中国乳业 ›› 2025, Vol. 0 ›› Issue (9): 135-144.doi: 10.12377/1671-4393.25.09.20
孙华, 孙菁笛, 何金兴, 曲静然*
SUN Hua, SUN Jingdi, HE Jinxing, QU Jingran*
摘要: 牛奶是一种营养全面的天然食品,富含蛋白质、脂肪、碳水化合物、维生素和矿物质等多种基础营养成分,同时含有多种具有生理调节功能的天然活性成分。本文系统综述了牛奶中主要天然活性成分——共轭亚油酸(CLA)、α-亚麻酸(ALA)、二十碳五烯酸(EPA)、二十二碳六烯酸(DHA)、乳铁蛋白和免疫球蛋白的生物学功能及其健康效应,并重点分析了饲养方式、饲粮组成、季节变化、胎次及泌乳阶段等因素对其含量的影响,以期为科学饮奶与高附加值乳制品开发提供理论依据。
| [1] Amalfitano N,Patel N,Haddi M L,et al.Detailed mineral profile of milk,whey,and cheese from cows,buffaloes,goats,ewes and dromedary camels,and efficiency of recovery of minerals in their cheese[J].Journal of Dairy Science,2024,107(11):8887-8907. [2] Pereira P C.Milk nutritional composition and its role in human health[J].Nutrition,2014,30(6):619-627. [3] Watson R R,Collier R J,Preedy V R.Nutrients in Dairy and their Implications on Health and Disease[M].,Amsterdam:Elsevier Inc,2017. [4] 王加启,郑楠,李松励.中国奶产品质量安全研究报告(2017年度)[R].北京:中国农业科学技术出版社,2018. [5] Achón M,Úbeda N,García-González Á,et al.Effects of milk and dairy product consumption on pregnancy and lactation outcomes:A systematic review[J].Advances in Nutrition,2019,10(suppl_2):S74-S87. [6] Patil G B,Wani S P,Bafna P S,et al.Milk adulteration:From detection to health impact[J].Food and Humanity,2024,3:100339. [7] 刘正清,王志坤,葛鑫,等.牛奶中酪蛋白的结构和作用研究进展[J].中国奶牛,2024(5):38-43. [8] 王磊,成雪,毛学英.乳清蛋白及其活性多肽的生物学功能研究进展[J].中国农业科技导报,2010,12(5):30-35. [9] Lopez C,Briard-Bion V,Ménard O,et al.Fat globules selected from whole milk according to their size:Different compositions and structure of the biomembrane,revealing sphingomyelin-rich domains[J].Food Chemistry,2011,125(2):355-368. [10] Li T,Gao J,Du M,et al.Milk fat globule membrane attenuates high-fat diet-induced obesity by inhibiting adipogenesis and increasing uncoupling protein 1 expression in white adipose tissue of mice[J].Nutrients,2018,10(3):331. [11] Yuan Q C,Zhan B Y,Du M,et al.Dietary milk fat globule membrane regulates JNK and PI3K/Akt pathway and ameliorates type 2 diabetes in mice induced by a high-fat diet and streptozotocin[J].Journal of Functional Foods,2019,60:103435. [12] 姜佳齐,羊玉凤,李建锋,等.牛奶及乳脂摄入对脂质代谢影响的初步研究[J].基因组学与应用生物学,2024,43(11):1746-1760. [13] Yan K,Ma X Y,Jiang M C,et al.Effects of bovine milk and buffalo milk on lipid metabolism in mice[J].J. Journal of animal physiology and animal nutrition,2023,107(2):428-434. [14] Shingfield K J,Bonnet M,Scollan N D.Recent developments in altering the fatty acid composition of ruminant-derived foods[J].Animal,2013,7(s1):132-162. [15] 倪炜,王梦琦,张少卿,等.荷斯坦牛与娟姗牛乳脂肪酸组成与测定日泌乳性能的差异分析[J].