实验室常见培养基成分对乳酸菌检测的影响

doi:10.12377/1671-4393.25.01.13

中国乳业 ›› 2025, Vol. 0 ›› Issue (1): 80-85.doi: 10.12377/1671-4393.25.01.13

实验室常见培养基成分对乳酸菌检测的影响

师倍倍¹, 徐佳荫¹, 王连凤¹, 孟庆红¹, 朱明娥¹, 王丹², 李灿国^1,*

1 蒙牛乳业泰安有限责任公司检验管理处,山东泰安 271000;
2 内蒙古蒙牛乳业（集团）股份有限公司质量管理部,内蒙古呼和浩特 010000

出版日期:2025-01-25 发布日期:2025-02-14
通讯作者: ^*李灿国（1995-）,男,山东泰安人,硕士,研究方向为乳品质量安全。
作者简介:师倍倍（1988-）,女,山东枣庄人,本科,中级工程师,研究方向为乳品微生物检测;徐佳荫（1981-）,女,山东泰安人,本科,中级工程师,研究方向为乳品质量控制;王连凤（1984-）,女,黑龙江哈尔滨人,本科,中级工程师,研究方向为乳品质量控制;孟庆红（1975-）,女,内蒙古通辽人,蒙古族,硕士,高级工程师,研究方向为食品质量安全;朱明娥（1982-）,女,山东泰安人,本科,中级工程师,研究方向为乳品微生物检测;王丹（1983-）,女,内蒙古呼和浩特人,硕士,高级工程师,研究方向为质量检验管理。
基金资助:
山东省重点研发计划项目（2023CXGC010711）

The Influence of Common Medium Components in Laboratory on the Detection of Lactic Acid Bacteria

SHI Beibei¹, XU Jiayin¹, WANG Lianfeng¹, MENG Qinghong¹, ZHU Minge¹, WANG Dan², LI Canguo^1,*

1 Inspection and Management Office,Mengniu Dairy Tai'an Co.,Ltd.,Tai'an Shandong 271000;
2 Quality Inspection Department,Inner Mongolia Mengniu Dairy（Group）Co.,Ltd.,Hohhot Inner Mongolia 010000

Online:2025-01-25 Published:2025-02-14

摘要/Abstract

摘要： [目的] 乳酸菌对促进人体健康具有重要意义。作为良好载体,发酵乳和活菌型乳酸菌饮料是人体补充乳酸菌的重要来源。我国国家标准明确规定发酵乳、活菌型乳酸菌饮料中乳酸菌数量,对保证检测结果准确有重要意义。[方法] 选择常见实验室培养基作为干扰成分,通过人为添加,探究各干扰成分对乳酸菌培养基、国标法检测结果的影响。[结果] 双歧杆菌总数受孟加拉红培养基、BS培养基影响最大,VRBA培养基影响最小。嗜热链球菌总数受TTB培养基影响最大,VRBA培养基影响最弱。乳杆菌总数受SC培养基影响最大,受VRBA培养基、XLD培养基影响最小。MRS琼脂培养基感官受SC培养基成分影响最大,改良MRS琼脂培养基受SC培养基影响最大,MC琼脂培养基主要受高添加量BS培养基影响。[结论] 本试验结果为通过培养基、菌落感官及菌落数量变化判断乳酸菌培养状态提供依据,为乳酸菌检测结果异常分析提供参考,对保证乳酸菌检测结果准确有一定的指导意义。

关键词: 乳酸菌, 乳酸菌总数, 交叉污染

Abstract: [Objective] Lactic acid bacteria are of great significance in promoting human health. As good carriers, fermented milk and live lactic acid bacteria beverage are important sources of lactobacillus supplementation for human body. The national standards clearly specifies the quantity of lactic acid bacteria in fermented milk and live probiotic beverages, so it is important to ensure the accuracy of detection results. [Method] Common laboratory medium are selected as the interference components, and the effects of various interfering components on lactic acid bacteria culture media and national standard test results are explored through artificial addition.[Result] The total number of bifidobacterium is the most affected by rose Bengal medium and BS medium, and the least affected by VRBA medium. The total number of Streptococcus thermophilus is the most affected by TTB medium and the least affected by VRBA medium. The total number of Lactobacillus is most affected by SC medium, VRBA medium and XLD medium have the least effect on Lactobacillus. MRS AGAR medium is most affected by SC medium composition, modified MRS AGAR medium is most affected by SC medium, MC AGAR medium is mainly affected by high supplemental BS medium. [Conclusion] The results provided a basis for judging the culture status of lactic acid bacteria through the medium, colony sense and colony number changes, and provided a reference for the abnormal analysis of lactic acid bacteria detection results, and have certain guiding significance for ensuring the accuracy of lactic acid bacteria detection results.

Key words: lactic acid bacteria, total number of lactic acid bacteria, cross contamination

师倍倍, 徐佳荫, 王连凤, 孟庆红, 朱明娥, 王丹, 李灿国. 实验室常见培养基成分对乳酸菌检测的影响[J]. 中国乳业, 2025, 0(1): 80-85.

SHI Beibei, XU Jiayin, WANG Lianfeng, MENG Qinghong, ZHU Minge, WANG Dan, LI Canguo. The Influence of Common Medium Components in Laboratory on the Detection of Lactic Acid Bacteria[J]. China Dairy, 2025, 0(1): 80-85.

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实验室常见培养基成分对乳酸菌检测的影响

The Influence of Common Medium Components in Laboratory on the Detection of Lactic Acid Bacteria

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