中国乳业 ›› 2025, Vol. 0 ›› Issue (4): 84-88.doi: 10.12377/1671-4393.25.04.15

• 质量安全 • 上一篇    下一篇

提取剂对酶联免疫法检测芝士粉中黄曲霉毒素M1的影响

伍蓝, 黄华, 戴晓红, 方娟, 马后扬, 王丹, 吴建勋   

  1. 蒙牛乳制品武汉有限责任公司,湖北武汉 430040
  • 发布日期:2025-05-08
  • 作者简介:伍 蓝（1990-）,女,湖北安陆人,本科,研究方向为食品分析与检测;黄 华（1978-）,男,内蒙古呼和浩特人,硕士,研究方向为企业管理;戴晓红（1987-）,女,湖北黄冈人,本科,研究方向为食品安全;方 娟（1981-）,女,湖北武汉人,本科,研究方向为企业管理;马后扬（1986-）,男,湖北武汉人,本科,研究方向为企业管理;王 丹（1983-）,女,内蒙古呼和浩特人,硕士,副高级工程师,研究方向为企业管理;吴建勋（1981-）,男,内蒙古乌兰浩特人,本科,研究方向为企业管理。

Effect of Extracts on Determination of Aflatoxin M1 in Cheese Powde by ELISA

WU Lan, HUANG Hua, DAI Xiaohong, FANG Juan, MA Houyang, WANG Dan, WU Jianxun   

  1. Mengniu Dairy Products Wuhan Co.,Ltd.,Wuhan Hubei 430040
  • Published:2025-05-08

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摘要： [目的]本研究使用酶联免疫法（ELISA法）评估了一级水、无水甲醇、二氯甲烷3 种不同提取剂在检测芝士粉中黄曲霉毒素M1含量方面的效果。[方法]通过对比一级水、无水甲醇、二氯甲烷3 种不同提取剂提取效果的相对标准偏差,比较平均回收率。[结果]用一级水提取时,相对标准偏差2.56%,平均回收率35.30%~51.12%;用无水甲醇提取时,相对标准偏差4.20%,平均回收率81.32%~90.18%;用二氯甲烷提取时,样品组织状态异常,氮流蒸发后残留物呈油状,无法进行后续检测。[结论]以无水甲醇作为提取剂时,ELISA法的提取准确性较高,更适用于食品检测领域。

关键词: 酶联免疫法, 芝士粉, 黄曲霉毒素M1, 提取剂

Abstract: [Objective] Enzyme-linked immunoassay（ELISA）was used to evaluate the effects of three different extractants of primary water,anhydrous methanol and dichloromethane on the determination of aflatoxin M1 in cheese powder. [Method] The average recovery rate was compared by comparing the relative standard deviation of extraction effects of primary water,anhydrous methanol and dichloromethane. [Result] When extracted with primary water,the relative standard deviation was 2.56% and the average recovery was 35.30%~51.12%. When extracted with anhydrous methanol,the relative standard deviation was 4.20%,and the average recovery was 81.32%~90.18%. When extracted with dichloromethane,the tissue status of the sample was abnormal,and the residue after nitrogen evaporation was oily,which could not be further determinated. [Conclusion] When extracted with anhydrous methanol. ELISA method had higher extraction accuracy and is more suitable for food determination.

Key words: ELISA, cheese powder, Aflatoxin M1, extractant

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