中国乳业 ›› 2026, Vol. 0 ›› Issue (3): 98-106.doi: 10.12377/1671-4393.26.03.14

• 质量安全 • 上一篇    下一篇

双峰驼原料奶及其养殖环境的真菌多样性分析研究

潘伊微1,2, 蔡树东1, 王万兴1, 克热木·司马义1, 张梦圆1, 李莲瑞2, 罗生金1,*   

  1. 1.哈密市动物疫病预防控制中心,新疆哈密 839000;
    2.农业农村部环塔里木畜草资源利用重点实验室,新疆阿拉尔 843300
  • 出版日期:2026-03-25 发布日期:2026-04-09
  • 通讯作者: *罗生金(1973-),男,甘肃民勤人,硕士,研究员,研究方向为畜牧业科技推广与动物临床疾病诊治。
  • 作者简介:潘伊微(1988-),女,陕西汉中人,硕士,高级畜牧师,研究方向为畜牧业技术推广;蔡树东(1991-),男,甘肃武威人,硕士,畜牧师,研究方向为畜牧渔业技术推广;王万兴(1994-),男,甘肃白银人,硕士,畜牧师,研究方向为家畜繁育技术推广工作;克热木·司马义(1976-),男,新疆哈密人,专科,畜牧师,研究方向为畜牧良种繁育;张梦圆(1994-),女,新疆哈密人,硕士,畜牧师,研究方向为畜牧业技术推广;李莲瑞(1968-),女,新疆阿拉尔人,博士,教授,研究方向为病原微生物及免疫学。
  • 基金资助:
    哈密市兴农人才扶持工程培养项目(2024XNRC009); 新疆“天山英才”培养计划“三农”骨干人才项目(2023SNGGNT030)

Analysis of Fungal Diversity in Raw Milk of Double-humped Camels and Their Breeding Environment

PAN Yiwei1,2, CAI Shudong1, WANG Wanxing1, Keremu Simayi1, ZHANG Mengyuan1, LI Lianrui2, LUO Shengjin1,*   

  1. 1. Hami Center for Animal Disease Control and Prevention,Hami Xinjiang 839000;
    2. Key Laboratory of Livestock and Forage Resources Utilization around Tarim,Ministry of Agriculture and Rural Affairs,Alar Xinjiang 843300
  • Online:2026-03-25 Published:2026-04-09

摘要: [目的]本研究通过对双峰驼原料奶及养殖环境中真菌群落进行多样性分析,明确其群落结构与分布特征,探究真菌对驼乳品质的影响,为保障驼乳质量安全、优化养殖环境提供科学依据。[方法]本试验分别采集3 家骆驼养殖合作社的骆驼原料奶奶样、暂存罐奶样、奶罐车奶样,饲料、饲草、垫土各6 组样本,采用ITS 1扩增子测序技术对驼乳组及环境组样本真菌群落结构及多样性进行分析。[结果]18 组样本共获得5 476 597 条原始序列,4 580 307 条有效序列,80 251 个 ASVs。原料奶中主要优势真菌属为Acrogenospora,其次为Trichosporon,环境组中饲料的优势菌属是Aschersonia,饲草的优势菌属是Aspergillus,垫土的优势菌属是Acrogenospora,驼奶组与环境组样本中真菌优势菌门为Ascomycota、Basidiomycota,与驼乳组相比较,环境组真菌多样性差异显著(P<0.05),不同采样点真菌群落结构存在一定差异,且菌群结构与丰度明显不同。[结论]3 个骆驼养殖合作社的驼乳及环境真菌多样性差异显著,部分真菌可降解驼乳中的蛋白质与脂肪从而影响奶品质。驼乳真菌群落组成与养殖环境密切相关,存在交叉污染风险,因此,加强环境管控与真菌监测可有效保障驼乳质量安全。

