中国乳业 ›› 2025, Vol. 0 ›› Issue (9): 135-144.doi: 10.12377/1671-4393.25.09.20

• 乳品加工 • 上一篇    下一篇

牛奶中天然活性成分及其影响因素研究

孙华, 孙菁笛, 何金兴, 曲静然*   

  1. 齐鲁工业大学(山东省科学院)食品科学与工程学院,山东济南 250353
  • 出版日期:2025-09-25 发布日期:2025-10-09
  • 通讯作者: *曲静然(1970-),女,山东烟台人,硕士,副教授,研究方向为食品生物技术。
  • 作者简介:孙 华(1981-),女,山东肥城人,博士,实验师,研究方向为功能性食品的研究与开发;孙菁笛(1987-),女,山东威海人,硕士,讲师,研究方向为食品物性与组分;何金兴(1978-),男,山东临沂人,博士,教授,研究方向为食品加工过程质量控制。
  • 基金资助:
    横向课题[2024KY(JY)HT0414]; 齐鲁工业大学(山东省科学院)实验教学和创新实验室建设研究项目资助(2025syyb05)

Research on Natural Active Components in Milk and their Influencing Factors

SUN Hua, SUN Jingdi, HE Jinxing, QU Jingran*   

  1. School of Food Science and Engineering,Qilu University of Technology(Shandong Academy of Sciences),Jinan Shandong 250353
  • Online:2025-09-25 Published:2025-10-09

摘要: 牛奶是一种营养全面的天然食品,富含蛋白质、脂肪、碳水化合物、维生素和矿物质等多种基础营养成分,同时含有多种具有生理调节功能的天然活性成分。本文系统综述了牛奶中主要天然活性成分——共轭亚油酸(CLA)、α-亚麻酸(ALA)、二十碳五烯酸(EPA)、二十二碳六烯酸(DHA)、乳铁蛋白和免疫球蛋白的生物学功能及其健康效应,并重点分析了饲养方式、饲粮组成、季节变化、胎次及泌乳阶段等因素对其含量的影响,以期为科学饮奶与高附加值乳制品开发提供理论依据。

关键词: 牛奶, 共轭亚油酸(CLA), α-亚麻酸(ALA), 二十二碳六烯酸(DHA), 二十碳五烯酸(EPA), 乳铁蛋白, 免疫球蛋白

Abstract: Milk is a nutritionally complete natural food,rich in a variety of essential nutrients such as proteins,fats,carbohydrates,vitamins,and minerals,while also containing multiple natural active ingredients with physiological regulatory functions. This paper systematically reviewed the biological functions and health effects of key natural active components in milk—including conjugated linoleic acid (CLA),α-linolenic acid (ALA),eicosapentaenoic acid (EPA),docosahexaenoic acid (DHA),lactoferrin,and immunoglobulins—and emphasizes the impact of factors such as feeding methods,diet composition,seasonal variations,parity,and lactation stage on their content. The aim is to provide a theoretical basis for scientific milk consumption and the development of high-value-added dairy products.

Key words: milk, conjugated linoleic acid(CLA), α-linolenic acid(ALA), docosahexaenoic acid(DHA), eicosapentaenoic acid(EPA), lactoferrin, immunoglobulin

