中国乳业 ›› 2026, Vol. 0 ›› Issue (8): 89-96.doi: 10.12377/1671-4393.26.08.13

• 乳品加工 • 上一篇    下一篇

包埋技术在提升益生菌食品加工与储存稳定性中的研究进展

易明, 陈娜, 赵新宇, 邢宇*   

  1. 辽宁越秀辉山控股股份有限公司,辽宁沈阳 110000
  • 发布日期:2026-09-17
  • 通讯作者: *邢 宇(1981-),男,辽宁沈阳人,硕士,高级工程师,研究方向为乳制品加工与研发。
  • 作者简介:易 明(1995-),男,辽宁鞍山人,硕士,工程师,研究方向为乳制品加工与研发;陈 娜(1977-),女,天津人,硕士,正高级工程师,研究方向为乳制品加工与研发;赵新宇(1985-),男,辽宁本溪人,硕士,工程师,研究方向为乳制品加工与研发。

Research Progress on Encapsulation Technology in Enhancing the Stability of Probiotics during Food Processing and Storage

YI Ming, CHEN Na, ZHAO Xinyu, XING Yu*   

  1. Liaoning Yuexiu Huishan Holdings Co.,Ltd.,Shenyang Liaoning 110000
  • Published:2026-09-17

摘要: 益生菌的应用受限于食品加工、储存过程及胃肠道环境所导致的活性损耗。包埋技术是应对上述挑战的关键策略。本文重点综述了近年来益生菌包埋技术领域的最新研究进展。当前,包埋材料以多糖和蛋白质为主,并以脂质为辅,构成传统主流趋势;与此同时,新型植物提取物和多酚类物质正迅速成为研究热点。包埋方法除传统喷雾干燥、冷冻干燥、乳化法和挤出法外,新兴技术如3D打印和微流控等已获得广泛关注。此外,本文介绍了益生菌包埋技术在食品领域的应用现状,并基于此对其未来发展前景进行了展望。

关键词: 益生菌, 包埋技术, 存活率, 包埋壁材, 3D打印, 微流控, 功能性食品

Abstract: The application of probiotics is constrained by activity loss resulting from food processing,storage processes,and gastrointestinal environment. Embedding technology serves as a key strategy to address these challenges. This paper mainly reviewed the latest advances in the field of probiotic embedding technology in recent years. Currently,the encapsulation materials primarily consist of polysaccharides and proteins,with lipids serving as a secondary component,forming the traditional mainstream trend;meanwhile,novel plant extracts and polyphenols are rapidly rapidly becoming research hotspots. Beyond conventional methods such as spray drying,freeze drying,emulsification and extrusion,emerging technologies such as 3D printing and microfluidics have also received considerable attention. Furthermore,this paper presented the current application status of probiotic embedding technology in the food industry,and based on this,it also forecasted its future development prospects.

Key words: probiotics, encapsulation technology, survival rate, encapsulation wall material, 3D printing, microfluidics, functional food