关键词: 双峰驼, 原料奶, 真菌, 多样性

Abstract: [Objective] This study conducted a diversity analysis of the fungal communities in raw milk of the bactrian camel and the environment where they are raised. The aim was to clarify the structure and distribution characteristics of the fungal communities,explore the impact of fungi on the quality of camel milk,and provide scientific basis for ensuring the quality and safety of camel milk and optimizing the breeding environment. [Method] In this experiment,raw camel milk samples,temporary storage tank milk samples,and milk tanker milk samples from camels,as well as six sets of samples each for feed,forage,and bedding soil,were collected from three camel breeding cooperatives. The ITS 1 amplification sequencing technology was used to analyze the fungal community structure and diversity of the camel milk group and environmental group samples. [Result] A total of 5 476 597 raw sequences and 4 580 307 valid sequences were obtained from 18 sample. 80 251 ASVs were identified. The main dominant fungal genera in raw milk samples was Acrogenospora,followed by Trichosporon. In the environmental group,the dominant genus was Aschersonia,in the forage was Aspergillus,and in the soil pad was Acrogenospora. In the camel milk group and the environmental group,the dominant fungal phyla were Ascomycota and Basidiomycota. Compared with the camel milk group,the fungal diversity in the environmental group showed significant differences(P<0.05). There were certain differences in the fungal community structure among different sampling sites,and the community structure and abundance were significantly different. [Conclusion] There were significant differences in the diversity of camel milk and environmental fungi among the three camel breeding cooperatives. Some fungi could degrade the proteins and fats in camel milk,thereby affecting the quality of the milk. The composition of the camel milk fungal community is closely related to the breeding environment and there is a risk of cross-contamination. Therefore,trengthening environmental control and fungal monitoring can effectively ensure the quality of camel milk.

