中国乳业 ›› 2022, Vol. 0 ›› Issue (5): 64-68.doi: 10.12377/1671-4393.22.05.13

• 质量安全 • 上一篇    下一篇

驴乳发酵乳源的植物乳杆菌L28鉴定及其对小鼠肠道菌群的影响

张奇1,*, 肖会姣2, 武丽辉3, 贾薇2   

  1. 1 北京城市学院生物医药学部,北京 100094;
    2 中驭（北京）医学研究院,北京 101102;
    3 农业农村部农药检定所,北京 100125
  • 出版日期:2022-05-25 发布日期:2022-06-01
  • 作者简介:张 奇（1979-）,男,辽宁大连人,博士,教授,研究方向为药学、免疫学及发酵;肖会姣（1977-）,女,湖北天门人,硕士,研究方向为营养学;武丽辉（1978-）,女,河北邢台人,硕士,高级农艺师,研究方向为农药学;贾 薇（1971-）,女,北京人,硕士,研究方向为营养学。
  • 基金资助:
    北京城市学院种子基金（KYZZ201802）; 北京市大学生创新创业项目（201911418030）

Identification of Lactobacillus plantarum L28 from Fermented Donkey Milk and its Effect on Intestinal Microflora in Mice

ZHANG Qi1,*, XIAO Huijiao2, WU Lihui3, JIA Wei2   

  1. 1 Department of Biomedicine,Beijing City University,Beijing 100094;
    2 Zhongyu（Beijing）Medical Research Institute,Beijing 101102;
    3 Institute for the Control of Agrochemicals, Ministry of Agriculture and Rural Affairs, Beijing 100125
  • Online:2022-05-25 Published:2022-06-01

PDF (PC)

32

摘要： 人类肠道内存在大量参与人体代谢并与多种疾病具备相关性的微生物。为了探索从新疆驴乳发酵乳中分离获得的菌种L28的益生功能,本研究对菌种L28进行生理生化与分子鉴定后,以其为研究对象,以抗生素致肠道功能紊乱小鼠模型为实验动物,采用平板计数法和16s rDNA测序技术,探索L28改善模型小鼠肠道的功能。结果表明,L28为植物乳杆菌,可有效地促进模型小鼠肠道中乳酸菌和肠杆菌恢复（p<0.01）,其中乳酸菌恢复更快,存在剂量依赖关系;observed species、Chao、ACE及Shannon指数提高,Simpson指数下降,进一步说明小鼠肠道菌群多样性显著提升,肠道功能得到恢复,L28菌株具有益生功能。

关键词: 植物乳杆菌L28, 肠道功能紊乱, 肠道微生态, 16s rDNA测序, 抗生素

Abstract: There are a large number of microorganisms in human gut,which participate in metabolism and are related to many diseases. In this study,L28 was identified and used as the research object,and the antibiotic induced intestinal dysfunction mouse model was used as the experimental animal. The improvement function of L28 on the mouse model intestinal was explored by the plate counting method and 16s rDNA sequencing technology. The results showed that L28 was Lactobacillus plantarum and could effectively promote the promotion of Lactobacillus and Enterobacter in the intestinal tract of model mice（p<0.01）,Lactobacillaceae recover faster, and there was a dose-dependent relationship. As the same time,observed species,Chao,ACE and Shannon indexes increased,Simpson index decreased,which indicated that the diversity of intestinal flora was significantly increased,L28 strain has probiotic function.

Key words: Lactobacillus plantarum L28, intestinal Disorders, intestinal microecology, 16S rDNA sequencing, antibiotic

中图分类号: 

  • Q93
[1] Turnbaugh P J,Ley R E,Mahowald M A,et al.An obesity-associated gut microbiome with increased capacity for energy harvest[J]. Nature,2006,444:1027-1031.
[2] Karlsson F H,Tremaroli V,Nookaew I,et al.Gut metagenome in European women with normal,impaired and diabetic glucose control[J]. Nature,2013,498:99-103.
[3] Shen Z H,Zhu C X,Quan Y S,et al.Relationship between intestinal microbiota and ulcerative colitis: Mechanisms and clinical application of probiotics and fecal microbiota transplantation[J]. World Journal of Gastroenterol,2018,24(1):5-14.
[4] Hibberd A A,Lyra A,Ouwehand A C,et al.Intestinal microbiota is altered in patients with colon cancer and modified by probiotic intervention[J]. BMJ Open Gastroenterol,2017,4:e000145.
[5] David J S.Intestinal microbiome and atherosclerosis[J]. EBioMedicine,2016,13:17-18.
[6] Chen Y C,Greenbaum J,Shen H,et al.Association between gut microbiota and bone health:potential mechanisms and prospective[J]. Journal of Clinical Endocrinology Metabolism,2017,102(10): 3635-3646.
[7] Buford T W,Willoughby D S.Impact of DHEA(S) and cortisol on immune function in aging: a brief review[J]. Applied Physiology Nutrition and Metabolism,2018,33(3):429-433.
[8] 门昌君,张国梁,王飒. 肠道微生态与人体疾病相关性研究现状[J].继续医学教育,2020,34(3):142-145.
[9] 高云,梁尚栋,穆松牛,等. 调节肠道菌群和预防肠道菌群失调动物模型探讨[J]. 中国比较医学杂志, 2005,15(3):164-166.
[1] 贾洪川, 魏华威, 姚华. 戴尔沃牛奶抗生素检测试剂盒灵敏度验证及操作注意事项[J]. 中国乳业, 2021, 0(5): 78-80.
[2] 董菡, 张倩瑶, 成玉梁, 于航, 谢云飞, 姚卫蓉, 郭亚辉, 钱和. 乳制品抗生素快检试纸条的研究进展[J]. 中国乳业, 2020, 0(8): 67-72.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
ISSN 1671-4393
CN 11-4768/S
主管：中华人民共和国农业农村部
主办：中国农业科学院农业信息研究所
地址：北京市海淀区中关村南大街12号
邮编：100081
电话：010-82106772
E-mail：zhgry@caas.cn

版权所有 © 《中国乳业》编辑部