中国乳业 ›› 2026, Vol. 0 ›› Issue (8): 89-96.doi: 10.12377/1671-4393.26.08.13
易明, 陈娜, 赵新宇, 邢宇*
YI Ming, CHEN Na, ZHAO Xinyu, XING Yu*
摘要: 益生菌的应用受限于食品加工、储存过程及胃肠道环境所导致的活性损耗。包埋技术是应对上述挑战的关键策略。本文重点综述了近年来益生菌包埋技术领域的最新研究进展。当前,包埋材料以多糖和蛋白质为主,并以脂质为辅,构成传统主流趋势;与此同时,新型植物提取物和多酚类物质正迅速成为研究热点。包埋方法除传统喷雾干燥、冷冻干燥、乳化法和挤出法外,新兴技术如3D打印和微流控等已获得广泛关注。此外,本文介绍了益生菌包埋技术在食品领域的应用现状,并基于此对其未来发展前景进行了展望。
| [1] Hill C,Guarner F,Reid G,et al. The international scientific association for probiotics and prebiotics consensus statement on the scope and appropriate use of the term probiotic[J]. Nature Reviews Gastroenterology & Hepatology,2014,11(8):506-514. [2] Yuan Y,Yin M,Zhai Q,et al. The encapsulation strategy to improve the survival of probiotics for food application:From rough multicellular to single-cell surface engineering and microbial mediation[J]. Critical Reviews in Food Science and Nutrition,2024,64(10):2794-2810. [3] Kim N,Yun M,Oh Y J,et al. Mind-altering with the gut:Modulation of the gut-brain axis with probiotics[J]. Journal of Microbiology,2018,56(3):172-182. [4] Knezevic J,Starchl C,Berisha A T,et al. Thyroid-gut-axis:How does the microbiota influence thyroid function?[J]. Nutrients,2020,12(6):1769. [5] López-Moreno A,Aguilera M. Probiotics dietary supplementation for modulating endocrine and fertility microbiota dysbiosis[J]. Nutrients,2020,12(3):757. [6] Hu S,Wang L,Jiang Z. Dietary additive probiotics modulation of the intestinal microbiota[J]. Protein and Peptide Letters,2017,24(5):382-387. [7] Huang L,Thonusin C,Chattipakorn N,et al. Impacts of gut microbiota on gestational diabetes mellitus:A comprehensive review[J]. European Journal of Nutrition,2021,60(5):2343-2360. [8] Yun S I,Park H O,Kang J H. Effect of Lactobacillus gasseri BNR17 on blood glucose levels and body weight in a mouse model of type 2 diabetes[J]. Journal of Applied Microbiology,2009,107(5):1681-1686. [9] Sanchis-Chordà J,Del Pulgar E M G,Carrasco-Luna J,et al. Bifidobacterium pseudocatenulatum CECT 7765 supplementation improves inflammatory status in insulin-resistant obese children[J]. European Journal of Nutrition,2019,58(7):2789-2800. [10] Fedorak R N,Feagan B G,Hotte N,et al. The probiotic VSL#3 has anti-inflammatory effects and could reduce endoscopic recurrence after surgery for Crohn's disease[J]. Clinical Gastroenterology and Hepatology,2015,13(5):928-935. [11] Jang S E,Jeong J J,Kim J K,et al. Simultaneous amelioratation of colitis and liver injury in mice by Bifidobacterium longum LC67 and Lactobacillus plantarum LC27[J]. Scientific Reports,2018,8(1):7500. [12] Kato S,Hamouda N,Kano Y,et al. Probiotic Bifidobacterium bifidum G9-1 attenuates 5-fluorouracil-induced intestinal mucositis in mice via suppression of dysbiosis-related secondary inflammatory responses[J]. Clinical & Experimental Pharmacology & Physiology,2017,44(10):1017-1025. [13] Aura I,Sigita P. Encapsulation of probiotics:Proper selection of the probiotic strain and the influence of encapsulation technology and materials on the viability of encapsulated microorganisms[J]. Probiotics and Antimicrobial Proteins,2018,10(1):1-10. [14] 陈睿,康旭,袁江兰,等.猪血浆蛋白-海藻酸钠凝胶珠对植物乳杆菌的包埋效果研究[J].