九龙藏黄牛和九龙牦牛β-酪蛋白基因型分析

doi:10.12377/1671-4393.21.09.22

中国乳业 ›› 2021, Vol. 0 ›› Issue (9): 120-124.doi: 10.12377/1671-4393.21.09.22

九龙藏黄牛和九龙牦牛β-酪蛋白基因型分析

赵梦波, 金素钰, 黄林, 郑玉才^*

西南民族大学畜牧兽医学院,四川成都 610041

出版日期:2021-09-25 发布日期:2021-09-29
通讯作者: *郑玉才（1965-）,男,内蒙古自治区呼伦贝尔人,博士,教授,研究方向为泌乳生物化学、分子遗传学。
作者简介:赵梦波（1995-）,男,河南焦作人,硕士,研究方向为泌乳生物化学;金素钰（1965-）,女,四川成都人,高级实验师,研究方向为乳品分析;黄林（1979-）,男,湖北黄冈人,硕士,讲师,研究方向为分子遗传学。
基金资助:
研究生创新型科研项目（CX2020SP104）

Genotyping on β-caseins of Jiulong Tibetan Yellow Cattle and Jiulong Yak

ZHAO Mengbo, JIN Suyu, HUANG Lin, ZHENG Yucai

College of Animal and Veterinary Sciences,Southwest Minzu University,Chengdu Sichuan 610041

Online:2021-09-25 Published:2021-09-29

摘要/Abstract

摘要： 为比较九龙藏黄牛和九龙牦牛β-酪蛋白（β-CN）的遗传变异体,试验采用酸性尿素聚丙烯酰胺凝胶电泳分析了九龙藏黄牛（n=42）和九龙牦牛（n=17）β-CN的基因型。结果表明,在九龙藏黄牛、九龙牦牛的β-CN中共检测到4 种等位基因,包括A1、A2、B、C,其中在九龙藏黄牛中有7 种基因型：A1A1、A1A2、BB、A1B、A2B、A1C、BC,优势等位基因为A1（频率0.702 4）,优势基因型为A1A1（频率0.547 6）;在九龙牦牛样本中有2 种基因型：A1A2、A2A2,优势等位基因为A2（频率0.764 7 ）。试验表明,九龙藏黄牛与九龙牦牛β-CN均表现出多态性,但优势等位基因明显不同。

关键词: 藏黄牛, 牦牛, β-酪蛋白, 酸性尿素电泳, 基因型

Abstract: The β-casein （β-CN） variants were identified in Jiulong Tibetan Yellow cattle and Jiulong yak by acid urea polyacrylamide gel electrophoresis. The results indicated that four alleles, including A1, A2, B and C, were detected in β-CN of Jiulong Tibetan Yellow cattle and Jiulong yak. There were 7 genotypes in Jiulong Tibetan yellow cattle： A1A1, A1A2, BB, A1B, A2B, A1C and BC, the dominant allele was A1 （the frequency was 0.702 4）, and the dominant genotype was A1A1 （the frequency was 0.547 6）. There were two genotypes in Jiulong yak： A1A2 and A2A2, and the dominant allele was A2 （the frequency was 0.7647）. This study showed that the β-CN polymorphisms were found in both Jiulong Tibetan yellow cattle and Jiulong yak, but the dominant alleles were significantly different.

Key words: Tibetan yellow cattle, Yak, β-casein, acid urea electrophoresis, genotype

赵梦波, 金素钰, 黄林, 郑玉才. 九龙藏黄牛和九龙牦牛β-酪蛋白基因型分析[J]. 中国乳业, 2021, 0(9): 120-124.

ZHAO Mengbo, JIN Suyu, HUANG Lin, ZHENG Yucai. Genotyping on β-caseins of Jiulong Tibetan Yellow Cattle and Jiulong Yak[J]. China Dairy, 2021, 0(9): 120-124.

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九龙藏黄牛和九龙牦牛β-酪蛋白基因型分析

Genotyping on β-caseins of Jiulong Tibetan Yellow Cattle and Jiulong Yak

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