some antibiotics. The results of this study provide a reference for the prevention and control of Clostridium perfringens in dairy farms.

Key words: diarrhea calves, clostridium perfringens, toxin genotyping, drug resistance

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