食品与发酵工业,2025,51(6):218-223. [15] Kuo C C,Clark S,Qin H,et al.Development of a shelf-stable,gel-based delivery system for probiotics by encapsulation,3D printing,and freeze-drying[J].LWT-Food Science and Technology,2022(157):113075. [16] Zubair W,Afzaal M,Saeed F,et al. Survivability of Lactobacillus rhamnosus under stressed conditions as affected by taro starch(Colocasia esculenta) encapsulation[J]. International Journal of Food Properties,2023,26(2):3465-3476. [17] Afzaal M,Saeed F,Ateeq H,et al. Encapsulation of Bifidobacterium bifidum by internal gelation method to access the viability in cheddar cheese and under simulated gastrointestinal conditions[J]. Food Science & Nutrition,2020,8(6):2739-2747. [18] 陈良晓.西瓜皮果胶复合包埋嗜酸乳杆菌的研究[D].泰安:山东农业大学,2024. [19] Afzaal M,Saeed F,Ateeq H,et al. Effect of cellulose-chitosan hybrid-based encapsulation on the viability and stability of probiotics under simulated gastric transit and in kefir[J]. Biomimetics,2022,7(3):109. [20] 马乐.基于乳清蛋白-甲壳素纤维复合凝聚物构建益生菌微胶囊及其应用研究[D]广州:广州大学,2024. [21] Liu J,Liu F,Ren T,et al. Fabrication of fish gelatin/sodium alginate double network gels for encapsulation of probiotics[J]. Journal of the Science of Food and Agriculture,2021,101(10):4398-4408. [22] 高向新.双歧杆菌微胶囊的工艺优化及性能评价[D].呼和浩特:内蒙古农业大学,2023. [23] 张芸.益生菌负载油包水乳液的构建及其相关产品开发[D].南昌:南昌大学,2022. [24] 姜兆炜.基于益生菌和鱼油的W/O/W乳液改善溃疡性结肠炎的功效及其在酸奶中的应用[D].咸阳:西北农林科技大学,2023. [25] Vimon S,Romyasamit C,Chanpakdee R,et al. Eco-friendly microencapsulation of Lacticaseibacillus Paracasei using cissampelos pareira leaf extract as natural encapsulating materials[J].Preprints,2024. [26] Centurion F,Merhebi S,Baharfar M,et al. Cell-mediated biointerfacial phenolic assembly for probiotic nano encapsulation[J]. Advanced Functional Materials,2022,32(26):2200775. [27] Frakolaki G,Giannou V,Kekos D,et al. A review of the microencapsulation techniques for the incorporation of probiotic bacteria in functional foods[J]. Critical Reviews in Food Science and Nutrition,2021,61(9):1515-1536. [28] 李思媛,孙晓琛,端木传宇,等.含益生菌的微胶囊制备及稳定性研究[J].中国食品学报,2024,24(10):238-245. [29] Solmaz B J,Christos S,Lina Y,et al. Optimization of spray-drying process conditions for the production of maximally viable microencapsulated L. acidophilus NCIMB 701748[J]. Drying Technology,2013,31(11):1274-1283. [30] Betoret E,Betoret N,Barrera C,et al. Effect of drying process,encapsulation,and storage on the survival rates and gastrointestinal resistance of L. salivarius spp. salivarius included into a fruit matrix[J]. Microorganisms,2020,8(5):654. [31] Singh P,Medronho B,Miguel M G,et al. On the encapsulation and viability of probiotic bacteria in edible carboxymethyl cellulose-gelatin water-in-water emulsions[J]. Food Hydrocolloids,2018(75):41-50. [32] Shahrubudin N,Lee T C,Ramlan R. An overview on 3D printing technology:Technological,materials,and applications[J]. Procedia Manufacturing,2019(35):1286-1296. [33] 端木传宇.基于蛋白/多糖油水双凝胶的益生菌包埋及其在3D打印食品中的应用研究[D].镇江:江苏大学,2024. [34] Velasco D,Tumarkin E,Kumacheva E. Microfluidic encapsulation of cells in polymer microgels[J]. Small,2012,8(11):1633-1642. [35] Lee K G,Park T J,Soo S Y,et al. Synthesis and utilization of E. coli-encapsulated PEG-based microdroplet using a microfluidic chip for biological application[J]. Biotechnology and Bioengineering,2010,107(4):747-751. [36] 谢鑫.微流控制备多壳层胶体囊用于肠道益生菌递送研究[D].