[目的]评估乳粉中金黄色葡萄球菌检测结果的不确定度。[方法]依照食品安全国家标准《GB 4789.10—2016 食品微生物学检测 金黄色葡萄球菌检验》中的第二法和《JJF 1059.1—2012测量不确定度评定与表示》,以标准质控品的奶粉为例,分析样品制备、稀释过程、加样过程及重复性检测引入的不确定度。[结果]乳粉中金黄色葡萄球菌检测结果的扩增不确定度U=0.108。[结论]本研究建立的方法适合类检验条件下金黄色葡萄球菌不确定的评定。
[Objective]To evaluate the uncertainty of the detection results of Staphylococcus aureus in milk powder. [Method]According to the second method of GB 4789.10-2016 National food safety standard-Food microbiological Detection of Staphylococcus aureus and JJF 1059.1-2012 Evaluation and Representation of Uncertainty Measurement,taking the milk powder of the standard quality control product as an example,analyzed the uncertainty introduced by sample preparation,dilution process,sample addition process,and repeatability testing. [Result]The amplification uncertainty of the detection results of Staphylococcus aureus in milk powder was U=0.108. [Conclusion]The method established in this study is suitable for the uncertainty assessment of Staphylococcus aureus under similar test conditions.
[1] 林碧莲, 柯振华. 2017~2019年福建省预包装食品中致病菌污染状况调查与分析[J]. 食品安全质量检测学报,2020, 11(13):4207-4213.
[2] Wang X,Meng J,Zhang J,et al.Characterization of Staphylococcus aureus isolated from powdered infant formula milk and infant rice cereal in China[J]. International Journal of Food Microbiology,2012,153(1-2):142-147.
[3] Szczuka E,Porada K,Wesolowska M,et al.Occurrence and characteristics of Staphylococcus aureus isolated from dairy Products[J]. Molecules,2022,27(14):4649.
[4] Alghizzi M,Shami A.The prevalence of Staphylococcus aureus and methicillin resistant Staphylococcus aureus in milk and dairy products in Riyadh,Saudi Arabia[J]. Saudi Journal of Biological Sciences,2021,28(12):7098-7104.
[5] 姜华,焦阳,李远宏,等. 多重PCR检测婴幼儿配方奶粉中3种食源性致病菌[J]. 食品工业科技,2018,39(14):213-218.
[6] 徐文文,宋惠月,梁玉林,等. 环介导等温扩增技术检测不同乳制品常见食源性致病菌[J]. 食品安全质量检测学报,2021,12(2):546-551.
[7] Liu S,Wang B,Sui Z, et al.Faster detection of Staphylococcus aureus in milk and milk powder by flow cytometry[J]. Foodborne Pathogens and Disease,2021,18(5):346-353.
[8] Sun R,Zou H,Zhang Y,et al.Vancomycin recognition and induced-aggregation of the Au nanoparticles through freeze-thaw for foodborne pathogen Staphylococcus aureus detection[J]. Analytica Chimica Acta,2022,1190:339253.
[9] 秦婧,王琳帆,魏思宇,等. 国际FAPAS乳粉中金黄色葡萄球菌定量检测能力验证结果与分析[J]. 食品安全导刊,2021(26):82-83.
[10] 张华,姜庆,吴迪,等. 奶粉中金黄色葡萄球菌定量检测盲样考核结果与分析[J]. 现代食品,2022,28(7):119-121.
[11] 朱文斌,周浩,朱虹霖,等. 探究稀释液温度对金黄色葡萄球菌定量检测的影响[J]. 食品安全质量检测学报,2019,10(9):2782-2785.
[12] 雷兰兰,康婕,王瑛,等. 食品中金黄色葡萄球菌定量检验能力验证的质量控制[J]. 食品安全导刊,2022(4):61-63.
[13] 范鹏飞,顾春华,杨陶丽薇,等. 显色平板与Baird-Parker平板在金黄色葡萄球菌能力验证中的比较分析[J]. 甘肃科技纵横,2021,50(11):28-31.
[14] 补充发布单位中华人民共和国国家卫生和计划生育委员会国家食品药品监督管理总局.GB4789.10—2016 食品安全国家标准食品微生物学检验金黄色葡萄球菌检验[S]
[15] 补充发布单位国家质量监督检验检疫总局.JJF1059.1-2012 测量不确定度评定与表示[S]
[16] 徐丽萍,商晓春,舒青青. 质控金黄色葡萄球菌计数结果不确定度评定[J]. 中国卫生检验杂志,2011,21(3):654-656.
