奶牛粪源大肠埃希氏菌血清分型、系统进化分群及毒力基因分布

doi:10.12377/1671-4393.25.06.14

摘要/Abstract

摘要： [目的]为研究奶牛腹泻粪源大肠埃希氏菌血清分型、系统分群及毒力基因分布。[方法]试验采集自山东部分奶牛场有腹泻症状的奶牛新鲜粪便147 份,采用细菌常规分离培养、生化试验和细菌16S rDNA的PCR扩增分子生物学技术,证实分离菌株为致病性大肠埃希氏菌,并进一步对分离菌株进行血清型、系统进化分群和毒力基因检测,分析毒力基因在系统进化分群中的分布情况。[结果]鉴定出62 株大肠埃希氏菌,9 种致病性血清分型,其中,肠侵袭性（EIEC）、肠致病性（EPEC）、肠产毒性（ETEC）大肠埃希氏菌血清型,分别占17.74%、24.19%、19.35%,优势血清型为O125∶K70（B15）和O124∶K72。18 株属于A群,12 株属于B1群,15 株属于B2群,9 株属于D群,8 株未确定系统进化分群。毒力基因ibeB、mat和fimC的分布率较高,分别为100.00%、95.16%、91.94%,所有菌株至少分布4 种毒力基因、9 种毒力基因组合形式。毒力基因与系统进化分群关联分析显示,在4 种分群中ibeB分布率均为100%,在B1群中均能检测出17 种毒力基因。[结论]山东省部分地区奶牛场奶牛粪源大肠埃希氏菌血清分型和系统进化分群具有多样性,携带多种毒力基因,应引起重视,加强监测和防控。

关键词: 奶牛粪源, 大肠埃希氏菌, 血清分型, 系统进化分群, 毒力基因

Abstract: In order to study the serotyping,phylogenetic clustering and virulence gene distribution of Escherichia coli isolated from dairy cow manure with diarrhea in dairy cows,147 fresh feces of dairy cows with diarrhea symptoms were collected from some dairy farms in Shandong Province.The isolated strain was confirmed to be pathogenic Escherichia coli by routine bacterial isolation and culture,biochemical test and PCR amplification of bacterial 16S rDNA molecular biology technology.The serotype,phylogenetic clustering and virulence gene of the isolated strain were further detected,and the distribution of virulence gene in the phylogenetic clustering was analyzed.The results showed that 62 strains of Escherichia coli were identified and 9 pathogenic serotypes were identified.Among them,intestinal invasion（EIEC）,intestinal pathogenicity（EPEC）,and enterotoxigenic（ETEC） E.coli serotypes accounted for 17.74%,24.19%,and 19.35%,respectively.The dominant serotyping were O125∶K70（B15） and O124∶K72.18 strains belonged to group A,12 strains belonged to group B1,15 strains belonged to group B2,9 strains belonged to group D,and 8 strains could not determine the phylogenetic clustering.The distribution rates of virulence genes ibeB,mat and fimC were high,which were 100.00%,95.16% and 91.94%,respectively.At least 4 virulence gene and 9 virulence gene combinations were distributed in all strains.The correlation analysis between virulence gene and phylogenetic clustering showed that the distribution rate of ibeB in the four groups was 100%,and 17 virulence gene were detected in group B1.The results showed that the serotyping and phylogenetic clustering of pathogenic Escherichia coli in dairy cow with diarrhea in some areas of Shandong Province were diverse,carrying a variety of virulence gene,which should be paid attention to and strengthened monitoring and prevention and control.

Key words: dairy cow manure, Escherichia coli, serotyping, phylogenetic clustering, virulence gene

亓爱杰. 奶牛粪源大肠埃希氏菌血清分型、系统进化分群及毒力基因分布[J]. 中国乳业, 2025, 0(6): 80-86.

QI Aijie. Serotyping,Phylogenetic Clustering and Virulence Gene Distribution of Escherichia coli from Dairy Cow Manure[J]. China Dairy, 2025, 0(6): 80-86.

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