[1] Amalfitano N,Patel N,Haddi M L,et al.Detailed mineral profile of milk,whey,and cheese from cows,buffaloes,goats,ewes and dromedary camels,and efficiency of recovery of minerals in their cheese[J].Journal of Dairy Science,2024,107(11):8887-8907.
[2] Pereira P C.Milk nutritional composition and its role in human health[J].Nutrition,2014,30(6):619-627.
[3] Watson R R,Collier R J,Preedy V R.Nutrients in Dairy and their Implications on Health and Disease[M].,Amsterdam:Elsevier Inc,2017.
[4] 王加启,郑楠,李松励.中国奶产品质量安全研究报告(2017年度)[R].北京:中国农业科学技术出版社,2018.
[5] Achón M,Úbeda N,García-González Á,et al.Effects of milk and dairy product consumption on pregnancy and lactation outcomes:A systematic review[J].Advances in Nutrition,2019,10(suppl_2):S74-S87.
[6] Patil G B,Wani S P,Bafna P S,et al.Milk adulteration:From detection to health impact[J].Food and Humanity,2024,3:100339.
[7] 刘正清,王志坤,葛鑫,等.牛奶中酪蛋白的结构和作用研究进展[J].中国奶牛,2024(5):38-43.
[8] 王磊,成雪,毛学英.乳清蛋白及其活性多肽的生物学功能研究进展[J].中国农业科技导报,2010,12(5):30-35.
[9] Lopez C,Briard-Bion V,Ménard O,et al.Fat globules selected from whole milk according to their size:Different compositions and structure of the biomembrane,revealing sphingomyelin-rich domains[J].Food Chemistry,2011,125(2):355-368.
[10] Li T,Gao J,Du M,et al.Milk fat globule membrane attenuates high-fat diet-induced obesity by inhibiting adipogenesis and increasing uncoupling protein 1 expression in white adipose tissue of mice[J].Nutrients,2018,10(3):331.
[11] Yuan Q C,Zhan B Y,Du M,et al.Dietary milk fat globule membrane regulates JNK and PI3K/Akt pathway and ameliorates type 2 diabetes in mice induced by a high-fat diet and streptozotocin[J].Journal of Functional Foods,2019,60:103435.
[12] 姜佳齐,羊玉凤,李建锋,等.牛奶及乳脂摄入对脂质代谢影响的初步研究[J].基因组学与应用生物学,2024,43(11):1746-1760.
[13] Yan K,Ma X Y,Jiang M C,et al.Effects of bovine milk and buffalo milk on lipid metabolism in mice[J].J. Journal of animal physiology and animal nutrition,2023,107(2):428-434.
[14] Shingfield K J,Bonnet M,Scollan N D.Recent developments in altering the fatty acid composition of ruminant-derived foods[J].Animal,2013,7(s1):132-162.
[15] 倪炜,王梦琦,张少卿,等.荷斯坦牛与娟姗牛乳脂肪酸组成与测定日泌乳性能的差异分析[J].中国奶牛,2018(5):24-29.
[16] Khoshvaght A,Towhidi A,Zare-Shahneh A,et al.Dietary n-3 PUFAs improve fresh and postthaw semen quality in Holstein bulls via alteration of sperm fatty acid composition[J].Theriogenology,2016,85(5):807-812.
[17] Kabeya N,Fonseca M M,Ferrier D E K,et al.Genes for de novo biosynthesis of omega-3 polyunsaturated fatty acids are widespread in animals[J].Science Advances,2018, 4(5):eaar6849.
[18] Saini R K,Keum Y S.Omega-3 and omega-6 polyunsaturated fatty acids:Dietary sources,metabolism,and significance—A review[J].Life Sciences,2018,203:255-267.
[19] 刘玉鹏,夏佳,秦炯.Omega-3多不饱和脂肪酸对脑发育及脑功能的价值[J].中国实用儿科杂志,2023,38(10):741-745.
[20] Culussi G,Catena C,Novello M,et al.Impact of omega-3 polyunsaturated fatty acids on vascular function and blood pressure:Relevance for cardiovascular outcomes[J].Nutrition Metabolism And Cardiovascular Diseases,2017,27(3):191-200.
[21] Al-Ghannami S S,Al-Adawi S,Ghebremeskel K,et al.Randomised open-label trial of docosahexaenoic acid-enriched fish oil and fish meal on cognitive and behavioural functioning in Omani children[J].Nutrition,2019(57):167-172.
[22] 董人华,滕国新,王晓杰,等.牛乳低聚糖的结构和功能研究及其开发应用[J].中国乳品工业,2022,50(12):34-38.
[23] Badawy S,Liu Y,Guo M,et al Conjugated linoleic acid(CLA) as a functional food:Is it beneficial or not?[J].Food Research International,2023,172:113158.
[24] Viladomiu M,Hontecillas R,Bassaganya-Riera J.Modulation of inflammation and immunity by dietary conjugated linoleic acid[J].European Journal of Pharmacology,2016,785:87-95.
[25] Ali Y M,Kadir A A,Ahmad Z,et al.Free radical scavenging activity of conjugated linoleic acid as single or mixed isomers[J].Pharmaceutical Biology,2012,50(6):712-719.
