关键词: 牛羊硬蜱, 蜱种鉴定, 微小扇头蜱, 长角血蜱, 蜱传梨形虫

Abstract: [Objective] This study aimed to explore the tick species on the body surface of cattle and sheep in Yinan county Shandong Province,and detect the prevalence of parasitic tick-borne pyriformis..[Method] Hard ticks were collected from the surface of cattle and sheep,and their morphological characteristics were preliminarily identified.Subsequently,molecular identification was carried out using the mitochondrial cox1 gene,and the 18S rRNA gene was used as the target gene to investigate the prevalence of parasitic tick-borne pyriformis.[Result] A total of 338 ticks were collected,including 219 longhorn blood ticks and 119 microfan-headed ticks. The COX1 gene sequences of five longhorn blood ticks showed the highest homology （99.7%~99.9%） with known species, while five microfan-headed ticks exhibited the highest similarity （99.3%~99.6%） to known species, all belonging to branch B. PCR analysis revealed an average detection rate of 7.10% （24/338） for Babesia and 3.25% （11/338） for Tyleria in longhorn blood ticks. [Conclusion] The results indicated that longhorn blood ticks and microfan-headed ticks are the dominant surface ticks on cattle and sheep in Yinan County,with common carriage of Paragonimiasis. Local authorities and livestock farming enterprises should enhance tick detection and control measures.

Key words: hard ticks of cattle and sheep, tick species identification, microscopic fan-headed tick, long-horned blood tick, tick-borne piroplasm