中国奶牛,2018(5):24-29. [16] Khoshvaght A,Towhidi A,Zare-Shahneh A,et al.Dietary n-3 PUFAs improve fresh and postthaw semen quality in Holstein bulls via alteration of sperm fatty acid composition[J].Theriogenology,2016,85(5):807-812. [17] Kabeya N,Fonseca M M,Ferrier D E K,et al.Genes for de novo biosynthesis of omega-3 polyunsaturated fatty acids are widespread in animals[J].Science Advances,2018, 4(5):eaar6849. [18] Saini R K,Keum Y S.Omega-3 and omega-6 polyunsaturated fatty acids:Dietary sources,metabolism,and significance—A review[J].Life Sciences,2018,203:255-267. [19] 刘玉鹏,夏佳,秦炯.Omega-3多不饱和脂肪酸对脑发育及脑功能的价值[J].中国实用儿科杂志,2023,38(10):741-745. [20] Culussi G,Catena C,Novello M,et al.Impact of omega-3 polyunsaturated fatty acids on vascular function and blood pressure:Relevance for cardiovascular outcomes[J].Nutrition Metabolism And Cardiovascular Diseases,2017,27(3):191-200. [21] Al-Ghannami S S,Al-Adawi S,Ghebremeskel K,et al.Randomised open-label trial of docosahexaenoic acid-enriched fish oil and fish meal on cognitive and behavioural functioning in Omani children[J].Nutrition,2019(57):167-172. [22] 董人华,滕国新,王晓杰,等.牛乳低聚糖的结构和功能研究及其开发应用[J].中国乳品工业,2022,50(12):34-38. [23] Badawy S,Liu Y,Guo M,et al Conjugated linoleic acid(CLA) as a functional food:Is it beneficial or not?[J].Food Research International,2023,172:113158. [24] Viladomiu M,Hontecillas R,Bassaganya-Riera J.Modulation of inflammation and immunity by dietary conjugated linoleic acid[J].European Journal of Pharmacology,2016,785:87-95. [25] Ali Y M,Kadir A A,Ahmad Z,et al.Free radical scavenging activity of conjugated linoleic acid as single or mixed isomers[J].Pharmaceutical Biology,2012,50(6):712-719. [26] Chen Y,Yang B,Ross R P,et al.Orally administered CLA ameliorates DSS-induced colitis in mice via intestinal barrier improvement,oxidative stress reduction,and inflammatory cytokine and gut microbiota modulation[J].Journal of Agricultural and Food Chemistry,2019,67(48):13282-13298. [27] Ren Q,Yang B,Zhang H,et al.C9,t11,c15-CLNA and t9,t11,c15-CLNA from Lactobacillus plantarum ZS2058 ameliorate dextran sodium sulfate-induced colitis in mice[J].Journal of Agricultural and Food Chemistry,2020,68(12):3758-3769. [28] Jiang C Y,Zhang F,Zhang M,et al.Conjugated linoleic acid ameliorates necrotizing enterocolitis by suppressing inflammatory responses and maintaining intestinal barrier integrity via the PPARγ/NFκB signaling pathway[J].Journal of Functional Foods,2024,123:106581. [29] Dilzer A,Park Y.Implication of conjugated linoleic acid(CLA) in human health[J].Critical Reviews in Food Science and Nutrition,2012,52(6):488-513. [30] Hartigh L J.Conjugated linoleic acid effects on cancer,obesity,and atherosclerosis:A review of pre-clinical and human trials with current perspectives[J].Nutrients,2019,11(2):370. [31] Derakhshande-Rishehri S M,Mansourian M,Kelishadi R,et al.Association of foods enriched in conjugated linoleic acid(CLA) and CLA supplements with lipid profile in human studies:A systematic review and meta-analysis[J].Public Health Nutrition,2014,18(11):2041-2054. [32] 巩振虎,刘义章,周凯,等.