Key words: Camelus bactrianus, raw milk, fungus, diversity

[1] Shephard,G.S.Impact of mycotoxins on human health in developing countries[J].Food Additives & Contaminants,2008,25(2):146-151.
[2] Garnier L,Valence F,Mounier J.Diversity and control of spoilage fungi in dairy products:an update[J].Microorganisms(Basel),2017,5(3):42.
[3] Delavenne E,Cliquet S,Trunet C,et al.Characterization of the antifungal activity of Lactobacillus harbinensis K.V9.3.1.Np and Lactobacillus rhamnosus K. C8.3.1I in yoghurt[J].Food Microbiology,2015(45):10-17.
[4] 刘继超,宋思青,胡聚峰,等.污染原料乳的微生物多样性和溯源研究[J].中国乳品工业,2025(8):39-44
[5] Garnier,L.; Valence,F.; Mounier,J.Diversity and Control of Spoilage Fungi in Dairy Products:An Update[J].Microorganisms(Basel),2017,5(3):42.
[6] El-Kadi S M,Habib A A,Elfadaly H,et al.Role of Fungal Enzymes in the Biochemistry of Egyptian Ras Cheese during Ripening Period[J].Open Access Library Journal,2015,2(8):1819-1832.
[7] 李丹蕾,孔夏冰,于悦,等.驼乳营养成分分析与真实性鉴别研究进展[J].食品科学,2025(10):346-357.
[8] 潘伊微,李文,罗生金.骆驼奶的营养价值与保健功能研究进展[J].黑龙江动物繁殖,2024,32(5):41-46.
[9] 赵恒,陈富安,赵慧君,等.不同地区花色腐乳真菌多样性分析[J].中国调味品,2022,47(10):57-61
[10] Quintana Á Rafael,Perea J M,García-Béjar B,et al.Dominant Yeast Community in Raw Sheep's Milk and Potential Transfers of Yeast Species in Relation to Farming Practices[J].Animals(Basel),2020,10(5):906.
[11] 霍路曼,张晓利,陈彦丽,等.生鲜乳样品及环境中微生物多样性研究[J].中国乳品工业,2024,52(10):43-46,63.
[12] Zhang Y,Xu J,Ding F,et al.Multidimensional profiling indicates the shifts and functionality of wheat-origin microbiota during high-temperature Daqu incubation[J].Food Research International,2022(156):111191.
[13] Quigley L,O'Sullivan O,Stanton C,et al.The complex microbiota of raw milk[J].FEMS Microbiology Reviews,2013,37(5):664-698.
[14] Kandasamy S,Park W S,Yoo J,et al.Characterisation of fungal contamination sources for use in quality management of cheese production farms in Korea[J].Animal Bioscience,2020,33(6):1002-1011.
[15] Bernardi A O,Stefanello A,Garcia M V,et al.The control of cheese and meat product spoilage fungi by sanitizers:In vitro testing and food industry usage[J].Food Science & Technology,2021(144):111204.
[16] 邵泽远,王舒淇,刘德江,等.一株产脂肪酶真菌的筛选、培养条件优化及脂肪酶结构特征[J].微生物学报,2025,65(10):4472-4487.
[17] Ider S,Belguesmia Y,Coucheney F,et al.Impact of seasonality and environmental conditions on yeast diversity from camel's milk collected in Algeria[J].Archives of Microbiology,2019,201(3):399-407.
[18] 朱丽,刘玮,何宇星,等.赤峰市巴林右旗地区乳及传统发酵乳制品中真菌多样性及其功能预测分析[J].现代食品科技,2025,41(9):86-94.
[19] 芦文娟,李宝坤,卢士玲,等.新疆塔城牧区原料乳中酵母菌的分离鉴定[J].食品与生物技术学报,2018,37(12):1284-1291.
[20] Barone G D,Emmerstorfer-Augustin A,Biundo A,et al.Industrial Production of Proteins with Pichia pastoris—Komagataella phaffii[J]. Biomolecules(Basel,Switzerland),2023,13(3):441.
[21] 王新月. 青海地区自然发酵酸牦牛奶风味成分及真菌多样性分析[D].天津:天津科技大学,2023.
[22] 刘辉放,何志敏,孙守江,等.地顶孢霉培养物在提高牛乳营养和风味口感中的应用[J].中国乳业,2017(5):43-46.
[23] 李洋,窦秀静,王一臻,等.地顶孢霉培养物对奶牛体细胞数与乳中病原菌的影响[A]中国奶业协会.第七届中国奶业大会论文集[C].哈尔滨:东北农业大学动物科学技术学院,2016.
[24] Fuchs T,Melcher F,Rerop Z S,et al.Identifying carbohydrate-active enzymes of Cutaneotrichosporon oleaginosus using systems biology[J].Microbial Cell Factories,2021,20(1):205-218.
[25] 涂洁. 座壳孢菌酯酶和脂肪酶的纯化及酶学性质的研究[D].福州:福建农林大学,2014.
[26] Corzo-León D E,MacCallum D M,Munro C A.Host Responses in an Ex Vivo Human Skin Model Challenged With Malassezia sympodialis[J].Frontiers in Cellular and Infection Microbiology,2021(10):561382.
[27] Rampanti G,Ferrocino I,Harasym J,et al.Queijo Serra da Estrela PDO Cheese:Investigation into Its Morpho-Textural Traits,Microbiota,and Volatilome[J].Foods,2022,12(1):169.
[1] 杨波, 栗慧, 王青山, 武二斌, 魏东. 基于培养法与16SrRNA测序的华北地区规模化牧场原料乳嗜冷菌多样性综合分析[J]. 中国乳业, 2026, 0(2): 87-95.
[2] 李文, 潘伊微, 木塔力甫·阿迪力, 罗生金. 哈密市舍饲双峰驼泌乳期泌乳量及乳成分规律研究[J]. 中国乳业, 2025, 0(6): 53-58.
[3] 李永青, 蔡扩军, 王涛, 孙鹏亮, 徐敏, 陆东林. 内蒙古与新疆双峰驼乳化学成分特征比较分析[J]. 中国乳业, 2025, 0(5): 107-114.
[4] 蔡树东, 潘伊微, 李文, 罗生金. 中国双峰驼驼乳营养价值研究进展[J]. 中国乳业, 2025, 0(12): 109-117.
[5] 周玲, 徐广新. 乳制品中真菌控制研究进展[J]. 中国乳业, 2024, 0(7): 88-91.
[6] 蔡扩军, 陆东林, 何晓瑞, 朱建忠, 徐敏. 双峰驼乳宏量营养素含量及生驼乳标准中相关指标商榷[J]. 中国乳业, 2024, 0(11): 157-162.
[7] 何晓瑞, 殷娜, 徐敏, 李景芳, 陆东林. 新疆驼乳粉中维生素含量测定的研究[J]. 中国乳业, 2021, 0(6): 83-87.
[8] 徐敏, 殷娜, 何晓瑞, 李景芳, 陆东林. 新疆驼乳粉氨基酸含量和组成分析[J]. 中国乳业, 2021, 0(3): 77-80.
[9] 林少华, 罗红霞. 下一代测序技术在乳业中的应用[J]. 中国乳业, 2020, 0(4): 79-83.
[10] 吴建懿. 硫氰酸钠在婴幼儿配方奶粉中的技术分析[J]. 中国乳业, 2020, 0(12): 47-49.
[11] 王东杰, 王礞礞. 2019年全球奶源与贸易形势回顾与展望[J]. 中国乳业, 2020, 0(1): 14-16.
[12] 徐自奥, 徐娟娟, 卢占法, 曹赞, 李晓祥. 虫草欣康对奶牛繁殖性能的影响[J]. 中国乳业, 2019, 0(7): 33-35.
[13] 邬玎岚, 古焕群, 林健汝, 李飞鸣. 基于供应链的液态乳制品生产流程研究[J]. 中国乳业, 2019, 0(6): 59-62.
Viewed
Full text


Abstract

Cited

  Shared   
  Discussed   
No Suggested Reading articles found!