中国乳业 ›› 2023, Vol. 0 ›› Issue (8): 99-104.doi: 10.12377/1671-4393.23.08.18

• 乳品加工 • 上一篇    下一篇

乳中酪蛋白生物活性多肽的潜在应用价值

邓应朝1, 高燕2   

  1. 1 重庆市秀山县龙凤坝镇农业服务中心,重庆 409900
    2 重庆市秀山县雅江镇人民政府,重庆 409900
  • 出版日期:2023-08-25 发布日期:2023-09-04
  • 作者简介:邓应朝(1971-),男,重庆人,大专,兽医师,研究方向为畜牧兽医;高 燕(1985-),女,土家族,重庆人,本科,畜牧师,研究方向为畜牧兽医。

Potential Applications of Casein Bioactive Peptides in Milk

DENG Yingchao1, GAO Yan2   

  1. 1Agricultural Service Center of Longfengba Town,Xiushan County,Chongqing 409900;
    2Chongqing People's Government of Yajiang Town,Xiushan County,Chongqing 409900
  • Online:2023-08-25 Published:2023-09-04

摘要: 酪蛋白作为乳中含量最高的蛋白质,具有较高营养价值。其主要由αs1-酪蛋白、αs2-酪蛋白、β-酪蛋白和κ-酪蛋白组成,四种构型的特点不同。酪蛋白经酶解或消化后能产生较多活性多肽,对调节氧化应激、降低血压、促进矿物质吸收、降低血糖和血脂、提高抗菌性和调节神经系统均有良好作用。活性多肽目前制备方法主要分为化学降解法、酶解法、微生物发酵法、基因工程法和化学合成法,各方法均有优缺点。为促进乳中酪蛋白的生物活性多肽开发,本文从酪蛋白组成特点、活性多肽类型和制备方法等方面综合概述,促进酪蛋白制备活性多肽工业化,增强乳的附加值。

关键词: 酪蛋白, 抗氧化, 降血压, 酶解法

Abstract: As the most abundant protein in milk,casein has high nutritional value.It mainly consists of four types:αs1-casein,αs2-casein,β-casein and κ-casein,and the characteristics of the four conformations are different.Casein can produce more active peptides after enzymatic digestion or digestion,which have good effects on regulating oxidative stress,lowering blood pressure,promoting mineral absorption,lowering blood sugar and blood lipid,improving antibacterial property and regulating nervous system.The current preparation methods of active peptides are mainly divided into chemical degradation,enzymatic digestion,microbial fermentation,genetic engineering and chemical synthesis,and each method has corresponding advantages and disadvantages.In order to promote the development of bioactive peptides from casein in milk,this paper provided a comprehensive overview of the composition characteristics of casein,types of active peptides and preparation methods,with the aim of promoting the industrialization of active peptides from casein and enhancing the added value of milk.