α-亚麻酸的制备与分离技术研究进展[J].商丘师范学院学报,2021,37(12):24-27. [33] Huang W,Hu M,Yuan Q,et al.The review of alpha-linolenic acid:Sources,metabolism,and pharmacology[J].Phytotherapy Research,2022,36:164-188. [34] Leikin-Frenkel A,Schnaider B M,Cooper I.How alpha linolenic acid may sustain blood-brain barrier integrity and boost brain resilience against alzheimer’s disease[J].Nutrients,2022,14(23):5091. [35] Leikin-Frenkel A,Liraz-Zaltsman S,Hollander K S,et al.Dietary alpha linolenic acid in pregnant mice and during weaning increases brain docosahexaenoic acid and improves recognition memory in the offspring[J].The Journal of Nutritional Biochemistry,2021,91:108597. [36] De Lorgeril M,Renaud S,Salen P,et al.Mediterranean alpha-linolenic acid-rich diet in secondary prevention of coronary heart disease[J].Lancet,1994,343(8911):1454-1459. [37] Li Y,Yu Z,Liu Y,et al.Dietar α-linolenic acid-rich flaxseed oil ameliorates high-fat diet-induced atherosclerosis via gut microbiotainflammation-artery Axis in ApoE-/-mice[J].Frontiers in Cardiovascular Medicine,2022,9:830781. [38] Xi H,Zhou W,Sohaib M,et al.Flaxseed supplementation significantly reduces hemoglobin A1c in patients with type 2 diabetes mellitus:A systematic review and meta-analysis[J].Nutrition Research,2023,110:23-32. [39] Liu Y Y,Guo M X,Li Y W,et al.α-Linolenic acid regulates macrophages via GPR120-NLRP3 inflammasome pathway to ameliorate diabetic rats[J]. Journal of Functional Foods,2022(99):105348. [40] Gonzáalez-Fernández M J,Ortea I,Guil-Guerrero J L.α-Linolenic and γ-linolenic acids exercise differential antitumor effects on HT-29 human colorectal cancer cells[J].Toxicology Research,2020,9(4):474-483. [41] Artym J,Zimecki M,Kruzel M L.Lactoferrin for prevention and treatment of anemia and inflammation in pregnant women:A comprehensive review[J].Biomedicines,2021,9(8):898. [42] Liu L,Guo Q,Cui M.Impact of maternal nutrition during early pregnancy and diet during lactation on lactoferrin in mature breast milk[J].Nutrition(Burbank,Los Angeles County,Calif.),2022,93:111500. [43] 许琳霜,屈晓清,曾小凤,等.天然和热变性乳铁蛋白与α-乳白蛋白聚集体的超分子结构表征[J].