婴幼儿配方奶粉中甘油三酯Sn-2位脂肪酸的检测

中国乳业 ›› 2019, Vol. 0 ›› Issue (7): 67-70.

婴幼儿配方奶粉中甘油三酯Sn-2位脂肪酸的检测

王俊芳¹, 杨国良², 王荣艳^3,*

1 杭州千岛湖天鑫有限公司滨江分公司;
2 杭州璞湃科技有限公司;
3 杭州立高检验检测技术研究院有限公司

出版日期:2019-07-25 发布日期:2021-03-19
通讯作者: *王荣艳（1982-）,女,硕士,中级工程师,现工作于杭州立高检验检测技术研究院有限公司,研究方向为食品营养与安全检测与研究。
作者简介:王俊芳,助理工程师,研究方向为食品与化妆品的仪器分析技术。

Online:2019-07-25 Published:2021-03-19

摘要/Abstract

摘要： 建立一种气相色谱测定婴幼儿配方奶粉中甘油三酯Sn-2位脂肪酸的方法。采用乙醚石油醚方法提取奶粉中的脂肪,猪胰脂酶水解甘油三酯中的1,3位脂肪酸,得到Sn-2单甘酯,通过氨基固相萃取柱分离出Sn-2单甘酯,甲酯化衍生后,再用气相色谱-氢火焰离子化检测器测定。加标回收率在81.85%~88.9%,相对标准偏差为2.13%,回收率和重现性满足检测要求。用NH₂固相萃取柱法（SPE）代替传统的薄层色谱法（TLC）分离单甘酯,操作简单,结果准确,重现性好,适用于婴幼儿配方奶粉中Sn-2位脂肪酸。

关键词: 婴幼儿配方奶粉, Sn-2位脂肪酸, 胰脂肪酶, 固相萃取, 气相色谱

王俊芳, 杨国良, 王荣艳. 婴幼儿配方奶粉中甘油三酯Sn-2位脂肪酸的检测[J]. 中国乳业, 2019, 0(7): 67-70.

参考文献

[1] Small D M.The effects of glyceride structure on absorption and metabolism[J]. Annual Review of Nutrition,1991,11(1):413-434.
[2] Jensen C,Buist N R M,Wilson T. Absorption of individual fatty acids from long chain or medium chain triglycerides in very small infants[J]. The American Journal of Clinical Nutrition,1986,43(5):745-751.
[3] Lopez- Lopez A,Castellote-Bargallo A I,Campoy-Folgoso C,et al. The influence of dietary palmitic acid triacylglyceride position on the fatty acid,calcium and magnesium contents of at term newborn faeces[J]. Early Human Development,2001,65(Suppl):S83-S94.
[4] Carnielli V P,Luijendijk I H T,Van Goudoever J B,et al. Structural position and amount of palmitic acid in infant formulas:effects on fat,fatty acid,and mineral balance[J]. Journal of Pediatric Gastroenterology and Nutrition,1996,23(5):553-560.
[5] Carnielli V P,Luijendijk I H,Van Goudoever J B,et al. Feeding premature newborn infants palmitic acid in amounts and stereoisomeric position similar to that of human milk:effects on fat and mineral balance[J]. American Journal of Clinical Nutrition,1995,61(5):1037-1042.
[6] Nelson S E, Frantz J A,Ziegler E E.Absorption of fat and calcium by infants fed a milk-based formula containing palm olein[J]. Journal of the American College of Nutrition,1998 , 17(4):327-332.
[7] Brecknridge W C,Marai L,Kuksis A.Triglyceride structure of human milk fat[J]. Canadian Journal of Biochemistry,1969,47(8):761-769.
[8] Matin J C,P Bougnoux P,Antoine J M,et al. Triayclglycerol structure of human colostrum and mature milk.[J]. Lipids,1993,28(7):637-643.
[9] Bracco U.Effect of triglyceride structure on fat absorption[J]. American Journal of Clinical Nutrition,1994,60(6 Suppl):1002s-1009s.
[10] Brockerhoff H,Yurkowski M J.Stereospecific analyses of several vegetable fats[J]. Journal of Lipid Research. 1966,7(1):62-64.
[11] 韩瑞丽,马健,张佳程,等. 牛乳脂肪甘油三酯中sn-2位脂肪酸组成的分析[J]. 乳业科学与技术,2009,32(2):71-73.
[12] 贾云虹,李朝旭,房新平,等. 婴儿配方乳粉中甘油三脂Sn-2位脂肪酸的测定[J].食品研究与开发, 2014,35(2):87-91.
[13] 国家标准总局. GB 5009.6—2016 [S]. 北京:中国标准出版社,2016.
[14] 刘彪,叶文慧,郭顺堂. 母乳及市售婴儿配方奶粉中脂肪酸结构分析[J]. 中国食品学报,2018,18(10):246-251.

Metrics

Viewed

Full text

Abstract

Cited

Shared

Discussed