Key words: casein, antioxidant, hypotension, enzymatic digestion

[1] 刘兵.不同品种牛乳营养成分含量及脂肪酸含量和组成测定比较研究[J].畜牧与饲料科学,2022,43(5):93-97.
[2] 宫霞,郭本恒,李云飞,等.乳酪蛋白活性肽的开发趋势及应用前景[J].乳业科学与技术,2005(1):1-4,11-18.
[3] 刘永峰,张薇,刘婷婷,等.乳蛋白中乳清蛋白与酪蛋白组成、特性及应用的研究进展[J].食品工业科技,2020,41 (23):354-358.
[4] 于洋,祁艳霞,靳艳.乳源生物活性肽研究进展[J].食品与发酵工业,2017,43(9):259-266.
[5] 张源淑,邹思湘,陈伟华.酪蛋白酶解产物阿片样活性的细胞生物学鉴定[J].中国乳品工业,2000(4):36-38.
[6] Liu C,Liu LX,Yang J,et al.Exploration and analysis of the composition and mechanism of efficacy of camel milk[J].Food Bioscience,2023,53:102564.
[7] 刘晶晶,张薇,吴锦涛,等.酪蛋白结构特性及应用研究进展[J].食品研究与开发,2023,44(1):197-201.
[8] 瓦文强,秦娟娟,杨敏,等.酪蛋白胶束乳液凝胶性质及其在大黄素负载中的应用[J].食品与发酵工业,2023,49 (1):132-139.
[9] Horne D S.Casein micelles as hard spheres:Limitations of the model in acidified gel formation[J].Colloids and Surfaces A:Physicochemical and Engineering Aspects,2003,213 (2):255-263.
[10] Hann M,Böhm W,Drichel S,et al.Acid-induced gelation of enzymatically and nonenzymatically cross-linked caseins-Texture properties,and microstructural insights[J].Journal of Agricultural and Food Chemistry,2020,68(47):13970-13981.
[11] 肖仕杰,王巧华,樊懿楷,等.傅里叶变换红外光谱的牛乳中αs1-酪蛋白和κ-酪蛋白含量的快速检测[J].光谱学与光谱分析,2021,41(12):3688-3694.
[12] Mcmahon D J,Oommen B S.Supramolecular structure of the casein micelle[J].Journal of Dairy Science,2008,91(5): 1709-1721.
[13] Duerasch A,Herrmann P,Hogh K,et al.Study on β-casein depleted casein micelles:Micellar stability,enzymatic cross-linking,and suitability as nanocarriers[J].Journal of Agricultural and Food Chemistry,2020,68(47):13940-13949.
[14] 刘玲.VPP/IPP源酪蛋白水解物及类似物改善高脂饮食诱导肥胖小鼠胰岛素抵抗和炎症作用研究[D]. 杭州:浙江大学, 2021.
[15] 亓艺洁.蛋白酶辅助乳酸菌发酵水解酪蛋白制备抗氧化肽的研究[D].哈尔滨:东北农业大学,2020.
[16] Shu G W,Mei S,Chen L,et al.Optimization of the antioxiant peptides production from goat milk casein hydrolyzed by alcalase and papain using respons surface methodology[J].Food Technology,2019,43(1):24-39.
[17] 秦娟娟,何超群,刘金龙,等.酪蛋白的胰蛋白酶水解物分离及其抗氧化性研究[J].中国酿造,2019,38(11):102-106.
[18] 汤海霞,王爽爽,郝果,等.一种新型绵羊乳酪蛋白ACE抑制肽结构鉴定及分子结合机制分析[J].食品工业科技, 2022,43(1):110-118.
[19] Tu M L,Wang C,Chen C,et al.Identification of a novel ACE-inhibitory peptide from casein and evaluation of the inhibitory mechanisms[J].Food Chemistry,2018,256:98-104.
[20] Adams C,Sawh F,Green-Johnson J M,et al.Characterization of casein-derived peptide bioactivity:Differential effects on angiotensin-converting enzyme inhibition,cytokine and nitric oxide production[J].Journal of Dairy Science,2020,103 (7):5805-5815.
[21] 薛海燕,薛丽欢,贺宝元,等.牛乳酪蛋白体外模拟消化液的ACE抑制活性及其肠道吸收[J].现代食品科技,2018,34 (6):9-17.
[22] Bougherra F,Dilmi-Bouras A,Balti R,et al.Antibacterial activity of new peptide from bovine casein hydrolyzed by a serine metalloprotease of Lactococcus lactis subsp lactis BR16[J].Journal of Functional Foods,2017,32:112-122.
[23] 赵琼,李素梅,陈昌航,等.贯筋藤蛋白酶水解槟榔江水牛酪蛋白制备抗菌肽[J].食品工业,2020,41(5):151-155.
[24] Esmaeilpour M,Ehsani M R,Aminlari M,et al.Antimicrobial activity of peptides derived from enzymatic hydrolysis of goat milk caseins[J].Comparative Clinical Pathology,2016,25 (3):599-605.
[25] Liao W,Liu S,Liu X,et al.The purification,identification and bioactivity study of a novel calcium-binding peptide from casein hydrolysate[J].Food & Function,2019,10(12): 7724-7732.
[26] 王倩倩,杜鹃,冯凤琴.不同水解度酪蛋白磷酸肽的理化性质和持钙能力比较[J].食品安全质量检测学报,2022,13 (5):1438-1443.
[27] 王艳青,王永胜,马立保.酪蛋白磷酸肽(CPP)对肉仔鸡矿物质代谢的影响[J].中国粮油学报,2008,23(4):179-184.
[28] Qian J,Zheng L,Su G,et al.Identification and screening of potential bioactive peptides with sleep-enhancing effects in bovine milk casein hydrolysate[J].Journal of Agricultural and Food Chemistry,2021,69(38):11246-11258.
[29] Ken K,Noriko S,Hirohiko N,et al.The effect of casein hydrolysate intake on cerebral neural regulation during cognitive tasks in the elderly[J].Experimental Gerontology, 2022,165:111853.
[30] 梁小慧,王孝治,赵佳园,等.酪蛋白源降胆固醇肽的酶解制备工艺优化[J].食品工业科技,2022,43(19):280-287.
[31] 王玲丽,刘同杰,张兰威,等.牦牛乳酪蛋白水解制备DPPIV抑制肽的蛋白酶发掘及其酶解工艺优化[J].食品与发酵工业,2021,47(15):137-141.
[32] 钱兢菁,郑淋,赵谋明.酪蛋白体外消化过程中DPP-IV抑制活性的变化规律及其机制分析[J].食品科学,2020,41 (15):186-193.
[33] Zhu H Y,Wu M,Yu F Q,et al.Chemical synthesis of thioether-bonded bicyclic peptides using tert-butylthio and Trt-protected cysteines[J].Tetrahedron Letters,2021,67: 152875.
[34] 李佳逸,阳辉蓉,陈炼红.牦牛乳酪蛋白酶解产物抗氧化活性研究[J].保鲜与加工,2022,22(2):76-82.
[35] 张爽,张兰威,韩雪.乳酸菌蛋白酶与发酵乳制品质量相关性研究进展[J].微生物学报,2015,55(12):1530-1536.
[36] Debra C,Harold S.Bioactive milk peptides:A prospectus[J]. Journal of Dairy Science,2000,83(6):1187-1195.
[37] Blanco-Canosa J B,Nardone B,Albericio F,et al.Chemical protein synthesis using a second-generation N-Acylurea Linker for the preparation of peptide-thioester precursors[J].Journal of the American Chemical Society, 2015,137(22):7197-7209.
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