中国乳业 ›› 2022, Vol. 0 ›› Issue (11): 76-80.doi: 10.12377/1671-4393.22.11.14

• 质量安全 • 上一篇    下一篇

基于新国标的两种微生物法测定乳粉中叶酸的比对研究

严家俊1, 姚丽锋2, 綦艳1, 张娟1, 黄翠莉1, 吴思敏1   

  1. 1 广东产品质量监督检验研究院,广东佛山 528300
    2 拱北海关技术中心,广东珠海 519015
  • 出版日期:2022-11-25 发布日期:2022-12-14
  • 作者简介:严家俊（1992-）,男,广东揭阳人,硕士,工程师,研究方向为食品及食品相关产品质量安全检测与风险评估;姚丽锋（1976-）,女,河南洛阳人,硕士,高级兽医师,研究方向为食源性致病微生物检测;綦艳（1978-）,女,山东青岛人,硕士,高级工程师,研究方向为食品及食品相关产品质量安全检测与风险评估;张娟（1981-）,女,山东淄博人,博士,高级工程师,研究方向为食品及食品相关产品质量安全检测与风险评估;黄翠莉（1983-）,女,山东济宁人,硕士,高级工程师,研究方向为食品及食品相关产品质量安全检测与风险评估;吴思敏（1992-）,女,广东揭阳人,本科,工程师,研究方向为食品及食品相关产品质量安全检测与风险评估。
  • 基金资助:
    国家市场监管总局科技计划项目（2020MK080）; 广东省质量技术监督局科技项目（2022CZ03）; 广州市科技计划项目（202102080652）; 海关总署科研项目（2020HK189）; 珠海市科技计划项目（ZH22036201210017PWC）

A Comparative Study on the Determination of Folic Acid in Milk Powder by Two Microbial Methods Based on the New National Standard

YanJiajun1, YAO Lifeng2, QI Yan1, ZHANG Juan1, HUANG Cuili1, WU Simin1   

  1. 1Guangdong Testing Institute for Product Quality Supervision,Foshan Guangdong 528300;
    2Technical Center of Gongbei Customs,Zhuhai Guangdong 519015
  • Online:2022-11-25 Published:2022-12-14

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摘要： [目的]对即将实施的《GB5009.211—2022 食品安全国家标准食品中叶酸的测定》中两种微生物法测定乳粉中叶酸进行比对研究。[方法]验证试管法和微孔板法测定叶酸的准确度和重复性,根据数据的差异与试验操作过程对两种方法进行比较分析。[结果]由准确性试验可知,两种方法所测结果均与标准物质真值相符,相对标准偏差（RSD）分别为2.7%和2.9%,无明显差异。由重复性试验结果可知试管法RSD值为1.3%~3.1%,微孔板RSD值为1.6%~2.9%。[结论]新国标的两种微生物法的准确性和重复性都较好,均能对乳粉中叶酸进行有效测定;微孔板法效率更高,成本更低,适用于大批量样品的检测需求。

关键词: 叶酸, 乳粉, 试管法, 微孔板法, 新国标

Abstract: [Objective]This study compared two microbial methods for the determination of folic acid in milk powder in the upcoming GB5009.211-2022 National Food Safety Standard for the Determination of Folic Acid in Food. [Method]The accuracy and repeatability of the test tube method and the microplate method for the determination of folic acid were verified,and the two methods were compared and analyzed according to the difference of the data and the experimental operation process.[Result]It can be seen from the accurate experiment that the measured results of the two methods are consistent with the true value of the reference material,and the relative standard deviation（RSD）is 2.7% and 2.9%,respectively,with no significant difference. From the consequences of repeatability experiments,it can be known that the RSD value of the test tube method is 1.3% to 3.1%, and the RSD value of the microplate is 1.6% to 2.9%.[Conclusion]The accuracy and repeatability of the two microbiological methods of the new national standard are good,and both can effectively determine folic acid in milk powder. The microplate method has higher efficiency and lower cost,and is appropriate for the detection needs of large batches of samples.

Key words: folic acid, milk powder, test tube method, microplate method, new national standard

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