微生物法检测特殊医学用途婴儿配方食品中叶酸含量的研究

doi:10.12377/1671-4393.23.06.13

中国乳业 ›› 2023, Vol. 0 ›› Issue (6): 68-72.doi: 10.12377/1671-4393.23.06.13

微生物法检测特殊医学用途婴儿配方食品中叶酸含量的研究

孙潇慧^1,2, 泮秋立^1,2, 霍胜楠^1,2, 钟立霞^1,2, 程祥龙^1,2, 李文婷³, 孟静^1,2,*

1 山东省食品药品检验研究院,山东济南 250101;
2 山东省特殊医学用途配方食品质量工程技术研究中心,山东济南 250101;
3 山东省医疗器械和药品包装检验研究院,山东济南 250101

出版日期:2023-06-25 发布日期:2023-06-30
通讯作者: ^*孟静（1979-）,女,河北晋州人,硕士,正高级工程师,研究方向为食品检验检测。
作者简介:孙潇慧（1990-）,女,山东嘉祥人,硕士,工程师,研究方向为食品质量与安全;泮秋立（1989-）,女,山东齐河人,硕士,工程师,研究方向为食品质量与安全;霍胜楠（1980-）,女,山东淄博人,博士,应用技术研究员,研究方向为食品检验检测及质量管理;钟立霞（1989-）,女,山东临朐人,硕士,工程师,研究方向为食品检验检测;程祥龙（1992-）,男,山东临沂人,硕士,工程师,研究方向为食品检验检测;李文婷（1990-）,女,山东菏泽人,本科,助理工程师,研究方向为医疗器械。

Microbiological Detection of Folic Acid Content in Infant Formula for Special Medical Purposes

SUN Xiaohui^1,2, PAN Qiuli^1,2, HUO Shengnan^1,2, ZHONG Lixia^1,2, CHENG Xianglong^1,2, LI Wenting³, MENG Jing^1,2,*

¹Shandong Institute for Food and Drug Control,Jinan Shandong 250101;
²Shandong Research Center of Engineering and Technology for Quality Control of Food for Special Medical Purposes,Jinan Shandong 250101;
³Shandong Institute of Medical Device and Pharmaceutical Packaging Inspection,Jinan Shandong 250101

Online:2023-06-25 Published:2023-06-30

摘要/Abstract

摘要： [目的]分析和探讨微生物方法在特殊医学用途婴儿配方食品中叶酸含量测定中的应用。[方法]利用微生物法对5 类不同基质特殊医学用途婴儿配方食品中叶酸含量及回收率进行检测,分析其精密度和准确度。[结果]5 类不同基质特殊医学用途婴儿配方食品中叶酸含量检测较稳定,加标回收率90.0%~108.8%,相对标准偏差1.05%~2.40%。[结论]微生物法检测特殊医学用途婴儿配方食品中叶酸含量具有较高的准确度,在符合国标的前提下,验证了标准的合理性,为我国特殊医学用途婴儿配方食品中叶酸含量测定提供科学参考。

关键词: 微生物法, 特殊医学用途婴儿配方食品, 叶酸

Abstract: [Objective]To analyze and explore the application of microbiological methods in the determination of folic acid in infant formula for special medical purposes.[Method]The content and recovery of folic acid in five kinds of infant formula with different substrates for special medical purposes were detected by microbiological method,and the precision and accuracy of the results were analyzed.[Result]The detection stability of folic acid in infant formula for special medical purposes with five different matrices is good.The recovery rate of standard addition is between 90.0%~108.8%.The relative standard deviation is 1.05%~2.40%.[Conclusion]Microbiological methods for detecting folic acid content in infant formula for special medical purposes have high sensitivity and accuracy. Under the premise of meeting the national standard,the rationality of the standard is verified,which provides a scientific reference for the determination of folic acid content in infant formula for special medical purposes in China.

Key words: microbiological method, infant formula for special medical purpose, folic acid

孙潇慧, 泮秋立, 霍胜楠, 钟立霞, 程祥龙, 李文婷, 孟静. 微生物法检测特殊医学用途婴儿配方食品中叶酸含量的研究[J]. 中国乳业, 2023, 0(6): 68-72.

SUN Xiaohui, PAN Qiuli, HUO Shengnan, ZHONG Lixia, CHENG Xianglong, LI Wenting, MENG Jing. Microbiological Detection of Folic Acid Content in Infant Formula for Special Medical Purposes[J]. China Dairy, 2023, 0(6): 68-72.