食品工业科技,2020,41(15):15-20,28. [44] Dierick M,Vanrompay D,Devriendt B,et al.Minireview:Lactoferrin,a versatile natural antimicrobial glycoprotein which modulates host innate immunity[J].Biochemistry and Cell Biology,2020,99(2):61-65. [45] Hu C,Shen W,Xia Y,et al.Lactoferrin:Current situation and future prospects[J].Food Bioscience,2024,62:105183. [46] Sawale M,Singh A,Gutierrez V,et al.Antimicrobial effect of lactoferrin and glycerol monolaurate on selected bacteria associated with newborn infectionss[J].Food Control,2025(168):110882. [47] Sherman M P,Sherman J,Arcinue R,et al.Randomized control trial of human recombinant lactoferrin:A substudy reveals effects on the fecal microbiome of very low birth weight infants[J].Journal of Pediatrics,2016,173:37-42. [48] Alkhulaifi M M,Alosaimi M M,Khan M S,et al.Assessment of broadspectrum antimicrobial,antibiofilm,and anticancer potential of lactoferrin extracted from camel milk[J].Applied Biochemistry and Biotechnology,2024,196(3):1464-1480. [49] 中国医疗保健国际交流促进会营养与代谢管理分会.乳铁蛋白临床应用中国专家共识[J].中华预防医学杂志,2022,56(12):1694-1701. [50] 吴洪亚,高亚男,王加启,等.乳铁蛋白对肠道屏障保护作用的研究进展[J].现代食品科技,2023,39(11):371-380. [51] Manzoni P,Meyer M,Stolfi I,et al.Bovine lactoferrin supplementation for prevention of necrotizing enterocolitis in very-low-birth-weight neonates:A randomized clinical trial[J].Early Human Development,2014,90(Suppl 1):S60-S65. [52] Ochoa T J,Chea-Woo E,Baiocchi N,et al.Randomized double-blind controlled trial of bovine lactoferrin for prevention of diarrhea in children[J].Journal Of Pediatrics,2013,162(2):349-356. [53] 邱金玲,成一新,刘小凡.乳铁蛋白对Dex模型小鼠肠道菌群调控研究[J].中国食物与营养,2023,29(7):52-56. [54] Lee O Y A,Wong A N N,Ho C Y,et al.Potentials of natural antioxidants in reducing inflammation and oxidative stress in chronic kidney disease[J].Antioxidants,2024,13(6):751. [55] Wright S W,Lovelace-Macon L,Ducken D,et al.Lactoferrin is a dynamic protein in human melioidosis and is a TLR4-dependent driver of TNF-α release in Burkholderia thailandensis infection in vitro[J].PLoS Neglected Tropical Diseases,2020,14(8):e0008495. [56] Liu C,Peng Q,Wei L,et al.Deficiency of lactoferrin aggravates lipopolysaccharide-induced acute inflammation via recruitment macrophage in mice[J].Biometals,2023,36(3):549-562. [57] Ran L,Shi J,Lin Y,et al.Evaluation of the protective bioactivity and molecular mechanism verification of lactoferrin in an Alzheimer’s mouse model with ulcerative enteritis[J].Journal of Dairy Science,2024,107(11):8796-8810. [58] Li W,Liu B,Lin Y,et al.The application of lactoferrin in infant formula:The past,present and future[J].Critical Reviews in Food Science and Nutrition, 2024,64(17):5748-5767. [59] Ramírez‐Sánchez D A,Canizalez‐Román A,León‐Sicairos N,et al.The anticancer activity of bovine lactoferrin is reduced by deglycosylation and it follows a different pathway in cervix and colon cancer cells[J].Food Science & Nutrition,2024,12(5):3516-3528. [60] 肖红艳,兰欣怡,张佩华.