关键词: 生乳, 黄曲霉毒素M₁, 胶体金免疫层析法, 酶联免疫吸附法

Abstract: [Objective] To analyze the reproducibility, recovery, precision of ELISA（enzyme-linked immunosorbent assay） for the determination of aflatoxin M₁ residues in raw milk,as well as the echo between colloidal gold immunochromatography and its results,and the feasibility of the combined use of the two methods. [Method]Aflatoxin M₁ in raw milk samples was detected with colloidal gold rapid test strips,and aflatoxin M₁ ELISA kit（the lower limit of detection in fresh milk was 0.018 μg/kg） was used for control experiments.[Result]The colloidal gold strip method showed an echelon effect on the detection results of different concentrations of aflatoxin M₁ residue in raw milk,and could echo the quantitative detection results of ELISA.The results of ELISA assay for the quantification of different concentrations of aflatoxin M₁ residue in raw milk showed good reproducibility and high stability,and the recovery rate was between 94.83% and 100.53%,and the RSD ranging from 1.11% to 3.01%.[Conclusion]The determination of aflatoxin M₁ in raw milk by two rapid detection methods was convenient and rapid,and the results were stable and accurate with high reliability.It can be used as a basic data reference for the daily rapid acceptance of raw milk in dairy enterprises.

Key words: raw milk, aflatoxin M₁, colloidal gold immunoassay, ELISA