1,3-二油酸-2-棕榈酸甘油三酯,又名结构油脂OPO,是一种特殊的脂肪酸分子结构,其可作为母乳脂肪替代物之一。目前,企业在检测1,3-二油酸-2-棕榈酸甘油三酯方面多采用的是企业非标方法,而在采用非标方法之前,一般需对该检测方法进行方法确认,而组分测量不确定度评估是方法确认过程中最为重要的一个环节。本文采用气相色谱法,从浓度、称量、定容及仪器四个方面所引入不确定度对该检测方法不确定度进行评估。方法不确定度为U=5.04%（k=2）,符合U≤10%一般性要求。

1,3-dioleic-2-palmitic triglyceride,also known as OPO,is a special fatty acid molecular structure,which can be used as one of the fat substitutes of breast milk. At present,enterprises mostly use the enterprise non-standard method in the detection of 1,3-dioleic acid-2-palmitic acid triglyceride. Before using the non-standard method, it is generally necessary to confirm the detection method, and the evaluation of component measurement uncertainty is the most important link in the process of method confirmation. In this paper,gas chromatography was used to evaluate the uncertainty of this detection method from four aspects: concentration,weighing,constant volume and instrument itself. The uncertainty of the method was U=5.04%（k=2）, which met the general requirement of U≤10%.