液相色谱-单四级杆法检测奶粉中泛酸的应用

doi:10.12377/1671-4393.22.07.13

中国乳业 ›› 2022, Vol. 0 ›› Issue (7): 59-64.doi: 10.12377/1671-4393.22.07.13

液相色谱-单四级杆法检测奶粉中泛酸的应用

徐旖¹, 王荣艳¹, 陈青俊¹, 任一平^2,*

1 浙江公正检验中心有限公司,浙江杭州 310051;
2 浙江清华长三角研究院,浙江嘉兴 314000

出版日期:2022-07-25 发布日期:2022-08-02
通讯作者: ^*任一平（1952-），男，浙江嘉兴人，本科，教授，高级工程师，研究方向为食品安全检验技术。
作者简介:徐旖（1995-），女，浙江衢州人，本科，助理工程师，研究方向为食品检验技术；王荣艳（1982-），女，河北邢台人，硕士，高级工程师，研究方向为食品检验技术；陈青俊（1970-），男，浙江杭州人，本科，高级工程师，研究方向为食品检验技术。

Application of Pantothenic Acid in Milk Powder by Liquid Chromatography-single Quadrupole Method

XU Yi¹, WANG Rongyan¹, CHEN Qingjun¹, REN Yiping^2,*

¹Zhejiang Fair Inspection Center Co.,Ltd.,Hangzhou Zhejiang 310051;
²Zhejiang Tsinghua Yangtze River Delta Research Institute,Jiaxing Zhejiang 314000

Online:2022-07-25 Published:2022-08-02

摘要/Abstract

摘要： 随着水解乳清蛋白原料在婴幼儿食品中的开发利用,给奶粉中泛酸检测造成了巨大的困难。开发本方法旨在解决水解蛋白对检测的干扰问题。奶粉样品经温水溶解、调节pH值、沉淀蛋白后,采用HSS T3液相色谱柱法分离,以10 mmol/L乙酸铵溶液（含0.5%甲酸）和甲醇为流动相进行梯度洗脱,电喷雾离子化后由单四极杆质谱检测,同位素内标法定量。结果表明：方法定量限为0.0141 mg/100 g,线性范围内低、中、高3 个标准添加水平的回收率为92.0%~102.7%,相对标准偏差在0.31%~2.23%;本检验方法与国标法对于羊奶粉、牛奶粉基质样品中泛酸成分的测定结果无显著差异。该方法操作过程简单、分析周期短、灵敏度高、重复性好,适用于特殊医学配方奶粉、婴幼儿配方奶粉中泛酸的测定,尤其对添加水解蛋白的奶粉样品具有理想的定量效果,具有极大应用前景。

关键词: 液相色谱-单四极杆质谱, 泛酸, 水解蛋白

Abstract: With the development and utilization of hydrolyzed whey protein raw materials in infant food,it has caused huge difficulties in the detection of pantothenic acid in milk powder. This method was developed to solve the problem of the interference of hydrolyzed proteins on the detection. The milk powder sample was dissolved in warm water,adjusted pH value,and precipitated protein,and was separated by HSS T3 liquid chromatography column method,detected by single quadrupole mass spectrometry after spray ionization,isotope internal standard method for quantification. The results showed that the limit of quantification of the method was 0.0141 mg/100 g,the recoveries of the low,medium and high standard addition levels in the linear range were 92.0%~102.7%,and the relative standard deviations were 0.31%~2.23%. There was no significant difference in the determination results of pantothenic acid in goat milk powder and milk powder matrix samples by the national standard method. The method has simple operation process,short analysis period,high sensitivity and good repeatability,is suitable for the determination of pantothenic acid in special medical formula milk powder and infant formula milk powder,especially for milk powder samples with added hydrolyzed protein,has great prospect.

Key words: liquid chromatography-single quadrupole mass spectrometry（QDA）, pantothenic acid, hydrolyzed protein

中图分类号:

TS252.7

徐旖, 王荣艳, 陈青俊, 任一平. 液相色谱-单四级杆法检测奶粉中泛酸的应用[J]. 中国乳业, 2022, 0(7): 59-64.

XU Yi, WANG Rongyan, CHEN Qingjun, REN Yiping. Application of Pantothenic Acid in Milk Powder by Liquid Chromatography-single Quadrupole Method[J]. China Dairy, 2022, 0(7): 59-64.

参考文献

[1] 中华人民共和国卫生部. GB 14880—2012 食品营养强化剂使用标准[S].
[2] 中华人民共和国国家卫生和计划生育委员会. GB 29922—2013 特殊医学配方食品通则[S].
[3] 中华人民共和国国家卫生和计划生育委员会. GB5009.210—2016 食品中泛酸的测定[S].
[4] Aanyal G.Methods for microbiological analysis of selectednutrients[J]. Journal of AOAC International,1996,8(1): 41-45.
[5] 张旭,马妮,郑洪. 微生物法测定食品中泛酸的含量[J].中国微生态学杂志,2012,24(7):654-655.
[6] 林丽琴,杨直,石云峰. HPLC测定泛酸钙的有关物质[J]. 中国现代应用药学,2012,29(11):10135-1038.
[7] 杜彦山,张志国,贾云虹,等. 高效液相色谱法测定奶粉中泛酸[J]. 食品研究与开发,2007,28(6):121-124.
[8] 刘志楠,喻东威,宋晓东,等. 烟酸和泛酸不同方法检测的对比[J]. 食品研究与开发,2011,32(11):90-93.
[9] 杨发树,刘耀敏,陈照. 反相高效液相色谱法测定预混合饲料中的D-泛酸[J]. 饲料研究,2012,35(8):73-75.
[10] 刘志楠,喻东威,赵源,等.牛奶中泛酸含量测定[J]. 食品科学,2012,33(2):177-180.
[11] 李少旦,彭卫芳.反相高效液相色谱法同时测定维生素B₆、烟酰胺和泛酸钙[J]. 理化检验:化学分册,2009,45(7):800-802.
[12] Pakin C,Bergaentzle M, Hubscher V,et al.Fluorimetricdetermination of pantothenic acid in foods by liquid chromatographywith post-column derivatization[J]. Journal of Chromatography A,2004,1035(1):87-95.
[13] Haughey S A,O'kane A A,Baxter G A,et al.Determinationof pantothenic acid in foods by optical biosensor immunoassay[J]. Journal of AOAC International,2005,88(4):1008-1014.
[14] Rychlik M.Quantification of free and bound pantothenic acid in foods and blood plasma by a stable isotope dilution assay[J]. Journal of Agricultural and Food Chemistry,2000,48(4):1175-1181.
[15] Banno K,Matsuoka M,Horimoto S,et al.Simultaneous determination of pantothenic acid and hopantenic acid in biological samples and natural products by gas chromatography-mass fragmentography[J]. Journal of Chromatography B,1990,525:255-264.
[16] Zhang Q,Qiu J,Liao N S.Simultaneous determination of calcium pantothenate and calcium-amino propionate and γ-butyrolactone by high performance capillary zone electrophories[J]. Chinese Journal of Analysis Laboratory,2001,20(1):91-92.
[17] 程劼,谢建春,苏晓鸥. 饲料中D-泛酸钙的超临界流体色谱测定[J].分析测试学报,2010,29(4):418-420.

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液相色谱-单四级杆法检测奶粉中泛酸的应用

Application of Pantothenic Acid in Milk Powder by Liquid Chromatography-single Quadrupole Method

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