基于气相色谱法的甜蜜素含量新旧国家标准测定方法对比研究

doi:10.12377/1671-4393.26.05.13

摘要/Abstract

摘要： [目的]通过市场调查,探究甜蜜素在饮料、风味发酵乳中的使用情况,从标准条文和结果测定两个维度,对气相色谱法测定甜蜜素含量的新旧国家标准方法进行比较分析,进而归纳总结试验注意事项,以期为检验人员提供参考。[方法]本研究于内蒙古自治区乌兰察布市集宁区开展市场调查,采用气相色谱法测定甜蜜素含量。为探究储存条件对甜蜜素稳定性的影响,试验对常温和冷藏两种储存条件下样品中甜蜜素含量的变化进行了测定和对比研究。[结果]约37%风味发酵乳中含有甜味剂;约47%饮料中含有甜味剂,其中甜蜜素的使用占比第三。新旧国家标准对其的检测结果差异不显著,最高检出值为0.509 g/kg,所测样品均合格。待测液在常温及冷藏条件下存储14 d后,其检测值变化较小,最大变异系数仅为2.13%,两种存储条件下无显著性差异（P>0.05）。[结论]新国家标准简化了操作步骤,缩短了检测时间,降低了对检验人员的要求,为测定类标准的更新提供了良好范例。待测液可稳定存储14 d,在常温和冷藏存储条件下无显著性差异,为甜蜜素含量的日常检测提供了参考。

关键词: 甜蜜素, 气相色谱法, 甜味剂, 标准

Abstract: [Objective] This study aimed to investigate the usage of sodium cyclamate in beverages and flavored fermented milk through market research. The study compared and analyzed the new and old national standards for the determination of sodium cyclamate content using gas chromatography,and summarized the test precautions to provide reference for inspectors. [Method] This study conducted a market survey in Jining District,Ulanqab City,Inner Mongolia Autonomous Region,using gas chromatography to determine the sodium cyclamate content.To explore the impact of storage conditions on the stability of sodium cyclamate,the changes in sodium cyclamate content in samples under normal temperature and refrigeration conditions were measured and compared. [Result] Market research has found that about 37% of flavored fermented milk contains sweeteners. Approximately 47% of beverages contained sweeteners,with sodium cyclamate usage accounting for the third. There was no significant difference in the detection results of the new and old national standards. The highest detected value was 0.509 g/kg,and all tested samples were qualified. After 14 days of storage at normal temperature and refrigeration conditions,the detection values of the tested liquid changed slightly,with the maximum coefficient of variation being only 2.13%,and there was no significant difference between the two storage conditions（P>0.05）. [Conclusion] The new national standard simplifies the operation steps,shortens the detection time,and reduces the requirements for inspectors,providing a good example for the update of similar standards.The tested liquid can be stored stably for 14 days,and there was no significant difference in storage conditions at normal temperature and refrigeration,providing a reference for the daily detection of sodium cyclamate content in beverages.

Key words: sodium cyclamate, gas chromatography, sweetener, standard

师新彩, 周芹, 李艺凡, 艾吉木. 基于气相色谱法的甜蜜素含量新旧国家标准测定方法对比研究[J]. 中国乳业, 2026, 0(5): 86-95.

SHI Xincai, ZHOU Qin, LI Yifan, Aijimu. Comparative Study on the Determination Methods of Sodium Cyclamate Content According to the Old and New National Standards Based on Gas Chromatography Technique[J]. China Dairy, 2026, 0(5): 86-95.

