脉冲场凝胶电泳对不同牛舍中大肠杆菌的分析鉴定

中国乳业 ›› 2020, Vol. 0 ›› Issue (5): 51-53.

脉冲场凝胶电泳对不同牛舍中大肠杆菌的分析鉴定

李庆雷, 王金新^*, 刘鹏, 付嵩, 马驰

山东高速生物工程有限公司

出版日期:2020-05-25 发布日期:2021-02-24
通讯作者: *王金新（1991-）,男,山东枣庄人,硕士,研究方向为反刍动物营养。
作者简介:李庆雷（1984-）,男,山东德州人,硕士,研究方向为环境微生物学。

Online:2020-05-25 Published:2021-02-24

摘要/Abstract

摘要： 为了研究奶牛场不同牛舍牛群间大肠杆菌的传播情况,采用Andersen-6级空气微生物样品收集器,分别在现代化奶牛场的泌乳牛舍、青年牛舍和犊牛舍收集空气样品,并采集泌乳牛的粪便,分离、鉴定大肠杆菌相似性,运用脉冲场凝胶电泳（PFGE）技术,进行同源性鉴定分析后,可知从泌乳牛的粪便中分离到的大肠杆菌与从牛舍内空气中分离到的部分大肠杆菌（23%）相似性可达85%以上。结果表明,从泌乳牛的粪便中分离的大肠杆菌能够对环境造成生物污染,在泌乳牛舍中形成环境微生物气溶胶,并在不同牛舍间随空气传播,该结果对奶牛场牛舍环境消毒具有理论指导作用,并具有公共卫生学意义。

关键词: 奶牛场, 牛舍, 生物安全, 大肠杆菌, 气溶胶, 脉冲场凝胶电泳（PFGE）

李庆雷, 王金新, 刘鹏, 付嵩, 马驰. 脉冲场凝胶电泳对不同牛舍中大肠杆菌的分析鉴定[J]. 中国乳业, 2020, 0(5): 51-53.

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