纤溶酶检测中底物配制试剂的优化研究——基于MTB缓冲液与超纯水的比较

doi:10.12377/1671-4393.26.03.10

中国乳业 ›› 2026, Vol. 0 ›› Issue (3): 68-72.doi: 10.12377/1671-4393.26.03.10

纤溶酶检测中底物配制试剂的优化研究——基于MTB缓冲液与超纯水的比较

陈茂姣, 何刚, 邓燕琴

四川新希望乳业有限公司洪雅阳平分公司,四川眉山 620360

出版日期:2026-03-25 发布日期:2026-04-09
作者简介:陈茂姣（1993-）,女,四川眉山人,本科,助理工程师,研究方向为食品安全检测;何刚（1985-）,男,四川眉山人,本科,助理工程师,研究方向为食品科学与工程;邓燕琴（1990-）,女,四川峨眉人,本科,工程师,研究方向为乳品检测。

Optimization of Substrate Preparation Reagents for Plasmin Detection--A Comparative Study of MTB Buffer and Ultrapure Water

CHEN Maojiao, HE Gang, DENG Yanqin

Hongya Yangping Branch,Sichuan New Hope Dairy Co.,Ltd.,Meishan Sichuan 620360

Online:2026-03-25 Published:2026-04-09

摘要/Abstract

摘要： [目的]本研究旨在探究不同底物配制试剂对乳制品中纤溶酶检测结果的影响,以建立一种准确、稳定的检测方法。[方法]通过底物显色法,比较超纯水和MTB缓冲液配制纤溶酶底物的效果。[结果]MTB缓冲液在吸光度平行性、加标回收率（113%~126%）和酶活力测定值上均优于超纯水（加标回收率141%~152%,超出标准范围）。MTB缓冲液能够提供适宜的pH值（7.6±0.1）和稳定的离子环境,显著提高检测的准确性和重复性。[结论]本研究为乳制品中纤溶酶的精准检测提供优化方案,对提升乳制品质量控制水平具有指导意义。

关键词: 纤溶酶, 酶标仪, MTB缓冲液, 纤溶酶底物, 乳制品检测

Abstract: [Objective] This study aimed to investigate the effects of different reagents for substrate preparation on the detection of plasmin in dairy products,with the goal of establishing an accurate and stable detection method. [Method] A chromogenic substrate method was employed to compare the effects of using ultrapure water versus MTB buffer for plasmin substrate preparation. [Result] The MTB buffer group demonstrated superior performance in terms of absorbance parallelism,spike recovery rate（113%~126%）,and plasmin activity measurements compared to the ultrapure water group （spike recovery rate 141%~152%,exceeding the standard range）.The MTB buffer provided an optimal pH （7.6±0.1）and a stable ionic environment,significantly improving the accuracy and repeatability of the detection. [Conclusion] This study offered an optimized solution for the precise detection of plasmin in dairy products,providing guidance for enhancing quality control in the dairy industry.

Key words: plasmin, microplate reader, MTB buffer, plasmin substrate, dairy product detection

陈茂姣, 何刚, 邓燕琴. 纤溶酶检测中底物配制试剂的优化研究——基于MTB缓冲液与超纯水的比较[J]. 中国乳业, 2026, 0(3): 68-72.

CHEN Maojiao, HE Gang, DENG Yanqin. Optimization of Substrate Preparation Reagents for Plasmin Detection--A Comparative Study of MTB Buffer and Ultrapure Water[J]. China Dairy, 2026, 0(3): 68-72.

参考文献

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[6] Rauh V M,Johansen L B,Ipsen R,et al.Plasmin activity in UHT milk:Relationship between proteolysis,age gelation,and bitterness[J].Journal of Agricultural and Food Chemistry,2014,62(28):6852-6860.
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[8] 崔佳琦,杨俊,于鹏飞,等.不同贮藏温度下内源蛋白酶对原料乳品质影响的研究[J].中国乳品工业,2025,53(11):62-68.

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纤溶酶检测中底物配制试剂的优化研究——基于MTB缓冲液与超纯水的比较

Optimization of Substrate Preparation Reagents for Plasmin Detection--A Comparative Study of MTB Buffer and Ultrapure Water

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