参考文献

[1] 市场监管总局:对“配方粉”事件涉事公司立案调查涉及宁波特壹、青岛金大洋-搜狐网https://m.sohu.com/a/319125937_120065756?_trans_=010004_pcwzy.
[2] 贾海先,李春雨,梁栋,等.我国已批准注册特殊医学用途配方食品营养成分含量及科学性比对分析[J].中国食品卫生杂志,2021,33(4):485-492.
[3] Singer P,Blaser A R,Berger M M,et al.ESPEN guideline on clinical nutrition in the intensive care unit[J].Clinical Nutrition,2019,38(1):48-79.
[4] Gomes F,Schuetz P,Bounoure L,et al.ESPEN guidelines on nutritional support for polymorbid internal medicine patients[J].Clinical Nutrition,2018,37(1):336-353.
[5] 李美英,李雅慧,姜雨,等.浅析我国特殊医学用途配方食品监管概况[J].食品工业科技,2016,37(18):387-390.
[6] 李雨哲,肖伟敏,杨俊,等.我国特殊医学用途配方食品配套检验方法标准现状及展望[J].中国食品卫生杂志,2021,33(5):610-615.
[7] 国家卫生和计划生育委员会. 特殊医学用途婴儿配方食品通则:GB25596-2010[S].
[8] 国家卫生和计划生育委员会,国家食品药品监督管理总局.食品安全国家标准特殊医学用途配方食品通则:GB 29922-2013[S].
[9] Gazzali A M,Lobry M,Colombeau L,et al.Stability of folic acid under several parameters[J].European Journal of Pharmaceutical Sciences,2016,93(10):419-430.
[10] Strandler H S,Patring J,Jägerstad M,et al.Challenges in the determination of unsubstituted food folates:Impact of stabilities and conversions on analytical results[J].Journal of Agricultural & Food Chemistry,2015,63(9):2367-2377.
[11] Crider K S,Bailey L B,Berry R J.Folic acid food fortification-Its history,effect,concerns,and future directions[J].Nutrients,2011,3(3):370-384.
[12] Blehaut H,Mircher C,Ravel A,et al.Effect of leucovorin(Folinic acid) on the developmental quotient of children with Down’s syndrome(Trisomy 21)and influence of thyroid status[J].Plos One,2010,5(1):8394.
[13] 康文怀,叶晓利,李慧,等.食品中叶酸分析方法及稳定性研究进展[J].河北科技大学学报,2019,40(5):446-453.
[14] Hanson A D,Gregory Ⅲ J F.Folate biosynthesis,turnover,and transport in plants[J].Annual Review of Plant Biology,2011,62(1):105-125.
[15] 李美英,邓少伟,李雅慧,等.我国特殊医学用途婴儿配方食品现状浅析[J].食品与生物技术学报,2021,40(05):104-111.
[16] 全国认证认可标准化技术委员会.GB/T 27404-2008《实验室质量控制规范食品理化检测》理解与实施[M].北京:中国标准出版社,2009.
[17] 许书道. 定量分析方法评价[J].化学分析计量,2002,11(1):30-31.
[18] 任成忠,毛丽芬.加标回收实验的实施及回收率计算的研究[J].工业安全与环保,2006,32(2):9-11.
[19] Prasad A S,Brewer G J.Essential and toxic trace elements and vitamins in human health[M].Salt Lake City:SUTERP M,2020:217-239.
[20] 张丽娟,蔡子微,范钦信.维生素B₁₂缺乏与老年智力障碍[J].国外医学:生理病理科学与临床分册,1998,18(1):91-93.
[21] Liu R R,Mi B B,Zhao Y L,et al.Effect of B vitamins from diet on hypertension[J].Archives of Medical Research,2017,48(2):187-194.
[22] AOAC. Official methods of analysis of the association of official analytical chemists[Z].
[23] The United States Pharmacopoeia Committee. United States Pharmacopeia[Z].

Metrics

Viewed

Full text

Abstract

Cited

Shared

Discussed

微生物法检测特殊医学用途婴儿配方食品中叶酸含量的研究

Microbiological Detection of Folic Acid Content in Infant Formula for Special Medical Purposes

PDF (PC)

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 6

Metrics

本文评价

推荐阅读 0

[1]	胡淑红. 微生物试管法测定叶酸含量的方法研究[J]. 中国乳业, 2022, 0(7): 44-48.
[2]	巴宁宁. 特殊医学用途婴儿配方食品冲调温度的研究分析[J]. 中国乳业, 2022, 0(5): 84-88.
[3]	严家俊, 姚丽锋, 綦艳, 张娟, 黄翠莉, 吴思敏. 基于新国标的两种微生物法测定乳粉中叶酸的比对研究[J]. 中国乳业, 2022, 0(11): 76-80.
[4]	吴思敏, 严家俊, 黄钟标. 微生物法测定婴幼儿配方乳粉中叶酸含量不确定度评估[J]. 中国乳业, 2021, 0(7): 91-97.
[5]	苏妙仪, 严家俊, 张娟. 微生物法检测乳粉中叶酸的优化研究[J]. 中国乳业, 2021, 0(2): 81-85.
[6]	崔力航, 郭睿. 特殊医学用途婴儿配方食品营养成分调查分析[J]. 中国乳业, 2020, 0(6): 45-49.