参考文献

[1] 吴鹏,张成云,朱旭丽,等.人工甜味剂对人体的影响及国内外标准现状探讨[J].食品科学,2024,45(15):383-392.
[2] 陈晓,王家赟,孙昊,等.常见非糖甜味剂对糖代谢影响的研究进展[J].中国食品添加剂,2024,35(10):220-226.
[3] Yang H,Hang L.Reaction of cyclamate with hypochlorous acid in vitro and formation of chlorcyclohexylamine and cyclohexylamine[J].Food Additives & Contaminants:Part A,2024,41(3):228-236.
[4] 毛伟峰,宋雁.食品中常见甜味剂使用方面存在的主要问题及危害[J].食品科学技术学报,2018,36(6):9-14.
[5] Chen Z,Chen G,Zhou K,et al.Toxicity of food sweetener-sodium cyclamate on osteoblasts cells[J].Biochemical and Biophysical Research Communications,2019,508(2):507-511.
[6] Hu Y,Xie M,Wu X.Interaction studies of sodium cyclamate with DNA revealed by spectroscopy methods[J].Spectrochimica Acta Part A:Molecular and Biomolecular Spectroscopy,2019,220,117085.
[7] Hasan H M,Alkass S Y,de Oliveira D S P.Impact of Long-term Cyclamate and Saccharin Consumption on Biochemical Parameters in Healthy Individuals and Type 2 Diabetes Mellitus Patients[J].Medicina,2023,59(4),698.
[8] Mbambo N P,Dlamini S N,Chukwuma C I,et al.Comparative effects of commonly used commercially available non-nutritive sweeteners on diabetes-related parameters in non-diabetic rats[J].Journal of Food Biochemistry,2020,44(11):e13453.
[9] Wei L,Ma D,Jiang G,et al.Effects of sodium cyclohexyl sulfamate on growth and development of drosophila melanogaster[J].Agricultural Biotechnology,2024,13(4):65-69.
[10] 马琳,祁琪,李雅轩,等.甜蜜素对果蝇繁殖生长及运动能力的影响[J].首都师范大学学报(自然科学版),2024,45(4):36-41.
[11] Pisera-Fuster A,Sofía Otero,Talevi A,et al.Anticonvulsant effect of sodium cyclamate and propylparaben on pentylenetetrazol-induced seizures in zebrafish[J].Synapse,2017,71(4):e21961.
[12] 彭子娟,薄梦,吴雪,等.食品甜味剂的检测及其标准物质研究现状[J].计量科学与技术,2023,67(9):40-48.
[13] 国家卫生和计划生育委员会.GB2760-2014 食品安全国家标准食品添加剂使用标准[S].
[14] 国家卫生健康委员会,国家市场监督管理总局.GB2760-2024.食品安全国家标准食品添加剂使用标准[S].
[15] 国家卫生健康委员会食品安全标准与监测评估司.关于金花茶培养物等11种“三新食品”的公告[EB/OL].https://www.nhc.gov.cn/sps/c100088/202412/ccde2b21bdb8482c9aa08ee54ac775eb.shtml,2024-12-5.
[16] 国家卫生健康委员会,国家市场监督管理总局.GB5009.97-2023.食品安全国家标准食品中环己基氨基磺酸盐的测定[S].
[17] 中华人民共和国国家卫生和计划生育委员会.GB5009.97-2016.食品安全国家标准食品中环己基氨基磺酸钠的测定[S].
[18] 赵云霞,刘冰,王怡,等.湖北省食品中甜蜜素和安赛蜜的风险评估[J].中国食品卫生杂志,2022,34(1):110-115.
[19] 陆婉瑶,赵芸,张思聪,等.食糖与代糖的博弈及发展趋势分析[J].甘蔗糖业,2021,50(3):80-93.
[20] Wang Y,Li C,Li D,et al.Estimated assessment of dietary exposure to artificial sweeteners from processed food in Nanjing,China[J].Food Additives & Contaminants:Part A,2021,38(7):1105-1117.
[21] Tan V W K,Wee M S M,Tomic O,et al.Temporal sweetness and side tastes profiles of 16 sweeteners using temporal check-all-that-apply(TCATA)[J].Food Research International,2019(121):39-47.
[22] 黄柯,郑积敏,杨春霞.无营养性甜味剂——替代糖[J].化学教育(中英文),2024,45(2):1-12.
[23] Behrens M,Blank K,Meyerhof W.Blends of non-caloric sweeteners saccharin and cyclamate show reduced off-taste due to TAS2R bitter receptor inhibition[J].Cell Chemical Biology,2017,24(10):1199-1204.e2.
[24] 徐鑫,牛宇敏,邵兵.北京市中小学生5 种食品添加剂的内暴露水平分析[J].中国食品卫生杂志,2024,36(5):550-556.
[25] 王艳莉,李成国,郭宝福,等.南京市居民膳食中甜蜜素暴露的风险评估[J].现代预防医学,2020,47(18):3322-3325,3357.
[26] 黄燕,徐文泱,虞岚,等.湖南省现制饮料中甜味剂膳食暴露及对青少年风险评估[J].食品与机械,2024,40(8):58-63.
[27] 蔡建华,方军,王李平,等.广东部分地区食品中环己基氨基磺酸钠的含量调查[J].食品安全质量检测学报,2021,12(4):1644-1649.
[28] 周荃. 甜蜜素诱导小鼠急性肝损伤模型的优化与评价[D].衡阳:南华大学,2020.
[29] 杨涛,殷超.气相色谱法测定含乳饮料中甜蜜素含量的不确定度评定[J].广州化工,2019,47(19):101-103.
[30] 景赞,刘超,刘晓碧.实验室检测苹果汁中甜蜜素的能力分析[J].中国果菜,2020,40(5):83-86.
[31] 刘志鹏,杨李胜,王小鹏,等.基于气相色谱法测定食品中甜蜜素的碱性水解法研究[J].食品研究与开发,2021,42(10):154-158.
[32] 董宪兵. 复配甜味剂在植物饮料中的应用研究[J].现代食品,2020(6):151-153.
[33] 夏熠珣,陈佳,王子元,等.甜味与其它基本味相互作用的研究进展[J].中国食品学报,2024,24(8):12-30.
[34] 常炯炯,雍凌,肖潇,等.我国食品甜味剂联合使用情况及累积风险评估[J].毒理学杂志,2021,35(3):184-192.

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