关键词: 山羊, 大肠杆菌, 分离鉴定, 耐药性分析

Abstract: [Objective] The aim was to isolate and identify Escherichia coli from feces and tissues of sick goats in dairy goat farms in some areas of Linyi City,Shandong Province, and analyze its drug resistance. [Method] Sixty-eight samples of sick dairy goats were collected,including feces,blood,liver and spleen tissues,serous fibrous exudates in thoracic cavity and pericardial cavity,and sent to the laboratory for diagnosis. After pretreatment, Escherichia coli was successfully isolated by separation medium and biochemical identification method. [Results] In the drug sensitivity test,it was found that Escherichia coli showed high resistance to penicillin,streptomycin,azithromycin,clindamycin,gentamicin,levofloxacin and midecamycin,while it was sensitive to cefazolin,cefradine,ceftazidime and cefoperazone. The analysis of bacterial multidrug resistance showed that there were 9 kinds of antibiotics with drug resistance rate over 60%. [Conclusion] The drug resistance of Escherichia coli exists in dairy goat farms in some areas of Linyi City,Shandong Province. In order to ensure the development of local dairy goat breeding industry and the quality and safety of goat dairy products,corresponding prevention and control measures should be taken.

Key words: goat, Escherichia coli, isolation and identification, drug resistance analysis