奶粉中胆固醇的测定方法验证

doi:10.12377/1671-4393.22.08.15

中国乳业 ›› 2022, Vol. 0 ›› Issue (8): 65-69.doi: 10.12377/1671-4393.22.08.15

奶粉中胆固醇的测定方法验证

崔承远

北大荒完达山乳业股份有限公司,黑龙江哈尔滨 150078

出版日期:2022-08-25 发布日期:2022-09-02
作者简介:崔承远（1986-）,男,黑龙江哈尔滨人,硕士,食品检测工程师,研究方向为婴幼儿配方奶粉及成人奶粉检测。

Verification of Determination Method of Cholesterol in Milk Powder

CUI Chengyuan

The Great Northern Wilderness Wandashan Dairy Co.,Ltd.,Harbin Heilongjiang 150078

Online:2022-08-25 Published:2022-09-02

摘要/Abstract

摘要： 为了提高日常奶粉中胆固醇的检测效率,本文建立了一种更为便捷的气相色谱法检测奶粉中的胆固醇。通过乙醇和氢氧化钾溶液对奶粉试样进行皂化反应,再以石油醚对样液中的胆固醇组分进行提取,最后通过平行浓缩仪将提取液浓缩至近干,再以正己烷定容,采用气相色谱分析法,外标法定量。本方法具有检出限低、精密度高、正确度高、操作过程简便、效率更高简便等优点。

关键词: 胆固醇, 气相色谱法, 皂化反应, 浓缩, 外标法

Abstract: In order to improve the detection efficiency of cholesterol in daily milk powder,a more convenient gas chromatography method was established in this paper.The sample of milk powder was saponified by ethanol and potassium hydroxide solution,and then the cholesterol component in the sample solution was extracted by petroleum ether. Finally,the extract was concentrated to near dryness by a parallel concentrator,then the volume was fixed by n-hexane,and the content was quantified by gas chromatography and external standard method.This method has the advantages of low detection limit,high precision,high accuracy,simple operation process,higher efficiency and simplicity.

Key words: cholesterol, gas chromatography, saponification, concentration, external standard method

中图分类号:

TS207.3

崔承远. 奶粉中胆固醇的测定方法验证[J]. 中国乳业, 2022, 0(8): 65-69.

CUI Chengyuan. Verification of Determination Method of Cholesterol in Milk Powder[J]. China Dairy, 2022, 0(8): 65-69.

参考文献

[1] 郭玮,陈方俊,潘柏申.胆固醇检测方法的发展历史及标准化[J].检验医学,2013,28(11):970-974.
[2] 王启辉,刘洋,张经华.生物体内固醇类物质检测方法的研究进展[J].现代科学仪器,2010,2:176-179,183.
[3] 张晓儒. 内蒙古家畜胆固醇含量测定及两种检测方法比较:硕士论文[D].呼和浩特:内蒙古农业大学,2017.
[4] 朱炳祺,刘柱,陈万勤.食品中胆固醇的检测方法的实验室转移确认验证[R].中国药学会第二届药物检测质量管理学术研讨会资料汇编,2015.
[5] 张庆波. 气相色谱质谱法检测食品中胆固醇含量的研究:硕士论文[D].长春:吉林大学,2013.
[6] 徐志红,王金宇,李淑芬.气相色谱法测定鹿茸萃取物中胆固醇的含量[J].食品科学,2007,6(28):269-271.
[7] 龚蕊,苗虹,金绍明.气相色谱-质谱法测定食用油中氯丙醇酯的含量方法验证研究[J].中国食品卫生杂志,2016,3:333-339.
[8] 朱燕萍. 气相色谱法测定食品中乙酰丙酸的方法验证[J].食品界,2018,6:94-98.

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奶粉中胆固醇的测定方法验证

Verification of Determination Method of Cholesterol in Milk Powder

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