微生物试管法测定叶酸含量的方法研究

doi:10.12377/1671-4393.22.07.10

中国乳业 ›› 2022, Vol. 0 ›› Issue (7): 44-48.doi: 10.12377/1671-4393.22.07.10

微生物试管法测定叶酸含量的方法研究

胡淑红

绿城农科检测技术有限公司,浙江杭州 310000

出版日期:2022-07-25 发布日期:2022-08-02
作者简介:胡淑红（1990-）,女,浙江金华人,本科,中级工程师,研究方向为食品营养物质检测与方法。

Study on the Method for Determination of Folic Acid Content by Microbial Test Tube Method

HU Shuhong

Greentown Agricultural Testing Technology Co.,LTD.,Hangzhou Zhejiang 310000

Online:2022-07-25 Published:2022-08-02

摘要/Abstract

摘要： 叶酸是鼠李糖乳杆菌生长的必需营养素,微生物法检测叶酸是利用鼠李糖乳杆菌生长速度与叶酸的含量相关性建立的。鉴于微生物法试验周期长,菌种传代保藏比较繁琐,该法是基于《GB 5009.211—2014食品安全国家标准食品中叶酸的测定》方法,改进了乳酸杆菌接种液的制备方法和叶酸培养液的培养时间。本文通过分析方法的标准曲线的线性、精密度、准确度和方法稳定性来验证优化方法的适用性。该方法标准曲线线性关系良好（R²>0.99）,回收率>90%,精密度和准确度好,操作简便,缩短了试验周期,提高了检测效率,为改进叶酸检测方法提供了依据。

关键词: 叶酸, 试验周期, 线性, 精密度, 准确度

Abstract: Folic acid is an essential nutrient for the growth of Lactobacillus rhamnosus,The microbial assay of folic acid was established by using the correlation between the growth rate of Lactobacillus rhamnosus and the content of folic acid.In view of the long test cycle of microbial method and the cumbersome preservation of bacteria,this method is based on the method of GB 5009.211-2014 National Food Safety Standard Determination of Folic acid in Food,improving the preparation method of lactobacillus inoculant and the culture time of folic acid culture liquid.This paper verified the applicability of the optimization method by analyzing the linearity of the standard curve,precision,accuracy and stability of the method．This method has good curve relation （R²> 0.99）,good recovery > 90%,good precision and accuracy,simple operation,shortens test period,improves detection efficiency,and provides a basis for improving the detection method of folic acid．

Key words: folic acid, test period, linear, precision, accuracy

中图分类号:

TS252.51

胡淑红. 微生物试管法测定叶酸含量的方法研究[J]. 中国乳业, 2022, 0(7): 44-48.

HU Shuhong. Study on the Method for Determination of Folic Acid Content by Microbial Test Tube Method[J]. China Dairy, 2022, 0(7): 44-48.

参考文献

[1] 中华人民共和国卫生部.GB 10765—2010 食品安全国家标准婴儿配方食品[S].
[2] 中华人民共和国卫生部.GB 10767—2010 食品安全国家标准较大婴儿和幼儿配方食品[S].
[3] 林新城,强桂彦.中国育龄期女性MTHFR、MTRR基因型与出生缺陷对沧州地区叶酸增补的借鉴分析[J].公共卫生与预防医学,2019,30(6):80-84.
[4] Kang W,Chen Y,Lu D,et al.Folic acid contributes toperipheral nerve injury repair by promoting schwann cellproliferation,migration,and secretion of nerve growthfactor[J].Neural RegenerationResearch,2019,14(1):132-139.
[5] 李永男,冒鑫娥,薛慧萍.叶酸与阿尔兹海莫病相关性的Meta分析[J].护理研究,2020,34(2):212-218.
[6] 潘凤瑜,周玲,木拉力别克.黑扎提.新疆农牧人群叶酸缺乏情况及与高血压的相关性[J].海南医学院学报,2019,25(23):1780-1786.
[7] 黄进丽,杨祖伟,陈叶兰.微生物法测定叶酸、生物素、维生素B₁₂接种液制备方法优化[J].食品安全质量检测学报,2020(1):99-105.
[8] 周敏,宁礼信,高志城,等.微生物法测定维生素矿物片中的叶酸含量[J].食品科技,2020(8):253-258.

Metrics

Viewed

Full text

Abstract

Cited

Shared

Discussed

微生物试管法测定叶酸含量的方法研究

Study on the Method for Determination of Folic Acid Content by Microbial Test Tube Method

PDF (PC)

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 4

Metrics

本文评价

推荐阅读 0

[1]	吴思敏, 严家俊, 黄钟标. 微生物法测定婴幼儿配方乳粉中叶酸含量不确定度评估[J]. 中国乳业, 2021, 0(7): 91-97.
[2]	苏妙仪, 严家俊, 张娟. 微生物法检测乳粉中叶酸的优化研究[J]. 中国乳业, 2021, 0(2): 81-85.
[3]	崔承远. 婴幼儿配方奶粉中丁酸、己酸、辛酸、癸酸的测定方法验证[J]. 中国乳业, 2021, 0(12): 105-110.
[4]	胡淑红, 王荣艳. 富含乳脂肪球膜蛋白乳清粉中总磷脂的含量测定方法研究[J]. 中国乳业, 2020, 0(8): 73-76.