[26] Chen Y,Yang B,Ross R P,et al.Orally administered CLA ameliorates DSS-induced colitis in mice via intestinal barrier improvement,oxidative stress reduction,and inflammatory cytokine and gut microbiota modulation[J].Journal of Agricultural and Food Chemistry,2019,67(48):13282-13298.
[27] Ren Q,Yang B,Zhang H,et al.C9,t11,c15-CLNA and t9,t11,c15-CLNA from Lactobacillus plantarum ZS2058 ameliorate dextran sodium sulfate-induced colitis in mice[J].Journal of Agricultural and Food Chemistry,2020,68(12):3758-3769.
[28] Jiang C Y,Zhang F,Zhang M,et al.Conjugated linoleic acid ameliorates necrotizing enterocolitis by suppressing inflammatory responses and maintaining intestinal barrier integrity via the PPARγ/NFκB signaling pathway[J].Journal of Functional Foods,2024,123:106581.
[29] Dilzer A,Park Y.Implication of conjugated linoleic acid(CLA) in human health[J].Critical Reviews in Food Science and Nutrition,2012,52(6):488-513.
[30] Hartigh L J.Conjugated linoleic acid effects on cancer,obesity,and atherosclerosis:A review of pre-clinical and human trials with current perspectives[J].Nutrients,2019,11(2):370.
[31] Derakhshande-Rishehri S M,Mansourian M,Kelishadi R,et al.Association of foods enriched in conjugated linoleic acid(CLA) and CLA supplements with lipid profile in human studies:A systematic review and meta-analysis[J].Public Health Nutrition,2014,18(11):2041-2054.
[32] 巩振虎,刘义章,周凯,等.α-亚麻酸的制备与分离技术研究进展[J].商丘师范学院学报,2021,37(12):24-27.
[33] Huang W,Hu M,Yuan Q,et al.The review of alpha-linolenic acid:Sources,metabolism,and pharmacology[J].Phytotherapy Research,2022,36:164-188.
[34] Leikin-Frenkel A,Schnaider B M,Cooper I.How alpha linolenic acid may sustain blood-brain barrier integrity and boost brain resilience against alzheimer’s disease[J].Nutrients,2022,14(23):5091.
[35] Leikin-Frenkel A,Liraz-Zaltsman S,Hollander K S,et al.Dietary alpha linolenic acid in pregnant mice and during weaning increases brain docosahexaenoic acid and improves recognition memory in the offspring[J].The Journal of Nutritional Biochemistry,2021,91:108597.
[36] De Lorgeril M,Renaud S,Salen P,et al.Mediterranean alpha-linolenic acid-rich diet in secondary prevention of coronary heart disease[J].Lancet,1994,343(8911):1454-1459.
[37] Li Y,Yu Z,Liu Y,et al.Dietar α-linolenic acid-rich flaxseed oil ameliorates high-fat diet-induced atherosclerosis via gut microbiotainflammation-artery Axis in ApoE-/-mice[J].Frontiers in Cardiovascular Medicine,2022,9:830781.
[38] Xi H,Zhou W,Sohaib M,et al.Flaxseed supplementation significantly reduces hemoglobin A1c in patients with type 2 diabetes mellitus:A systematic review and meta-analysis[J].Nutrition Research,2023,110:23-32.
[39] Liu Y Y,Guo M X,Li Y W,et al.α-Linolenic acid regulates macrophages via GPR120-NLRP3 inflammasome pathway to ameliorate diabetic rats[J]. Journal of Functional Foods,2022(99):105348.
[40] Gonzáalez-Fernández M J,Ortea I,Guil-Guerrero J L.α-Linolenic and γ-linolenic acids exercise differential antitumor effects on HT-29 human colorectal cancer cells[J].Toxicology Research,2020,9(4):474-483.
[41] Artym J,Zimecki M,Kruzel M L.Lactoferrin for prevention and treatment of anemia and inflammation in pregnant women:A comprehensive review[J].Biomedicines,2021,9(8):898.
[42] Liu L,Guo Q,Cui M.Impact of maternal nutrition during early pregnancy and diet during lactation on lactoferrin in mature breast milk[J].Nutrition(Burbank,Los Angeles County,Calif.),2022,93:111500.
[43] 许琳霜,屈晓清,曾小凤,等.天然和热变性乳铁蛋白与α-乳白蛋白聚集体的超分子结构表征[J].食品工业科技,2020,41(15):15-20,28.
[44] Dierick M,Vanrompay D,Devriendt B,et al.Minireview:Lactoferrin,a versatile natural antimicrobial glycoprotein which modulates host innate immunity[J].Biochemistry and Cell Biology,2020,99(2):61-65.
[45] Hu C,Shen W,Xia Y,et al.Lactoferrin:Current situation and future prospects[J].Food Bioscience,2024,62:105183.