[1] Hill C,Guarner F,Reid G,et al. The international scientific association for probiotics and prebiotics consensus statement on the scope and appropriate use of the term probiotic[J]. Nature Reviews Gastroenterology & Hepatology,2014,11(8):506-514.
[2] Yuan Y,Yin M,Zhai Q,et al. The encapsulation strategy to improve the survival of probiotics for food application:From rough multicellular to single-cell surface engineering and microbial mediation[J]. Critical Reviews in Food Science and Nutrition,2024,64(10):2794-2810.
[3] Kim N,Yun M,Oh Y J,et al. Mind-altering with the gut:Modulation of the gut-brain axis with probiotics[J]. Journal of Microbiology,2018,56(3):172-182.
[4] Knezevic J,Starchl C,Berisha A T,et al. Thyroid-gut-axis:How does the microbiota influence thyroid function?[J]. Nutrients,2020,12(6):1769.
[5] López-Moreno A,Aguilera M. Probiotics dietary supplementation for modulating endocrine and fertility microbiota dysbiosis[J]. Nutrients,2020,12(3):757.
[6] Hu S,Wang L,Jiang Z. Dietary additive probiotics modulation of the intestinal microbiota[J]. Protein and Peptide Letters,2017,24(5):382-387.
[7] Huang L,Thonusin C,Chattipakorn N,et al. Impacts of gut microbiota on gestational diabetes mellitus:A comprehensive review[J]. European Journal of Nutrition,2021,60(5):2343-2360.
[8] Yun S I,Park H O,Kang J H. Effect of Lactobacillus gasseri BNR17 on blood glucose levels and body weight in a mouse model of type 2 diabetes[J]. Journal of Applied Microbiology,2009,107(5):1681-1686.
[9] Sanchis-Chordà J,Del Pulgar E M G,Carrasco-Luna J,et al. Bifidobacterium pseudocatenulatum CECT 7765 supplementation improves inflammatory status in insulin-resistant obese children[J]. European Journal of Nutrition,2019,58(7):2789-2800.
[10] Fedorak R N,Feagan B G,Hotte N,et al. The probiotic VSL#3 has anti-inflammatory effects and could reduce endoscopic recurrence after surgery for Crohn's disease[J]. Clinical Gastroenterology and Hepatology,2015,13(5):928-935.
[11] Jang S E,Jeong J J,Kim J K,et al. Simultaneous amelioratation of colitis and liver injury in mice by Bifidobacterium longum LC67 and Lactobacillus plantarum LC27[J]. Scientific Reports,2018,8(1):7500.
[12] Kato S,Hamouda N,Kano Y,et al. Probiotic Bifidobacterium bifidum G9-1 attenuates 5-fluorouracil-induced intestinal mucositis in mice via suppression of dysbiosis-related secondary inflammatory responses[J]. Clinical & Experimental Pharmacology & Physiology,2017,44(10):1017-1025.
[13] Aura I,Sigita P. Encapsulation of probiotics:Proper selection of the probiotic strain and the influence of encapsulation technology and materials on the viability of encapsulated microorganisms[J]. Probiotics and Antimicrobial Proteins,2018,10(1):1-10.
[14] 陈睿,康旭,袁江兰,等.猪血浆蛋白-海藻酸钠凝胶珠对植物乳杆菌的包埋效果研究[J].食品与发酵工业,2025,51(6):218-223.
[15] Kuo C C,Clark S,Qin H,et al.Development of a shelf-stable,gel-based delivery system for probiotics by encapsulation,3D printing,and freeze-drying[J].LWT-Food Science and Technology,2022(157):113075.
[16] Zubair W,Afzaal M,Saeed F,et al. Survivability of Lactobacillus rhamnosus under stressed conditions as affected by taro starch(Colocasia esculenta) encapsulation[J]. International Journal of Food Properties,2023,26(2):3465-3476.
