奶牛子宫内膜炎乳房链球菌耐药性与毒力基因检测分析

doi:10.12377/1671-4393.23.12.09

摘要/Abstract

摘要： [目的]了解奶牛子宫内膜炎乳房链球菌的耐药性和携带毒力基因。[方法]采集香河县5 家奶牛场患子宫内膜炎奶牛的子宫黏液样本76 份,用PCR鉴定分离菌株的乳房链球菌,采用微量肉汤稀释法测定8 种抗菌药对乳房链球菌的最小抑菌浓度（MIC）,判断其耐药性。用PCR检测乳房链球菌携带的10 种毒力基因。[结果]PCR检测出18 株乳房链球菌,总分离率23.68%。18株乳房链球菌对克林霉素、庆大霉素、四环素耐药性较高,耐药率分别为66.67%、88.89%、72.22%。所有菌株均携带slp、sua、gapC、fpb、cfu和pauA,而hasA、hasB、acdA检出率分别为38.89%、50.00%、33.33%,未检出hasC。[结论]该地区乳房链球菌是奶牛子宫内膜炎主要致病菌之一,耐药性较严重,毒力基因流行广泛,应加强监测,合理用药。

关键词: 奶牛, 子宫内膜炎, 乳房链球菌, 耐药, 毒力基因

Abstract: [Objective]To understand the drug resistance and virulence genes of Streptococcus uberis in dairy cows with endometritis.[Method]76 uterine mucus samples of streptococcus uberis in dairy cows with endometritis were collected from 5 dairy farms in Xianghe County.Streptococcus uberis was identified by PCR method.The minimum inhibitory concentration（MIC） of 8 antibiotics against Streptococcus uberis was determined by micro-broth dilution method to determine its drug resistance.PCR method was used to detect Streptococcus uberis carrying 10 virulence genes.[Result]18 strains of Streptococcus uberis were detected by PCR method,and the total isolation rate was 23.68%.18 strains of Streptococcus lactis were highly resistant to clindamycin, gentamicin and tetracycline, and the resistance rates were 66.67%, 88.89% and 72.22% respectively,respectively.All strains carried slp,sua,gapC,fpb,cfu and pauA,while the detection rates of hasA,hasB and acdA were 38.89%,50.00% and 33.33%,respectively,and hasC was not detected.[Conclusion]Streptococcus uberis was one of the main pathogenic bacteria in dairy cow with endometritis in this area.The drug resistance was serious,and the virulence genes were widely prevalent.The monitoring should be strengthened and the drug should be used rationally.

Key words: dairy cow, endometritis, Streptococcus uberis, drug resistance, virulence gene

刘志强. 奶牛子宫内膜炎乳房链球菌耐药性与毒力基因检测分析[J]. 中国乳业, 2023, 0(12): 41-46.

LIU Zhiqiang. Analysis of Drug Resistance and Virulence Gene Detection of Streptococcus uberis in Dairy Cow with Endometritis[J]. China Dairy, 2023, 0(12): 41-46.

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