中国乳业 ›› 2023, Vol. 0 ›› Issue (9): 74-79.doi: 10.12377/1671-4393.23.09.12

• 奶牛繁殖与管理专题 • 上一篇    下一篇

奶牛胚胎移植技术要点及应用前景

操礼军1, 汪晖2, 杜婷婷1, 刘莉1,*, 胡永青1,*   

  1. 1 新疆畜牧科学院畜牧业质量标准研究所,新疆乌鲁木齐 830000;
    2 新疆农业科学院农业质量标准与检测技术研究所,新疆乌鲁木齐 830000
  • 出版日期:2023-09-25 发布日期:2023-10-16
  • 通讯作者: * 刘 莉(1988-),女,新疆塔城人,硕士,高级畜牧师,研究方向为奶业研究与奶畜遗传育种;胡永青(1970-),男,湖南双峰人,专科,高级畜牧师,研究方向为奶业信息化、奶业经济等。
  • 作者简介:操礼军(1973-),男,安徽桐城人,本科,高级畜牧师,研究方向为遗传育种;汪 晖(1986-),女,新疆乌鲁木齐人,本科,实验师,研究方向为农产品品质检测与评价;杜婷婷(1997-),女,新疆奎屯人,硕士,研究方向为食品营养与安全。
  • 基金资助:
    自治区重大科技专项-新疆奶业创新项目(2020A01001-4-3-1)

Key Points and Application Prospects of Cow Embryo Transfer Technology

CAO Lijun1, WANG Hui2, DU Tingting1, LIU Li1,*, HU Yongqing1,*   

  1. 1Institute of Animal Husbandry Quality Standards,Xinjiang Academy of Animal Husbandry Sciences,Urumqi Xinjiang 830000;
    2Institute of Agricultural Quality Standards and Testing Technology,Xinjiang Academy of Agricultural Sciences,Urumqi Xinjiang 830000
  • Online:2023-09-25 Published:2023-10-16

摘要: 胚胎移植方案增加了遗传改良并缩短了世代间隔。因此,胚胎移植作为一种有利的技术,有助于在短时间内实现遗传进步和增加有价值的育种者数量。牛胚胎移植技术涉及供体和受体动物的选择和管理,以及胚胎的收集和移植。在牛中使用胚胎移植的主要目标是提高有价值的雌性的繁殖率。因此,有价值的雌性的后代数量增加到至少比正常情况下高出5 倍的水平。体内胚胎生产的阶段包括供体动物和受体动物的选择,所选供体和受体的发情周期的同步,诱导超排卵和随后的供体授精,收集(通过子宫冲洗),胚胎的评估和移植。牛胚胎移植计划的成功和经济取决于多种因素:在任何胚胎移植计划中,使用高质量的精液和高比例的正常运动细胞是非常关键的一步;转移的对象必须是与供体处于同一周期的受者;受赠者必须具有经证实的生殖能力,无先天性或传染性疾病,以获得高受孕率,并具有健壮的体型,以避免难产问题。目前胚胎移植已广泛应用在遗传改良、种畜繁育和保种、疾病控制、基因工程研究等方面。随着技术的发展,胚胎移植必将在实际生产中发挥更多的应用价值。

关键词: 奶牛, 胚胎移植, 应用, 前景

Abstract: The embryo transfer program has increased genetic improvement and shortened generation spacing. Therefore, embryo transfer,as a favorable technology,can help achieve genetic progress and increase the number of valuable breeders in a short period of time. The technology of bovine embryo transfer involves the selection and management of donor and recipient animals,as well as the collection and transfer of embryos. The main goal of using embryo transfer in cattle is to improve the reproductive rate of valuable females. Therefore,the number of offspring of valuable females increases to at least five times higher than normal. The stages of in vivo embryo production include the selection of donor and recipient animals, synchronization of the estrous cycle of the selected donor and recipient,induction of superovulation and subsequent donor insemination,collection (through uterine flushing), evaluation and transfer of embryos. The success and economy of the bovine embryo transfer program depend on several factors. The use of high-quality semen and a high proportion of normal motor cells is a crucial step in any embryo transfer plan. The transferred object must be a recipient in the same cycle as the donor. The recipient must have proven reproductive ability,no congenital or infectious diseases,in order to achieve a high fertility rate,and have a robust body shape to avoid difficult childbirth problems. At present,embryo transfer has been widely used in genetic improvement,breeding and conservation of livestock,disease control,genetic engineering research,and other fields. With the development of technology,embryo transfer will undoubtedly play a more valuable role in practical production.