[17] Afzaal M,Saeed F,Ateeq H,et al. Encapsulation of Bifidobacterium bifidum by internal gelation method to access the viability in cheddar cheese and under simulated gastrointestinal conditions[J]. Food Science & Nutrition,2020,8(6):2739-2747.
[18] 陈良晓.西瓜皮果胶复合包埋嗜酸乳杆菌的研究[D].泰安:山东农业大学,2024.
[19] Afzaal M,Saeed F,Ateeq H,et al. Effect of cellulose-chitosan hybrid-based encapsulation on the viability and stability of probiotics under simulated gastric transit and in kefir[J]. Biomimetics,2022,7(3):109.
[20] 马乐.基于乳清蛋白-甲壳素纤维复合凝聚物构建益生菌微胶囊及其应用研究[D]广州:广州大学,2024.
[21] Liu J,Liu F,Ren T,et al. Fabrication of fish gelatin/sodium alginate double network gels for encapsulation of probiotics[J]. Journal of the Science of Food and Agriculture,2021,101(10):4398-4408.
[22] 高向新.双歧杆菌微胶囊的工艺优化及性能评价[D].呼和浩特:内蒙古农业大学,2023.
[23] 张芸.益生菌负载油包水乳液的构建及其相关产品开发[D].南昌:南昌大学,2022.
[24] 姜兆炜.基于益生菌和鱼油的W/O/W乳液改善溃疡性结肠炎的功效及其在酸奶中的应用[D].咸阳:西北农林科技大学,2023.
[25] Vimon S,Romyasamit C,Chanpakdee R,et al. Eco-friendly microencapsulation of Lacticaseibacillus Paracasei using cissampelos pareira leaf extract as natural encapsulating materials[J].Preprints,2024.
[26] Centurion F,Merhebi S,Baharfar M,et al. Cell-mediated biointerfacial phenolic assembly for probiotic nano encapsulation[J]. Advanced Functional Materials,2022,32(26):2200775.
[27] Frakolaki G,Giannou V,Kekos D,et al. A review of the microencapsulation techniques for the incorporation of probiotic bacteria in functional foods[J]. Critical Reviews in Food Science and Nutrition,2021,61(9):1515-1536.
[28] 李思媛,孙晓琛,端木传宇,等.含益生菌的微胶囊制备及稳定性研究[J].中国食品学报,2024,24(10):238-245.
[29] Solmaz B J,Christos S,Lina Y,et al. Optimization of spray-drying process conditions for the production of maximally viable microencapsulated L. acidophilus NCIMB 701748[J]. Drying Technology,2013,31(11):1274-1283.
[30] Betoret E,Betoret N,Barrera C,et al. Effect of drying process,encapsulation,and storage on the survival rates and gastrointestinal resistance of L. salivarius spp. salivarius included into a fruit matrix[J]. Microorganisms,2020,8(5):654.
[31] Singh P,Medronho B,Miguel M G,et al. On the encapsulation and viability of probiotic bacteria in edible carboxymethyl cellulose-gelatin water-in-water emulsions[J]. Food Hydrocolloids,2018(75):41-50.
[32] Shahrubudin N,Lee T C,Ramlan R. An overview on 3D printing technology:Technological,materials,and applications[J]. Procedia Manufacturing,2019(35):1286-1296.
[33] 端木传宇.基于蛋白/多糖油水双凝胶的益生菌包埋及其在3D打印食品中的应用研究[D].镇江:江苏大学,2024.
[34] Velasco D,Tumarkin E,Kumacheva E. Microfluidic encapsulation of cells in polymer microgels[J]. Small,2012,8(11):1633-1642.
[35] Lee K G,Park T J,Soo S Y,et al. Synthesis and utilization of E. coli-encapsulated PEG-based microdroplet using a microfluidic chip for biological application[J]. Biotechnology and Bioengineering,2010,107(4):747-751.
[36] 谢鑫.微流控制备多壳层胶体囊用于肠道益生菌递送研究[D].成都:西南交通大学,2023.
[37] Pour H M,Marhamatizadeh M H,Fattahi H. Encapsulation of different types of probiotic bacteria within conventional/multilayer emulsion and its effect on the properties of probiotic yogurt[J]. Journal of Food Quality,2022,2022(1):7923899.
[38] Frakolaki G,Kekes T,Lympaki F,et al. Use of encapsulated Bifidobacterium animalis subsp. lactis through extrusion or emulsification for the production of probiotic yogurt[J]. Journal of Food Process Engineering,2021,45(7):e13792.