成都:西南交通大学,2023. [37] Pour H M,Marhamatizadeh M H,Fattahi H. Encapsulation of different types of probiotic bacteria within conventional/multilayer emulsion and its effect on the properties of probiotic yogurt[J]. Journal of Food Quality,2022,2022(1):7923899. [38] Frakolaki G,Kekes T,Lympaki F,et al. Use of encapsulated Bifidobacterium animalis subsp. lactis through extrusion or emulsification for the production of probiotic yogurt[J]. Journal of Food Process Engineering,2021,45(7):e13792. [39] Mukhtar H,Yaqub S,Haq I U. Production of probiotic Mozzarella cheese by incorporating locally isolated Lactobacillus acidophilus[J]. Annals of Microbiology,2020,70(1):240-251. [40] Ortakci F,Broadbent J R,McManus W R,et al. Survival of microencapsulated probiotic Lactobacillus paracasei LBC-1e during manufacture of Mozzarella cheese and simulated gastric digestion[J]. Journal of Dairy Science,2012,95(11):6274-6281. [41] Hossain M N,Ranadheera C S,Fang Z,et al. Impact of encapsulating probiotics with cocoa powder on the viability of probiotics during chocolate processing,storage,and in vitro gastrointestinal digestion[J]. Journal of Food Science,2021,86(5):1629-1641. [42] Hossain M N,Senaka R C,Ranadheera C S,Fang Z,et al. Production of short chain fatty acids and vitamin B12 during the in-vitro digestion and fermentation of probiotic chocolate[J]. Food Bioscience,2022(47):101682. [43] Ding W ,Shah N . Survival of free and microencapsulated probiotic bacteria in orange and apple juices[J]. International Food Research Journal,2008,15(2):219-232. [44] Afzaal M,Saeed F,Saeed M,et al. Survival and stability of free and encapsulated probiotic bacteria under simulated gastrointestinal conditions and in pasteurized grape juice[J]. Journal of Food Processing and Preservation,2020,44(3):e14346. [45] Hassan S S,Ahmad Fadzil I N,Yusoff A,et al. A review on microencapsulation in improving probiotic stability for beverages application[J]. Science Letters(ScL),2020,14(1):49-61. [46] Afzaal M,Saeed F,Arshad M U,et al.The effect of encapsulation on the stability of probiotic bacteria in ice cream and simulated gastrointestinal conditions[J]. Probiotics and Antimicrobial Proteins,2019,11(4):1348-1354. [47] Kent R M,Doherty S B. Probiotic bacteria in infant formula and follow-up formula:Microencapsulation using milk and pea proteins to improve microbiological quality[J]. Food Research International,2014(64):567-576. [48] Weinbreck F,Bodnár I,Marco M L. Can encapsulation lengthen the shelf-life of probiotic bacteria in dry products?[J]. International Journal of Food Microbiology,2010,136(3):364-367. |
| [1] | 王礞礞, 张超, 田园, 王晶. 反刍动物饲养中抗生素替代型添加剂的现状与应用前景[J]. 中国乳业, 2026, 0(7): 33-39. |
| [2] | 刘照雷. 益生菌制剂在降低奶牛乳房炎发生率中的应用效果评价[J]. 中国乳业, 2026, 0(6): 111-116. |
| [3] | 李继庆. 益生菌发酵饲料对热应激奶牛肠道菌群、血清应激因子及产奶性能的影响[J]. 中国乳业, 2026, 0(2): 23-28. |
| [4] | 徐英霞. 复合益生菌制剂对奶牛生产性能、瘤胃发酵和血清指标的影响[J]. 中国乳业, 2025, 0(7): 60-65. |
| [5] | 张兵, 付强, 唐世云, 孟卫芹, 付石军. 益生菌在功能性乳制品中功效与作用机制研究进展[J]. 中国乳业, 2025, 0(5): 93-99. |
| [6] | 刘敦章, 胡定党. 益生菌发酵饲料对真胃炎奶牛肠道菌群和生产性能的影响[J]. 中国乳业, 2025, 0(3): 57-60. |
| [7] | 张尊会, 郭玲玲. 乳源免疫球蛋白的功能特性及其在食品工业中的应用研究[J]. 中国乳业, 2025, 0(12): 85-92. |
| [8] | 李凯锋, 胡姝敏, 宫春颖, 王帅, 王青云. 牛初乳益生菌粉调节免疫和肠道菌群保健功能的研究[J]. 中国乳业, 2024, 0(9): 73-79. |
| [9] | 王国骄, 洪青, 刘振民, 王吉栋, 雍靖怡. 益生菌发酵乳对机体健康作用的研究进展[J]. 中国乳业, 2024, 0(7): 106-110. |
| [10] | 陈春雷. 益生菌发酵饲料对奶牛肠道菌群和生产性能的影响[J]. 中国乳业, 2024, 0(5): 53-57. |
| [11] | 蔡云阁. 益生菌治疗奶牛乳腺炎的研究进展[J]. 中国乳业, 2023, 0(4): 67-71. |
| [12] | 银佳, 冯立科, 杨爱君, 彭小霞, 李理. 产B族维生素微生物研究进展[J]. 中国乳业, 2023, 0(10): 105-115. |
| [13] | 高雁, 杨胜春, 曾军, 霍向东, 孙建, 林青. 益生菌对断奶后犊牛生长性能及血清指标的影响[J]. 中国乳业, 2022, 0(8): 19-23. |
| [14] | 杨露. 益生菌对奶牛产奶量、乳成分及瘤胃微生物区系的影响[J]. 中国乳业, 2022, 0(8): 15-18. |
| [15] | 杨鹏飞, 吴桐, 谢奎, 邓友田, 蒋飞荣, 卢素云, 侯艳梅. 配方羊奶粉对小鼠免疫功能影响的研究[J]. 中国乳业, 2021, 0(12): 133-136. |
|
||