乳铁蛋白生物学功能研究进展[J].中国奶牛,2021(4):46-50. [61] 皮迪,王小凤,刘志娟.婴幼儿配方奶粉中主要蛋白质及其主要功能的研究概况[J].湘南学院学报(医学版),2022,24(4):75-78. [62] Alothman M,Hogan S A,Hennessy D,et al.“Grass-Fed” milk story:Understanding the impact of pasture feeding on the composition and quality of bovine milk[J].Foods,2019,8(8):350. [63] 贺越,赵圣国,郑楠,等.α-亚麻酸的生理功能及其在牛奶中的营养调控研究进展[J].中国乳品工业,2020,48(6):31-35. [64] 张克春,徐国忠,沈向真.放牧奶牛的牛奶脂肪酸营养价值分析[J].乳业科学与技术,2010,33(1):37-38. [65] 孙小琴. 放牧奶牛乳脂肪酸组成及瘤胃脂肪酸代谢规律的研究[D].咸阳:西北农林科技大学,2012. [66] 梁俊芳. 牛乳中乳铁蛋白随着季节变化规律的研究[J].农产品加工·学刊,2009(2):75-76. [67] 杨晋辉,张军民,卜登攀,等.不同牛场春季和夏季牛奶中IgA、IgM和乳铁蛋白的调查[J].华中农业大学学报,2013,32(3):94-98. [68] 张静静,李春芳,樊懿楷,等.牛奶中天然活性物质和过敏物质及其影响因素[J]. 中国奶牛,2021(6):41-46. [69] Cheng J B,Wang J Q,Bu D P,et al.Factors affecting the lactoferrin concentration in bovine milk[J].Journal of Dairy Science,2008,91(3):970-976. [70] Tortadès M,Garcia-Fruitós E,Arís A,et al.Short communication:The biological value of transition milk:analyses of immunoglobulin G,IGF-I and lactoferrin in primiparous and multiparous dairy cows[J].Animal,2023,17(7):100861. [71] Acosta-Balcazar I C,Quiroz-Valiente J,Granados-Zurita L,et al.Effect of genotype,lactation and climatic factors on fatty acid profile of bovine milk[J].Czech Journal of Animal Science,2022,67(5):167-175. |
| [1] | 徐大江, 马占峰, 孙艳, 赵丽娟, 高一娜, 杨青青. 基于中红外光谱对牛乳中乳铁蛋白含量的快速检测[J]. 中国乳业, 2025, 0(7): 115-120. |
| [2] | 刘来可, 刘桂芹. 绵羊乳腺免疫防御机制研究进展[J]. 中国乳业, 2025, 0(6): 71-75. |
| [3] | 刘瑛, 王湘竹, 马鹏举, 张轶轩, 赵亮军, 秦虹. 婴儿配方奶粉中DHA和ARA的科学设计范围研究[J]. 中国乳业, 2025, 0(5): 122-127. |
| [4] | 赵凤茹, 谢凯丽, 水明, 丛慧敏, 薛文强, 李琪琪, 代晶晶, 龙洋, 王婼依. 测定牛奶和奶粉中甲基毒死蜱残留量的气相色谱串联质谱法研究[J]. 中国乳业, 2025, 0(4): 97-103. |
| [5] | 张加稳, 张联琴, 刘羽晏, 左诚, 王蕊, 刁玉华. 不同杀菌温度对巴氏杀菌乳品质的影响[J]. 中国乳业, 2025, 0(3): 71-76. |
| [6] | 张婧, 朱孔迪, 刘传铭, 刘长振, 王鹏杰, 黄家强. 一种快速精准检测各种乳品中牛乳铁蛋白含量的广适性ELISA方法[J]. 中国乳业, 2025, 0(3): 91-99. |
| [7] | 葛强. 超声波监控装置及自动化控制系统的研发[J]. 中国乳业, 2024, 0(9): 90-96. |
| [8] | 钱科婷. 公共事件中行政执法的优化路径研究——以奶业事件为例[J]. 中国乳业, 2024, 0(9): 105-110. |
| [9] | 白学兵. 热应激对奶牛泌乳性能影响的研究[J]. 中国乳业, 2024, 0(8): 98-102. |
| [10] | 周欢, 宋艳梅, 范光彩, 夏忠悦, 马静, 骆敏, 谭莲英, 钱成林. 福林酚法高通量测定牛奶中蛋白酶活力的优化[J]. 中国乳业, 2024, 0(7): 92-97. |
| [11] | 曾云凤, 周振新, 刘昨, 陈芳, 李文静, 陈宝宇, 李伟胤, 卜泽明. 盖勃法测定牛奶中脂肪含量的优化[J]. 中国乳业, 2024, 0(7): 98-101. |
| [12] | 文冬雪, 陈小成, 何明锦, 蓝嘉辉, 韦锦超, 农达, 孔志伟. 荷斯坦牛奶和水牛奶不同配比对奶酪品质的影响[J]. 中国乳业, 2024, 0(6): 115-119. |
| [13] | 王英南, 卢智华, 李艳红, 伊德润, 毕成名, 何青春, 李慧, 王斌, 白晓玲. 火焰原子吸收光谱法检测牛奶中钙方法的研究[J]. 中国乳业, 2024, 0(4): 61-66. |
| [14] | 弓耀忠, 孙丽萍, 曾品国, 郭忠强, 尚强胜, 王媛慧, 杜利君, 巩丽青. 灭菌乳酸度指标扩大必要性的研究[J]. 中国乳业, 2024, 0(4): 72-76. |
| [15] | 窦佩佩, 张慧娟, 卢涵, 袁庆彬, 王世杰, 罗永康. A2β-酪蛋白牛奶和普通牛奶的体外消化特性和抗氧化能力评价[J]. 中国乳业, 2024, 0(4): 85-90. |
|
||