[39] Mukhtar H,Yaqub S,Haq I U. Production of probiotic Mozzarella cheese by incorporating locally isolated Lactobacillus acidophilus[J]. Annals of Microbiology,2020,70(1):240-251.
[40] Ortakci F,Broadbent J R,McManus W R,et al. Survival of microencapsulated probiotic Lactobacillus paracasei LBC-1e during manufacture of Mozzarella cheese and simulated gastric digestion[J]. Journal of Dairy Science,2012,95(11):6274-6281.
[41] Hossain M N,Ranadheera C S,Fang Z,et al. Impact of encapsulating probiotics with cocoa powder on the viability of probiotics during chocolate processing,storage,and in vitro gastrointestinal digestion[J]. Journal of Food Science,2021,86(5):1629-1641.
[42] Hossain M N,Senaka R C,Ranadheera C S,Fang Z,et al. Production of short chain fatty acids and vitamin B12 during the in-vitro digestion and fermentation of probiotic chocolate[J]. Food Bioscience,2022(47):101682.
[43] Ding W ,Shah N . Survival of free and microencapsulated probiotic bacteria in orange and apple juices[J]. International Food Research Journal,2008,15(2):219-232.
[44] Afzaal M,Saeed F,Saeed M,et al. Survival and stability of free and encapsulated probiotic bacteria under simulated gastrointestinal conditions and in pasteurized grape juice[J]. Journal of Food Processing and Preservation,2020,44(3):e14346.
[45] Hassan S S,Ahmad Fadzil I N,Yusoff A,et al. A review on microencapsulation in improving probiotic stability for beverages application[J]. Science Letters(ScL),2020,14(1):49-61.
[46] Afzaal M,Saeed F,Arshad M U,et al.The effect of encapsulation on the stability of probiotic bacteria in ice cream and simulated gastrointestinal conditions[J]. Probiotics and Antimicrobial Proteins,2019,11(4):1348-1354.
[47] Kent R M,Doherty S B. Probiotic bacteria in infant formula and follow-up formula:Microencapsulation using milk and pea proteins to improve microbiological quality[J]. Food Research International,2014(64):567-576.
[48] Weinbreck F,Bodnár I,Marco M L. Can encapsulation lengthen the shelf-life of probiotic bacteria in dry products?[J]. International Journal of Food Microbiology,2010,136(3):364-367.
[1] 王礞礞, 张超, 田园, 王晶. 反刍动物饲养中抗生素替代型添加剂的现状与应用前景[J]. 中国乳业, 2026, 0(7): 33-39.
[2] 刘照雷. 益生菌制剂在降低奶牛乳房炎发生率中的应用效果评价[J]. 中国乳业, 2026, 0(6): 111-116.
[3] 李继庆. 益生菌发酵饲料对热应激奶牛肠道菌群、血清应激因子及产奶性能的影响[J]. 中国乳业, 2026, 0(2): 23-28.
[4] 徐英霞. 复合益生菌制剂对奶牛生产性能、瘤胃发酵和血清指标的影响[J]. 中国乳业, 2025, 0(7): 60-65.
[5] 张兵, 付强, 唐世云, 孟卫芹, 付石军. 益生菌在功能性乳制品中功效与作用机制研究进展[J]. 中国乳业, 2025, 0(5): 93-99.
[6] 刘敦章, 胡定党. 益生菌发酵饲料对真胃炎奶牛肠道菌群和生产性能的影响[J]. 中国乳业, 2025, 0(3): 57-60.
[7] 张尊会, 郭玲玲. 乳源免疫球蛋白的功能特性及其在食品工业中的应用研究[J]. 中国乳业, 2025, 0(12): 85-92.
[8] 李凯锋, 胡姝敏, 宫春颖, 王帅, 王青云. 牛初乳益生菌粉调节免疫和肠道菌群保健功能的研究[J]. 中国乳业, 2024, 0(9): 73-79.
[9] 王国骄, 洪青, 刘振民, 王吉栋, 雍靖怡. 益生菌发酵乳对机体健康作用的研究进展[J]. 中国乳业, 2024, 0(7): 106-110.
[10] 陈春雷. 益生菌发酵饲料对奶牛肠道菌群和生产性能的影响[J]. 中国乳业, 2024, 0(5): 53-57.
[11] 蔡云阁. 益生菌治疗奶牛乳腺炎的研究进展[J]. 中国乳业, 2023, 0(4): 67-71.
[12] 银佳, 冯立科, 杨爱君, 彭小霞, 李理. 产B族维生素微生物研究进展[J]. 中国乳业, 2023, 0(10): 105-115.
[13] 高雁, 杨胜春, 曾军, 霍向东, 孙建, 林青. 益生菌对断奶后犊牛生长性能及血清指标的影响[J]. 中国乳业, 2022, 0(8): 19-23.
[14] 杨露. 益生菌对奶牛产奶量、乳成分及瘤胃微生物区系的影响[J]. 中国乳业, 2022, 0(8): 15-18.
[15] 杨鹏飞, 吴桐, 谢奎, 邓友田, 蒋飞荣, 卢素云, 侯艳梅. 配方羊奶粉对小鼠免疫功能影响的研究[J]. 中国乳业, 2021, 0(12): 133-136.
Viewed
Full text


Abstract

Cited

  Shared   
  Discussed   
No Suggested Reading articles found!