Key words: cows, embryo transfer, application, prospect

[1] Pasqualino L,Grazyna P,Maria D,et al.Embryo transfer and related technologies in sheep reproduction[J].Reproduction Nutrition Development,1998,38(6):615-628.
[2] Heape W. Preliminary note on the transplantation and growth of mammalian ova within a uterine foster mother[J]. Proceedings of the Royal Society,1891,48,457-459.
[3] Warwick B L,Barry R O,Horlacher W R.Results of mating rams to Angora female goats[J]. Journal of Animal Science,1935(1):225-227.
[4] Wilmut I,Rowson L E .Experiments on the low-temperature preservation of cow embryos[J].The Veterinary Record,1973,92(26):686-690.
[5] Philips P.& Jahinke, M. Embryo transfer (Technique,Donors, and Recipients)[J].Articles in Veterinary Clinics of North America Food animal practice,2016:366-384.
[6] Mebratu B,Fesseha H,Goa E.Embryo transfer in cattle production and its principle and applications[J].International Journal of Phar & Biomedi Research,2020,7(1):40-54
[7] Dessalegn G.A review of embryo transfer technology in cattle[J].Global Journal of Animal Scientific Research,2015,3(2):562-575.
[8] Hasler J.The current status and future of commercial embryo transfer in cattle[J].Animal Reproduction Science,2003,79:245-264.
[9] Voelkel S A,Hu Y X.Direct transfer of frozen-thawed bovine embryos[J].Theriogenology,1992,37(1):23-37.
[10] The control of follicular wave development for self-appointed embryo transfer programs in cattle[J].Theriogenology,2002,57(1):53-72.
[11] 薛洁,刘文浩,吕湾,等. 奶牛胚胎移植技术研究进展[J].中国奶牛,2018(9):22-26
[12] Hasler,John F.Forty years of embryo transfer in cattle: A review focusing on the journal Theriogenology,the growth of the industry in North America,and personal reminisces[J].Theriogenology,2014,81(1):152-169.
[13] Bó G A,Mapletoft R J .Evaluation and classification of bovine embryos[J].Animal Reproduction,2013,10(3):344-348.
[14] 张书汁,周敏,牛辉. 奶牛胚胎移植关键技术及应用前景[J].农业科学,2017,3(下):51-52
[15] George E,Seidel J,Sarah M.Training manual for embryo transfer in cattle[M].(FAO)Animal Production and Health,2005.
[16] 王志成,苏昶祺,陈仕毅,等.体外受精和胚胎移植技术在肉牛双胎生产中的应用研究进展[J].中国畜牧杂志,2022,58(7):8-14.
[17] Bondioli K R,Ellis S B,Pryor J H,et al.The use of male-specific chromosomal DNA fragments to determine sex of bovine preimplantation embryos[J].Theriogenology,1989,31:95-104.
[18] Alison L Van Eenennaam. Application of genome editing in farm animals[J]. Transgenic Research,2019,8(28):93-100
[19] Nagai S W T.Survival of embryos and calves derived from somatic cell nuclear transfer in cattle:A nationwide survey in Japan[J].Animal Science Journal,2011, 82(2):360-365.
[1] 胡智辉, 谷栗琨, 魏玉东, 韩燕, 王欢, 朱妮, 郝海生, 赵善江, 朱化彬. 常见传染性疾病对奶牛繁殖性能的影响及其研究进展[J]. 中国乳业, 2023, 0(9): 3-11.
[2] 王欢, 赵慧秋, 谷栗琨, 赵善江, 胡智辉, 朱妮, 郝海生, 朱化彬. 乳房炎对奶牛繁殖性能的影响[J]. 中国乳业, 2023, 0(9): 12-17.
[3] 王建中, 张恒, 李晓光, 王刚, 张廷青. 奶牛犬新孢子虫流产诊疗防[J]. 中国乳业, 2023, 0(9): 18-27.
[4] 陈勇, 杨青通, 曹高寨, 李强, 姚珍珍, 邵亚群. 两种孕酮缓释栓对比研究[J]. 中国乳业, 2023, 0(9): 34-40.
[5] 付畅. 奶牛产后常见繁殖疾病防控[J]. 中国乳业, 2023, 0(9): 41-46.
[6] 郭宁宁, 范鑫, 周娟, 季晨, 周博文, 姜宏尧, 韩俊平. B超声像技术在奶牛场繁殖工作中的应用[J]. 中国乳业, 2023, 0(9): 47-53.
[7] 董飞, 徐伟, 姜兴刚, 马志愤. 成母牛配种前发病对配种受胎率的影响分析[J]. 中国乳业, 2023, 0(9): 54-59.
[8] 李佳祺. 奶牛产后生殖系统疾病的病因及防控措施[J]. 中国乳业, 2023, 0(9): 65-69.
[9] 刘彩娟, 王永信, 任亮. 东北奶牛场魏氏梭菌病病因分析及防控措施[J]. 中国乳业, 2023, 0(9): 84-88.
[10] 王礞礞, 王建磊, 苟文强, 田园, 王晶. 国外奶牛乳房炎研究进展——基于Web of Science 核心数据库[J]. 中国乳业, 2023, 0(9): 89-97.
[11] 景战蕾. 奶牛蹄疣的综合防控[J]. 中国乳业, 2023, 0(9): 98-101.
[12] 韩萌, 张子淇, 王礞礞, 田园, 王晶. 选择性干奶期治疗在奶牛场中的应用与关键点控制[J]. 中国乳业, 2023, 0(9): 102-107.
[13] 刘琴, 胡秀山, 夏海晶, 张泉鹏, 周亚平, 周金平. 产后奶牛灌服丙二醇对血浆葡萄糖和酮体含量的影响[J]. 中国乳业, 2023, 0(8): 33-38.
[14] 胡肄农, 柏宗春, 朱红宾, 田雨. 奶牛智慧养殖生态环境、生理健康与生长性能数字化表征指标体系研究[J]. 中国乳业, 2023, 0(8): 46-49.
[15] 许涛. 奶牛亚急性瘤胃酸中毒的发病机理及其营养调控措施[J]. 中国乳业, 2023, 0(8): 57-63.
Viewed
Full text


Abstract

Cited

  Shared   
  Discussed   
No Suggested Reading articles found!