[46] Sawale M,Singh A,Gutierrez V,et al.Antimicrobial effect of lactoferrin and glycerol monolaurate on selected bacteria associated with newborn infectionss[J].Food Control,2025(168):110882.
[47] Sherman M P,Sherman J,Arcinue R,et al.Randomized control trial of human recombinant lactoferrin:A substudy reveals effects on the fecal microbiome of very low birth weight infants[J].Journal of Pediatrics,2016,173:37-42.
[48] Alkhulaifi M M,Alosaimi M M,Khan M S,et al.Assessment of broadspectrum antimicrobial,antibiofilm,and anticancer potential of lactoferrin extracted from camel milk[J].Applied Biochemistry and Biotechnology,2024,196(3):1464-1480.
[49] 中国医疗保健国际交流促进会营养与代谢管理分会.乳铁蛋白临床应用中国专家共识[J].中华预防医学杂志,2022,56(12):1694-1701.
[50] 吴洪亚,高亚男,王加启,等.乳铁蛋白对肠道屏障保护作用的研究进展[J].现代食品科技,2023,39(11):371-380.
[51] Manzoni P,Meyer M,Stolfi I,et al.Bovine lactoferrin supplementation for prevention of necrotizing enterocolitis in very-low-birth-weight neonates:A randomized clinical trial[J].Early Human Development,2014,90(Suppl 1):S60-S65.
[52] Ochoa T J,Chea-Woo E,Baiocchi N,et al.Randomized double-blind controlled trial of bovine lactoferrin for prevention of diarrhea in children[J].Journal Of Pediatrics,2013,162(2):349-356.
[53] 邱金玲,成一新,刘小凡.乳铁蛋白对Dex模型小鼠肠道菌群调控研究[J].中国食物与营养,2023,29(7):52-56.
[54] Lee O Y A,Wong A N N,Ho C Y,et al.Potentials of natural antioxidants in reducing inflammation and oxidative stress in chronic kidney disease[J].Antioxidants,2024,13(6):751.
[55] Wright S W,Lovelace-Macon L,Ducken D,et al.Lactoferrin is a dynamic protein in human melioidosis and is a TLR4-dependent driver of TNF-α release in Burkholderia thailandensis infection in vitro[J].PLoS Neglected Tropical Diseases,2020,14(8):e0008495.
[56] Liu C,Peng Q,Wei L,et al.Deficiency of lactoferrin aggravates lipopolysaccharide-induced acute inflammation via recruitment macrophage in mice[J].Biometals,2023,36(3):549-562.
[57] Ran L,Shi J,Lin Y,et al.Evaluation of the protective bioactivity and molecular mechanism verification of lactoferrin in an Alzheimer’s mouse model with ulcerative enteritis[J].Journal of Dairy Science,2024,107(11):8796-8810.
[58] Li W,Liu B,Lin Y,et al.The application of lactoferrin in infant formula:The past,present and future[J].Critical Reviews in Food Science and Nutrition, 2024,64(17):5748-5767.
[59] Ramírez‐Sánchez D A,Canizalez‐Román A,León‐Sicairos N,et al.The anticancer activity of bovine lactoferrin is reduced by deglycosylation and it follows a different pathway in cervix and colon cancer cells[J].Food Science & Nutrition,2024,12(5):3516-3528.
[60] 肖红艳,兰欣怡,张佩华.乳铁蛋白生物学功能研究进展[J].中国奶牛,2021(4):46-50.
[61] 皮迪,王小凤,刘志娟.婴幼儿配方奶粉中主要蛋白质及其主要功能的研究概况[J].湘南学院学报(医学版),2022,24(4):75-78.
[62] Alothman M,Hogan S A,Hennessy D,et al.“Grass-Fed” milk story:Understanding the impact of pasture feeding on the composition and quality of bovine milk[J].Foods,2019,8(8):350.
[63] 贺越,赵圣国,郑楠,等.α-亚麻酸的生理功能及其在牛奶中的营养调控研究进展[J].中国乳品工业,2020,48(6):31-35.
[64] 张克春,徐国忠,沈向真.放牧奶牛的牛奶脂肪酸营养价值分析[J].乳业科学与技术,2010,33(1):37-38.
[65] 孙小琴. 放牧奶牛乳脂肪酸组成及瘤胃脂肪酸代谢规律的研究[D].咸阳:西北农林科技大学,2012.
[66] 梁俊芳. 牛乳中乳铁蛋白随着季节变化规律的研究[J].农产品加工·学刊,2009(2):75-76.
[67] 杨晋辉,张军民,卜登攀,等.不同牛场春季和夏季牛奶中IgA、IgM和乳铁蛋白的调查[J].华中农业大学学报,2013,32(3):94-98.
[68] 张静静,李春芳,樊懿楷,等.牛奶中天然活性物质和过敏物质及其影响因素[J]. 中国奶牛,2021(6):41-46.
[69] Cheng J B,Wang J Q,Bu D P,et al.Factors affecting the lactoferrin concentration in bovine milk[J].Journal of Dairy Science,2008,91(3):970-976.
[70] Tortadès M,Garcia-Fruitós E,Arís A,et al.Short communication:The biological value of transition milk:analyses of immunoglobulin G,IGF-I and lactoferrin in primiparous and multiparous dairy cows[J].Animal,2023,17(7):100861.
[71] Acosta-Balcazar I C,Quiroz-Valiente J,Granados-Zurita L,et al.Effect of genotype,lactation and climatic factors on fatty acid profile of bovine milk[J].Czech Journal of Animal Science,2022,67(5):167-175.
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