China Dairy ›› 2023, Vol. 0 ›› Issue (2): 10-14.doi: 10.12377/1671-4393.23.02.02

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Determination of Melatonin Content in Cow's Milk

SHEN Zixia1, CHANG Yi2, LIU Qiaoxiang2, GUO Gang2, MA Hui2, LI Youzhi3, LIAO Chenxing2,*, LIU Guoshi1,*   

  1. 1College of Animal Science and Technology,China Agricultural University,Beijing 100193;
    2Beijing Capital Agribusiness& Foods Group,Beijing 100028;
    3Shandong Feed and Veterinary Drug Quality Inspection Center,Jinan Shandong 250109
  • Online:2023-02-25 Published:2023-03-10

Abstract: Melatonin,as a multifunctional active factor,has a very important role in livestock production. In recent years,research on high content of naturally expressed melatonin milk has been carried out on a large scale and the demand for the determination of melatonin content in milk has increased. The most common methods for melatonin determination are gas chromatography(GC),gas chromatography-mass spectrometry(GC-MS),high performance liquid chromatography(HPLC),high performance liquid chromatography-mass spectrometry(LC-MS), radioimmunoassay(RIA),enzyme-linked immunosorbent assay(ELISA),and ultraviolet spectrophotometer(UV). This paper introduced these methods in terms of principles,characteristics,advantages and disadvantages,and briefly compared the application of each method to provide some reference for the subsequent related research and application.

Key words: melatonin, concentration, milk, detection method

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