四种血酮检测仪对奶牛酮病诊断效果的比较分析

doi:10.12377/1671-4393.22.05.11

中国乳业 ›› 2022, Vol. 0 ›› Issue (5): 54-59.doi: 10.12377/1671-4393.22.05.11

四种血酮检测仪对奶牛酮病诊断效果的比较分析

邓鑫¹, 周雨舟¹, 林敏杰¹, 诸葛树妹¹, 何家康^2,*

1 桂林优利特医疗电子有限公司,广西桂林 541000;
2 广西大学动物科学技术学院,广西南宁 530005

出版日期:2022-05-25 发布日期:2022-06-01
通讯作者: ^*何家康（1976-）,男,广西壮族自治区崇左人,博士,教授,研究方向为动物疫病快速诊断技术。
作者简介:邓鑫（1989-）,男,广西壮族自治区桂林人,硕士,助理兽医师,研究方向为动物疫病快速诊断技术;周雨舟（1995-）,女,壮族,广西壮族自治区来宾人,助理工程师,研究方向为动物疫病快速诊断技术（并列第一作者）;林敏杰（1984-）,男,广西壮族自治区桂林人,本科,中级工程师,研究方向为动物疫病快速诊断技术;诸葛树妹（1993-）,女,广西壮族自治区桂林人,本科,助理工程师,研究方向为动物疫病快速诊断技术。
基金资助:
广西创新驱动发展专项资金项目（桂科AA17204057-4）

Comparative Analysis of the Diagnostic Effect of Four Blood Ketone Meters on Dairy Cow Ketosis

DENG Xin¹, ZHOU Yuzhou¹, LIN Minjie¹, ZHUGE Shumei¹, HE Jiakang²

1 URIT Medical Electronics Co.Ltd.,Guilin Guangxi 541000;
2 College of Animal Science and Technology,Guangxi University,Nanning Guangxi 530005

Online:2022-05-25 Published:2022-06-01

摘要/Abstract

摘要： 目的比较四种血酮检测仪和生化分析仪的测试奶牛血液中β-羟丁酸的浓度结果,分析血酮检测仪对奶牛酮病临床诊断的效果,为奶牛场对血酮检测仪的使用选择提供依据。方法本试验选取41 头2~5 胎次、产后5~25 天的泌乳奶牛经尾静脉采血进行β-羟丁酸浓度的测试。应用四种血酮检测仪（桂林优利特、美国雅培、北京怡成和台湾暐世）与生化分析仪对比检测奶牛血液中β-羟丁酸含量的相关性和准确度。结果四种血酮检测仪与生化分析仪相关性高,线性回归分析结果均>0.95;当测试结果绝对偏差≤±0.2或相对偏差≤±20%时,四种血酮检测仪的合格率均达100%;四种血酮仪测试的均值与生化分析测试结果进行配对T检验,结果显示差异均无统计学意义（P>0.05）。结论四种血酮检测仪的临床测试性能与生化分析仪接近,均满足用于奶牛血酮检测的要求,可用于奶牛酮病的筛查,其中桂林优利特品牌的血酮仪性价比相对较高,更适合奶牛场用于奶牛酮病的快速筛查。

关键词: 奶牛, 酮病, 血酮检测仪, β-羟丁酸

Abstract: Objective：To compare the results of testing the content of β-hydroxybutyric acid in the blood of dairy cows with four blood ketone meters and biochemical meters,this paper analyzed the effect of blood ketone meters on the clinical diagnosis of ketosis in dairy cows,and provided a basis for the selection of blood ketone meters for dairy farms. Methods：Fourty-one dairy cows with 2~5 parities and 5~25 days postpartum were collected through the tail vein for blood. Four blood ketone meters （Guilin Urit,American Abbott,Beijing Yicheng and Taiwan Visteon）were used to compare with biochemical meters to detect β-hydroxybutyric acid in dairy cows blood. Results：The four blood ketone detectors have high correlation with the biochemical analyzer,and the linear regression analysis results >0.95;when the absolute deviation of the test result was≤±0.2 or the relative deviation was≤±20%,the pass rate of the four blood ketone detectors all reach 100%.The P values of the four blood ketone meter tests and the biochemical analysis test results were subjected to a paired T test,and the results showed that there was no statistical significant（P>0.05）.Conclusion：The clinical test performance of the four blood ketone detectors was close to that of the biochemical analyzer,and they all met the requirements for the detection of blood ketones in dairy cows,and can be used for the screening of ketosis in dairy cows. Among them,the blood ketone meter of Guilin Urit was relatively cost-effective. It was more suitable for dairy farms to quickly screen for ketosis in dairy cows.

Key words: dairy cows, ketosis, blood ketone meters, β-hydroxybutyric acid

中图分类号:

S858.23

邓鑫, 周雨舟, 林敏杰, 诸葛树妹, 何家康. 四种血酮检测仪对奶牛酮病诊断效果的比较分析[J]. 中国乳业, 2022, 0(5): 54-59.

DENG Xin, ZHOU Yuzhou, LIN Minjie, ZHUGE Shumei, HE Jiakang. Comparative Analysis of the Diagnostic Effect of Four Blood Ketone Meters on Dairy Cow Ketosis[J]. China Dairy, 2022, 0(5): 54-59.

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四种血酮检测仪对奶牛酮病诊断效果的比较分析

Comparative Analysis of the Diagnostic Effect of Four Blood Ketone Meters on Dairy Cow Ketosis

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